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Fitc anti mouse cd25

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FITC anti-mouse CD25 is a fluorescently labeled antibody that binds to the mouse CD25 protein, also known as the IL-2 receptor alpha chain. This product can be used for the identification and analysis of CD25-expressing cells in mouse samples.

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Lab products found in correlation

Foxp3 buffer set, by BD (1 mentions) Pe anti mouse cd4, by BioLegend (1 mentions) Apc anti mouse cd4, by BioLegend (1 mentions) Percp anti mouse cd19, by BioLegend (1 mentions) Calibur, by BD (1 mentions) Collagenase, by Merck Group (1 mentions) Apc anti mouse cd3e, by BioLegend (1 mentions) Fitc anti mouse cd45, by BioLegend (1 mentions) Apc anti mouse cd8a, by Cytek Biosciences (1 mentions) Apc anti mouse ly 6g ly 6c, by BioLegend (1 mentions) Trustain fcx, by BioLegend (1 mentions) Pe anti mouse f4 80, by BioLegend (1 mentions) Sh800s cell sorter, by Sony (1 mentions)

Spelling variants of this product

CD25-FITC (33 citations) Anti-mouse CD25 (9 citations) Anti-CD25-FITC (8 citations) FITC-labeled anti-mouse CD25 (clone PC61) rat IgG1λ (2 citations)

3 protocols using fitc anti mouse cd25

1

Quantitative Analysis of Regulatory T Cells

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For evaluating the frequency of Treg cells, splenocytes were first surface stained with PE anti-mouse CD4 (Biolegend) and FITC anti-mouse CD25 (Biolegend). After permeabilization with Foxp3 buffer set (BD Biosciences), cells were incubated with PerCP/Cy5.5 anti-mouse Foxp3 for intracellular staining. Finally, Data were acquired and analyzed with FACSCalibur flow and flowJo software (Version 10).
Ahmadvand Koohsari S., Absalan A, & Azadi D. (2021). Human umbilical cord mesenchymal stem cell-derived extracellular vesicles attenuate experimental autoimmune encephalomyelitis via regulating pro and anti-inflammatory cytokines. Scientific Reports, 11, 11658.
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2

Single-cell Spleen Immune Profiling

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To acquire single-cell suspension, the mouse spleen was minced through a 40µm nylon cell strainer, and we used the Tris-NH4Cl lysis buffer (0.017 M Tris-HCl, 0.144 M NH4Cl) to deplete the red blood cells. Then the spleen cells were harvested and divided into two tubes, and 100μL blood was dripped into a tube for testing. One tube of the sample was incubated with the antibody FITC anti-mouse CD3, APC anti-mouse CD4, PerCP anti-mouse CD8, PE anti-mouse CD28, and the other tube of cells were incubated with APC anti-mouse CD4, FITC anti-mouse CD25, and PerCP anti-mouse CD19 (BioLegend) at room temperature for 30 min in the dark before being washed and resuspended in 0.5 mL of PBS and quantified by flow cytometry (BD Calibur™, USA).
Zeng J., Zhang X., Wang J., Cheng X., Zhang Y, & Zhou W. (2020). Comparison of Donepezil, Memantine, Melatonin, and Liuwei Dihuang Decoction on Behavioral and Immune Endocrine Responses of Aged Senescence-Accelerated Mouse Resistant 1 Mice. Frontiers in Pharmacology, 11, 350.
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3

Murine Submandibular Lymph Node Isolation

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Submandibular lymph nodes were extracted from BALB/c mice after euthanasia. Lymph node tissues were treated with 2 mg/mL collagenase (Sigma C0130) for 1 hr at 37°C and triturated with a pipet tip. Cell suspensions were filtered through a 70 um mesh, resuspended in FACS buffer (PBS, 5% FBS, 0.05% sodium azide), and counted by hemocytometer. 106 cells from each sample were aliquoted into V-bottom 96-well plates for subsequent staining. FC-receptors were blocked using TruStain fcX (101319, Biolegend, San Diego, CA), according to the manufacturer’s protocol. The following conjugated primary antibodies from Biolegend were used for cell surface staining: APC anti-mouse CD3e (100311), FITC anti-mouse CD25 (101907), FITC anti-mouse CD45 (103107), APC anti-mouse Ly-6G/Ly-6C (108411), PE anti-mouse F4/80 (123109). The following conjugated primary antibodies from Tonbo Biosciences (San Diego, CA) were used for cell surface staining: PE anti-mouse CD4 (50-0042), APC anti-mouse CD8a (20-1886). Cells were immunolabeled, washed, and analyzed with a SH800S Cell Sorter (Sony Biotechnology Inc., San Jose, CA).
Agelidis A.M., Hadigal S.R., Jaishankar D, & Shukla D. (2017). Viral Activation of Heparanase Drives Pathogenesis of Herpes Simplex Virus-1. Cell reports, 20(2), 439-450.
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