Dulbecco’s Modified Eagle Medium Nutrient Mixture F-12 (DMEM/F-12) and antibiotic–antimycotic (penicillin G and streptomycin) solution were purchased from Gibco, Invitrogen, USA. Fetal bovine serum (FBS) was procured from Himedia, India. Hoechst 33342, propidium iodide (PI), Ribonuclease A (RNase A), 2,7-dichlorodihydrofluorescein diacetate (DCFH-DA), acridine orange (AO), ethidium bromide (EtBr) and rhodamine 123 (Rh 123) were purchased from Himedia, India. Annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) apoptosis detection kit was supplied by BioVision, USA. The antibodies used in western blotting against β-actin, p53, Bax, Bcl-2, cleaved caspase-3, AKT, p-AKT473, mTOR, p-mTOR2448 were purchased from Cell Signaling Technology (Danvers, MA). Other chemicals were obtained from Sigma-Aldrich, USA and were of analytical grade.
Rhodamine 123 rh 123
Manufactured by HiMedia
Sourced in India
Rhodamine 123 (Rh 123) is a fluorescent dye commonly used in various laboratory applications. It is a cationic dye that exhibits green fluorescence when excited by light. Rh 123 is often used as a stain for mitochondria in live cells, as it accumulates in the mitochondria due to the negative membrane potential.
Automatically generated - may contain errors
Lab products found in correlation
Hoechst 33342, by HiMedia (3 mentions)
Ethidium bromide etbr, by HiMedia (3 mentions)
Fetal bovine serum (fbs), by HiMedia (2 mentions)
Bcl2 associated x (bax), by Cell Signaling Technology (2 mentions)
Pakt473, by Cell Signaling Technology (2 mentions)
Propidium iodide ribonuclease a rnase a 2 7 dichlorodihydrofluorescein diacetate dcfh da acridine orange, by HiMedia (2 mentions)
P mtor2448, by Cell Signaling Technology (2 mentions)
Cleaved caspase 3, by Cell Signaling Technology (2 mentions)
Streptomycin, by Thermo Fisher Scientific (2 mentions)
Penicillin g, by Thermo Fisher Scientific (2 mentions)
Bcl 2, by Cell Signaling Technology (2 mentions)
β actin, by Cell Signaling Technology (2 mentions)
Dulbecco s modified eagle medium nutrient mixture f 12 dmem f 12, by Thermo Fisher Scientific (1 mentions)
Annexin 5 fluorescein isothiocyanate fitc propidium iodide apoptosis detection kit, by Abcam (1 mentions)
Low melting agarose, by Merck Group (1 mentions)
Dmem medium, by Merck Group (1 mentions)
Caspase 9, by Merck Group (1 mentions)
Caspase 3, by Merck Group (1 mentions)
Penicillin streptomycin, by Merck Group (1 mentions)
Phosphate buffered saline (pbs), by Merck Group (1 mentions)
4 protocols using rhodamine 123 rh 123
1
DMEM/F-12 Media Cytotoxicity Assay
2
Apoptosis Induction and Molecular Mechanism
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Acridine orange (AO), Hoechst stain, Ethidium Bromide and Rhodamine-123 (Rh-123) were purchased from Himedia, India. Cell culture chemicals such as heat inactivated fetal bovine serum (FBS), DMEM medium, glutamine, penicillin-streptomycin, EDTA, trypsin, phosphate buffered saline (PBS), low melting agarose, normal melting agarose; p53, Bcl-2, Bax, caspase-9, caspase-3 and Cytochrome c monoclonal antibodies were purchased from Sigma chemical Co., St. Louis, USA. All other chemicals and solvents were obtained from Fisher Inorg., Aromatic Limited Chennai and from S.D Fine Chemical Mumbai.
3
Cytotoxicity and Oxidative Stress Assays
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Dulbecco’s Modified Eagle Medium Nutrient Mixture F-12 (DMEM/F-12), fetal bovine serum (FBS), antibiotic penicillin and streptomycin solution and β-D-glucan powder were purchased from Sigma-Aldrich, USA. Hoechst 33342, propidium iodide (PI), Ribonuclease A (RNase A), 2,7-dichlorodihydrofluorescein diacetate (DCFH-DA), acridine orange (AO), ethidium bromide (EtBr) and rhodamine 123 (Rh 123) were purchased from Himedia, India. All other chemicals and reagents used were of analytical grade.
4
Apoptosis Induction Assay in Cells
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Dulbecco's Modi ed Eagle Medium Nutrient Mixture F-12 (DMEM/F-12) and antibiotic-antimycotic (penicillin G and streptomycin) solution were purchased from Gibco, Invitrogen, USA. Fetal bovine serum (FBS) was procured from Himedia, India. Hoechst 33342, propidium iodide (PI), Ribonuclease A (RNase A), 2,7-dichlorodihydro uorescein diacetate (DCFH-DA), acridine orange (AO), ethidium bromide (EtBr) and rhodamine 123 (Rh 123) were purchased from Himedia, India. Annexin V-uorescein isothiocyanate (FITC)/propidium iodide (PI) apoptosis detection kit was supplied by BioVision, USA. The antibodies used in western blotting against β-actin, p53, Bax, Bcl-2, cleaved caspase-3, Akt, p-Akt 473 , mTOR, p-mTOR 2448 were purchased from Cell Signaling Technology (Danvers, MA). Other chemicals were obtained from Sigma-Aldrich, USA and were of analytical grade.
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