Goat anti rrm2

All chemicals and reagents were purchased from Sigma-Aldrich (St. Louis, MO). pLKO.1-shRNA plasmids were obtained from Open Biosystems (Waltham, MA). The mature sense sequences are: shRRM2: 5′-CGGAGGAGAGAGTAAGAGAAA-3′; shATM #1: 5′-CGTGTCTTAATGAGACTACAA-3′; shATM #2: 5′-TGATGGTCTTAAGGAACATCT-3′; shp53: 5′-GAGGGATGTTTGGGAGATGTA-3′; and shc-MYC: 5′-CCTGAGACAGATCAGCAACAA-3. The following antibodies were obtained from the indicated suppliers: mouse anti-phospho-ATM (Rockland, Gilbertsville, PA), goat anti-ATM (Bethyl, Montgomery, TX), goat anti-RRM2 (Santa Cruz Biotechnology), mouse anti-Cyclin A (Novocastra), mouse anti-γH2AX (Millipore, Billerica, MA), rabbit anti-53BP1 (Bethyl), mouse anti-BrdU FITC (BD Biosciences, San Jose, CA), mouse anti-p53 (Calbiochem, Billerica, MA), rabbit anti-G6PD (Sigma-Aldrich), rabbit anti-p21 (Abcam, Cambridge, MA), rabbit anti-c-MYC (Cell Signaling, Danvers, MA), and mouse anti-β-actin (Sigma-Aldrich).

The vemurafenib analog PLX4720 was provided by Plexxikon (Berkeley, CA). pLKO.1-shRRM2 plasmids were obtained from Open Biosystems (Waltham, MA). The following antibodies were obtained from the indicated suppliers: goat anti-RRM2 (Santa Cruz Biotechnology, Santa Cruz, CA), mouse anti-cyclin A (Novocastra Laboratories, Buffalo Grove, IL), mouse anti-γH2AX (Millipore), rabbit anti-cleaved lamin A (Cell Signaling, Danvers, MA), rabbit anti-c-Myc (Cell Signaling), rabbit anti-PARP p85 (Promega Corporation, Fitchburg, WI), rabbit anti-phospho-ERK1/2 (Cell Signaling), mouse anti-GAPDH (Millipore), rabbit anti-pS10H3 (Millipore), and mouse anti-BrdU FITC (BD Biosciences).