Cell viability was determined by using Cell Counting Kit-8 (CCK-8, FDbio Science, Hangzhou, China) according to the manufacturer’ instructions on a plate reader at 450 nm.
Cell counting kit 8 (cck8)
Manufactured by Fdbio
Sourced in China
The Cell Counting Kit-8 is a colorimetric assay for the determination of cell viability and cell proliferation. It uses a water-soluble tetrazolium salt to measure the number of viable cells in a sample.
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3 protocols using cell counting kit 8 (cck8)
1
Cell Viability Measurement Using CCK-8
2
Cell Viability Assessment via CCK-8
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Cell Counting Kit-8 (CCK-8) assay (Fdbio Science, China) was used to measure cell viability. After transfection, LN229 cells were seeded into a 96-well cell plate at a density of 2 × 103 cells/well; the absorbance values were detected 0–4 days after transfection, and 10 μl of CCK-8 solution was added daily, followed by incubation for 2 h. Then, a microplate reader (Thermo) was used to measure the absorbance at 450 nm.
3
Cell Viability Assay using CCK-8
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Cell viability was measured by using Cell Counting Kit-8 (CCK-8, FDbio Science, Hangzhou, China) according to the manufacturer' instructions. Briefly, eEPCs were seeded in 96-well plates at 1 × 104 cells/well in the presence or absence of r-TNFα (PeproTech Inc., Rocky Hill, NJ, USA). At the indicated time points, 10 μl of CCK8 solution was added into each well, followed by incubation of the plates at 39 °C in 5% CO2 for 2 h. The optical density (OD) was measured at 450 nm.
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