Anti phospho sting ser366

Manufactured by Cell Signaling Technology

Sourced in China

The Anti-Phospho-STING (Ser366) antibody is a laboratory reagent used for the detection and analysis of phosphorylated STING (Stimulator of Interferon Genes) at serine 366. This antibody can be used in various experimental techniques, such as Western blotting, to study the phosphorylation status of STING, a key signaling protein involved in the activation of the innate immune response.

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Lab products found in correlation

Anti irf3, by Cell Signaling Technology (2 mentions) Anti sting, by Proteintech (2 mentions) Anti sting, by Cell Signaling Technology (2 mentions) Anti phospho nf kb p65 ser536, by Cell Signaling Technology (1 mentions) Anti cd63, by ABclonal (1 mentions) Anti alix 12422 1 ap, by Proteintech (1 mentions) Anti cd81 66866 1 ig, by Proteintech (1 mentions) Anti phospho sting ser365, by Cell Signaling Technology (1 mentions) Anti nf kb p65, by ABclonal (1 mentions) Anti phospho irf3 ser396, by Cell Signaling Technology (1 mentions) Anti β actin, by Cell Signaling Technology (1 mentions) Anti gapdh, by Beyotime (1 mentions) Anti calnexin, by Abcam (1 mentions) Protease inhibitor cocktail, by Keygen Biotech (1 mentions) Pierce bca protein assay kit, by Keygen Biotech (1 mentions) Phosphatase inhibitor cocktail, by Keygen Biotech (1 mentions) Ripa buffer, by Keygen Biotech (1 mentions) Anti cgas, by Cell Signaling Technology (1 mentions) Ifn λ1, by BioLegend (1 mentions) Anti sars cov 2 nucleocapsid, by GeneTex (1 mentions)

3 protocols using anti phospho sting ser366

Proteomic Analysis of Colon Tissue Fractions

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Colon tissues, cells, and EVs fractions were homogenized using the RIPA buffer (Jiangsu KeyGEN BioTECH, Nanjing, China), phosphatase inhibitor cocktail, and protease inhibitor cocktail (Jiangsu KeyGEN BioTECH, Nanjing, China). Pierce BCA Protein Assay Kit (Jiangsu KeyGEN BioTECH, Nanjing, China) was used to determine the protein concentration. Anti-CD63 (A5271, ABclonal), anti-Alix (12422-1-AP, Proteintech), anti-CD81 (66866-1-Ig, Proteintech), anti-Calnexin (ab22595, Abcam), anti-STING (19851-1-AP, Proteintech), anti-Phospho-STING (Ser366) (85735, Cell Signaling Technology), anti-Phospho-STING (Ser365) (72971, Cell Signaling Technology), anti-IRF3 (4302, Cell Signaling Technology), anti-Phospho-IRF3 (Ser396) (29047, Cell Signaling Technology), anti-NF-kB p65 (A19653, ABclonal), anti-Phospho-NF-kB p65 (Ser536) (3303, Cell Signaling Technology), anti-Beta-Actin (4970, Cell Signaling Technology), anti-GAPDH (AF1186, Beyotime Biotechnology) and secondary antibodies (KGP1201, Jiangsu KeyGEN BioTECH, Nanjing, China) were used for western blot.

Zhao F., Zheng T., Gong W., Wu J., Xie H., Li W., Zhang R., Liu P., Liu J., Wu X., Zhao Y, & Ren J. (2021). Extracellular vesicles package dsDNA to aggravate Crohn’s disease by activating the STING pathway. Cell Death & Disease, 12(9), 815.

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SARS-CoV-2 Signaling Pathway Analysis

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IFNβ and IFNλ1 were purchased from Biolegend. PAMPs were purchased from InvivoGen. diABZI, ruxolitinib and H-151 were purchased from Selleck Chemicals. The following antibodies were used for Western blotting or immunofluorescence: anti-RIG-I (Cell Signaling Technology, 3743S), anti-MDA5 (Cell Signaling Technology, 5321S), anti-MAVS (Cell Signaling Technology, 3993S), anti-cGAS (Cell Signaling Technology, 83623S), anti-STING (Cell Signaling Technology, 13647S), anti-STING (Proteintech, 19851-1-AP), anti-Phospho-STING (Ser366) (Cell Signaling Technology, 50907S), anti-TBK1 (Cell Signaling Technology, 3504S), anti-Phospho-TBK1 (Ser172) (Cell Signaling Technology, 5483S), anti-IRF3 (Cell Signaling Technology, 11904S), anti-Phospho-IRF3 (Ser396) (Cell Signaling Technology, 4947S), anti-NF-κB p65 (Cell Signaling Technology, 8242S), anti-Phospho-NF-κB p65 (Ser536) (Cell Signaling Technology, 3033S), anti-TRIM22 (Invitrogen, PA5-51964), anti-SARS-CoV-2 nucleocapsid (GeneTex, GTX135357), and anti-tubulin (Sigma, T6199). Anti-SARS-CoV-2 Spike antibody (CR3022) was from Absolute Antibody. HRP-conjugated secondary antibodies (anti-mouse or anti-rabbit) were purchased from Amersham. Alexa Fluor-conjugated secondary antibodies were purchased from Invitrogen.

Otto H., Hanson P.I, & Jahn R. (1997). Assembly and disassembly of a ternary complex of synaptobrevin, syntaxin, and SNAP-25 in the membrane of synaptic vesicles. Proceedings of the National Academy of Sciences of the United States of America, 94(12), 6197-6201.

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Western Blot Protein Detection

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Following protein transfer, nitrocellulose membranes were probed with protein-specific primary antibodies diluted in PBS containing 5% Milk or 5% BSA as follows: anti-actin Cat #A5441 from Sigma Aldrich; anti-tubulin Cat #5286 from Santa Cruz; anti-Flag M2 Cat #SLBF6631V from Sigma Aldrich; anti-Phospho-STING (Ser 366 ) Cat #19781 from Cell signaling; anti-STING Cat #13647 from Cell signaling; anti-Phospho-TBK1 (Ser 172 ) Cat #5483 from Cell signaling; anti-TBK1/IKKε Cat #IMG270A from Imgenex. Membranes were washed using dPBS containing 0.5% Tween (PBS-T) before incubation with horseradish peroxidase (HRP)-conjugated secondary antibodies (Seracare or Jackson Immunoresearch Laboratories). After final wa shes, enhanced chemiluminescence using the Western Lightning Chemiluminescence Reagent Plus (Perkin-Elmer Life Sciences) was performed to identify immunoreactive bands detected using a LAS4000mini CCD camera apparatus (GE healthcare).

Cuervo N.Z., Fortin A., Caron E., Chartier S., & Grandvaux N. (2020). Pinpointing of cysteine oxidation sites in vivo by high-resolution proteomics reveals mechanism of redox-dependent inhibition of STING.

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