Dlin mc3 dma
DLin-MC3-DMA is a lipid formulation component used in the development of lipid nanoparticle (LNP) drug delivery systems. It is a cationic lipid that can be used to facilitate the encapsulation and delivery of nucleic acid-based therapeutics, such as mRNA and siRNA.
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9 protocols using dlin mc3 dma
Lipid Nanoparticle Synthesis and Characterization
Lipid-nanoparticle mRNA Encapsulation Protocol
Formulation and Characterization of Lipid Nanoparticles for mRNA Delivery
Chikungunya Virus mRNA-LNP Production
For LNP production, D-Lin-MC3-DMA (MedChemExpress), DSPC (Avanti Polar Lipids), cholesterol (Sigma), and PEG-lipid (Avanti Polar Lipids) were solubilized in ethanol respectively and mixed with a molar ratio of 50:10:38.5:1.5. The lipid mixture was then mixed with an aqueous buffer (50 mmol/L citrate buffer [pH 4.0]), reaching the final lipid concentration of 7.37 mg/mL. Then the lipid mixture and mRNA were added into the NanoAssemblr™ with the volume ratio of 1:3 to obtain mRNA-LNP. Then the diluted LNP-encapsulated mRNA samples were concentrated to appropriate volume and passed through 0.22 μm filters. The produced mRNA-LNP was stored at 4 °C until use. The encapsulation efficiency was measured with the Quant-iT RiboGreen RNA Assay Kit (Life Technologies) using a microplate reader.
Preparation of mRNA-Loaded Lipid Nanoparticles
Lipid Nanoparticle Formulation Protocol
Polyamine-Based Lipid Nanoparticle Synthesis
Formulation of Lipid Nanoparticles for Delivery
Lipid-based Nanoparticle Synthesis
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