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7 protocols using sodium glycocholate

1

Synthesis and Characterization of Metal Oxide Nanoparticles

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Cobalt nitrate (CoNO3.6H2O), aluminum nitrate (AlNO3.9H2O), Zinc nitrate (ZnNO3.6H2O) bi-distilled water, urea, citric acid, trisodium salt, butyl alcohol, sodium hydroxide (NaOH), sodium nitrate (NaNO3), tween 20, sodium glycocholate, and oleic acid were purchased from Alfa Aesar, Germany. Essential oils of a commercially available jasmine oil blend consisting of Jasmine oil and Azores jasmine (J. sambac and J. azoricum, respectively) (Oleaceae) and peppermint (M. arvensis) (Lamiaceae) were bought from “Cap Pharm” EL CAPTAIN Company for extracting natural oils, plants, and cosmetics (El Obor, Cairo, Egypt); we would refer to the used oils as AJ and PP, respectively. All chemicals and essential oils were used without further purification or distillation.
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2

Characterization of Sandalwood Oil Phenolics

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Tween 20, sodium glycocholate and sodium cholate hydrate were obtained from Alfa Aesar, Germany. Sandalwood oil purchased from the local market in jazan. Gallic acid, 3.4-dihydroxybenzoic acid, chlorogenic acid, catechin, caffeic acid, methyl gallate, syringic acid, coumaric acid, vanillin, rutin, ellagic acid, ferulic acid, myricetin, daidzein, luteolin, quercetin, naringenin, apegenin, kaempferol and hesperetin as phenolic compounds standards were purchased from Sigma Aldrich, USA. All reagents were HPLC grade.
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3

Formulation and Characterization of Lipid Nanocarriers

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Oleic acid 90%, polysorbate 20 (Tween 20), Sodium Glycocholate 97.5%, Sodium Cholate 99%, and Distilled water (de-ionized), all chemicals were purchased from Alfa Aesar, Karlsruhe, Germany, and used with no further purification. Chemicals used for biochemical analysis were the Bovine albumin standard, purchased from Stanbio Laboratory (Texas, USA); Commasie brilliant blue G-250, purchased from Sigma (Sigma Chemical Co.); P- nitroanisole (purity 97%), acquired from Ubichem Ltd. (Ham pshire); and nicotinamide ademine dinucleotide phosphate (reduced form, NADPH), got from BDH chemicals Ltd. (Poole, England). The rest of the chemicals were of high quality purchased from commercial local companies and used without further purification.
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4

Methylene Blue Nanoparticle Synthesis

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Methylene blue (MB) dye 89%, Stearic acid 97%, oleic acid 90%, De-ionized water, polysorbate 20 (tween20), sodium glycocholate 97.5% and sodium tourocholate, 96% were purchased from Alfa Aesar (Thermo Fisher Scientific, Germany), while citronellal 93% and carvacrol 95% monoterpens were obtained from Acros Organics (Thermo Fisher Scientific, Germany). All the chemical reagents were used without any purification.
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5

Curcumin-Based Nanoformulation Development

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Curcumin (Cur) 97%, oleic acid 90%, stearic acid 97%, polysorbate 20 (tween 20), sodium taurocholate 96%, sodium glycocholate 97.5%, hydrogen peroxide 29%, and deionized water were procured from Alfa Aesar (Thermo Fisher Scientific, Dreieich, Germany), whereas linalool and geraniol were procured from Acros Organics (Thermo Fisher Scientific, Dreieich, Germany). Skweez (contains Imidacloprid 34%) insecticide was purchased locally from Semadak (Damanhur, El Beheira governorate Egypt). All chemical reagents were utilized without being purified, with the exception of Skweez insecticide, which was lyophilized to produce a powder.
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6

Danazol-Sodium Glycocholate Encapsulation

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Danazol was purchased from Spectrum Chemicals. Sodium glycocholate was obtained from ACROS Organics. Type 2 glass beads used for the technique were purchased from Glenmills Inc. Capsules (size 2) were obtained from Capsugel, USA. All materials, reagents, and solvents were purchased from commercial sources and used without further purification.
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7

Diclofenac Sodium Nanomedicine Development

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Diclofenac sodium (DNa) was kindly provided by Misr Pharmaceutical Co. (Cairo, Egypt). Span 40 (sorbitan nonopalmitate) and Span 60 (sorbitan monostearate) were kindly supplied from CID Co. (Cairo, Egypt). Sodium cholate (SC), sodium deoxycholate (SDC), and sodium glycocholate (SGC) were purchased from Acros Organics (Cairo, Egypt). Cholesterol, lecithin and carrageenan (catalog number: C1013) were procured from Sigma-Aldrich (St. Louis, MO, USA). Methanol (HPLC grade) and methylene chloride (HPLC grade) were purchased El-NASR Pharmaceutical Chemicals Co. (Cairo, Egypt). Dialysis bags (Mol. Wt. cut off = 12,000 Da) were acquired from SERVA Electrophoresis GmbH (Heidelberg, Germany). Hemooxygenase 1 (HO-1) (catalog number: MBS8800478), cyclooxygenase 2 (COX-2) (catalog number: MBS7611878), IL-6 (catalog number: MBS370473), and nuclear factor-erythroid factor2-related factor 2 (Nrf-2) (catalog number: MBS012148) reagent kits were obtained as the rat ELISA kit from My Biosource, Inc. (San Diego, Southern California, CA, USA). All reagents were of analytical grade and commercially available.
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