Human PCa (LNCaP, VCaP, C4‐2B, 22Rv1, DU145 and PC‐3) and human embryonic kidney 293T cell lines were obtained from the American Type Culture Collection. The cells were cultured under standard conditions as previously described.
21 (link) Cells were grown in phenol red‐free Roswell Park Memorial Institute (RPMI)‐1640 medium (Corning) supplemented with 10% charcoal‐stripped serum (Hyclone), mimicking an androgen‐deprived environment for experimental purposes. Cells were treated with Thiamet G (MedChemExpress) at a final concentration of 25 µM, OSMI‐1 (MedChemExpress) at a final concentration of 20 µM, MDV3100 (MedChemExpress) at a final concentration of 10 µM, dihydrotestosterone (DHT, Sigma–Aldrich) at a final concentration of 10 nM, actinomycin D (MedChemExpress) at a final concentration of 5 µg/mL, cycloheximide (CHX) at a final concentration of 10 µg/mL, BAY1251152 (Selleckchem) at a final concentration of 10 µM and uridine diphospho (UDP)‐GlcNAc (Sigma) at a final concentration of 60 mM. All cell lines were authenticated with short tandem repeat profiling and accepted regular tests for mycoplasma contamination.
21 (link) Cells were grown in phenol red‐free Roswell Park Memorial Institute (RPMI)‐1640 medium (Corning) supplemented with 10% charcoal‐stripped serum (Hyclone), mimicking an androgen‐deprived environment for experimental purposes. Cells were treated with Thiamet G (MedChemExpress) at a final concentration of 25 µM, OSMI‐1 (MedChemExpress) at a final concentration of 20 µM, MDV3100 (MedChemExpress) at a final concentration of 10 µM, dihydrotestosterone (DHT, Sigma–Aldrich) at a final concentration of 10 nM, actinomycin D (MedChemExpress) at a final concentration of 5 µg/mL, cycloheximide (CHX) at a final concentration of 10 µg/mL, BAY1251152 (Selleckchem) at a final concentration of 10 µM and uridine diphospho (UDP)‐GlcNAc (Sigma) at a final concentration of 60 mM. All cell lines were authenticated with short tandem repeat profiling and accepted regular tests for mycoplasma contamination.