Bacterial DNA was isolated from fresh (24 h) tryptic soy broth (BioRad, Hercules, CA, USA) cultures prepared from single colonies growing on tryptic soy agar (BioRad, Hercules, CA, USA). Isolation of DNA was performed by spin column method according to the manufacturer’s instruction (Genomic Mini, A&A Biotechnology, Gdynia, Poland). Bacterial DNA was stored at 4 °C for further investigation.
Tryptic soy agar
Manufactured by Bio-Rad
Sourced in United States, France
Tryptic soy agar is a general-purpose microbiological growth medium used for the cultivation of a wide range of bacteria. It provides the essential nutrients and growth factors required for the proliferation of various bacterial species.
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Lab products found in correlation
2 protocols using tryptic soy agar
1
Bacterial DNA Isolation from Tryptic Soy Broth
2
Isolation and Identification of Imipenem-Ceftazidime Resistant Pseudomonas aeruginosa
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A total of 80 imipenem–ceftazidime-resistant P. aeruginosa (ICRPA) isolates were retrospectively selected from the frozen stocks kept in brain heart infusion with 20% glycerol at −80 °C at the microbial laboratory of Charles Nicolle hospital of Tunis during a period of four years (2014 to 2017).
From frozen stock, each isolate was subcultured twice on tryptic soy agar (Biorad, Marnesta la Coquette, France), incubating each subculture in ambient air at 35 °C ± 2 °C for 18 to 24 h to ensure purity and viability. Thus, only 35 non-duplicated clinical ICRPA isolates were successfully subcultured and included in this study.
Bacterial identification was performed using the API 20NE system (bioMérieux, Marcy l’Etoile, France).
From frozen stock, each isolate was subcultured twice on tryptic soy agar (Biorad, Marnesta la Coquette, France), incubating each subculture in ambient air at 35 °C ± 2 °C for 18 to 24 h to ensure purity and viability. Thus, only 35 non-duplicated clinical ICRPA isolates were successfully subcultured and included in this study.
Bacterial identification was performed using the API 20NE system (bioMérieux, Marcy l’Etoile, France).
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