Immunofluorescence staining of HBEC was performed as previously described20 ,25 (link). ALI cultures were fixed with a solution of 50%/50% methanol/acetone for 2 min at – 20 °C followed by three washes with PBS. Fixed cultures were incubated with anti-MUC5AC antibody (MA1-38223; Thermo Fisher Scientific) at 0.4 µg/mL and anti-MUC5B antibody (#37-7400; Thermo Fisher Scientific) at 2 µg/mL overnight at 4 °C. Cultures were then incubated with Alexa Fluor 555 donkey anti-mouse IgG (#A31570; Thermo Fisher Scientific) at 1 µg/mL and Alexa Fluor 488 donkey anti-rabbit IgG (#A21206; Thermo Fisher Scientific) at 1 µg/mL for 1 h at room temperature. Anti-acteylated α-tubulin antibody (#T6793; MilliporeSigma) was used at 1:1000 dilution overnight at 4 °C. Hoechst 33258 (#H3569; Thermo Fisher Scientific) was used at 2 µg/mL for 10 min at room temperature.
Anti muc5ac antibody
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Anti-MUC5AC antibody is a laboratory research tool used to detect the presence and expression levels of the MUC5AC protein in biological samples. MUC5AC is a secreted mucin glycoprotein involved in the formation of mucus. This antibody can be used in various immunoassay techniques to identify and quantify MUC5AC in cell lines, tissues, or other sample types.
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Lab products found in correlation
Alexa fluor 555 donkey anti mouse igg, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 488 donkey anti rabbit igg, by Thermo Fisher Scientific (1 mentions)
Hoechst 33258, by Thermo Fisher Scientific (1 mentions)
Hoechst, by Thermo Fisher Scientific (1 mentions)
C2 confocal microscope, by Nikon (1 mentions)
2 protocols using anti muc5ac antibody
1
Immunofluorescence Staining of HBEC
2
Immunofluorescence Staining of Airway Epithelium
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Immunofluorescence staining of tissue sections was performed as described [21 (link)]. Tissue sections were incubated with anti-MUC5AC antibody (no. MA1-38223, Thermo Fisher Scientific, Waltham, MA, USA) at 0.4 μg·mL−1 overnight at 4°C and with Hoechst (no. H3569, Thermo Fisher Scientific) at 2 μg·mL−1 for 10 min. For staining of P1 HBECs, ALI cultures on Transwell inserts were fixed with a solution of 50%/50% methanol/acetone for 2 min at −20°C followed by three washes with PBS. A 3% BSA solution was used to block for 1 h before incubation with primary antibodies. P1 HBECs exposed to CS or room air were washed and fixed 48 h after exposure following the same immunostaining protocol. All slides were imaged with a Nikon C2+ confocal microscope (Nikon Instruments, Tokyo, Japan).
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