Image studio digits v 5

The quantitative data of protein expression were obtained using the band scanner Image Studio Digits v. 5.2 (LI-COR Biosciences). While the quantitative data of mRNA expression were calculated using ImageJ 1.52v (NIH, Bethesda, MD, USA). The measurements were assessed in triplicate, and data were presented as mean standard deviation. After calculation of the normality of the data using a stem and leaf plot, one-way ANOVA followed with Tukey’s post hoc test was performed to assess the statistical significance of more than two groups. The differences were considered statistically significant when the P-value was <0.05. The statistical assessment for mRNA and protein expression was performed using IBM SPSS statistic 26 (IBM Corp., NY, USA).

Quantitative data was obtained using the band scanner Image Studio Digits v. 5.2 (LI-COR Biosciences). Data are presented as mean ± standard deviation. After calculation of the normality of the data using a stem and leaf plot, one-way ANOVA followed with Tukey's post hoc test was performed to assess the statistical significance of more than two groups. The measurements were assessed in triplicate. SPSS version 26 (IBM Corp.) was used for all statistical analyses. P<0.05 was considered to indicate a statistically significant difference.