Cells were seeded onto glass coverslips and incubated with TI (each 10 ng/mL) in the absence or presence of BHSST (500 μg/mL) for 30 min. The cells were fixed in 4% paraformaldehyde and 100% acetone, blocked in 0.5% bovine serum albumin, and incubated with anti-STAT1 antibody (Cell Signaling Tech.) for 1 h at room temperature. Then, FITC-conjugated anti-rabbit immunoglobulin G (IgG) antibody (Invitrogen, Carlsbad, CA, USA) was used as a secondary antibody. The immunostained cells were mounted with medium containing DAPI and visualized by use of an Olympus FLUOVIEW FV10i confocal microscope (Tokyo, Japan).
Fitc conjugated anti rabbit immunoglobulin g igg antibody
Manufactured by Thermo Fisher Scientific
Sourced in United States
The FITC-conjugated anti-rabbit immunoglobulin G (IgG) antibody is a fluorescently labeled secondary antibody that binds to rabbit primary antibodies. The fluorescent label is fluorescein isothiocyanate (FITC), which emits green fluorescence when excited by the appropriate wavelength of light. This product is commonly used in immunoassays and immunofluorescence techniques to detect and visualize rabbit primary antibodies.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using fitc conjugated anti rabbit immunoglobulin g igg antibody
1
Quantifying STAT1 Translocation in Cells
2
Immunofluorescence Analysis of STAT1 in HaCaT Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
HaCaT cells were seeded onto glass coverslips and incubated with TI in the absence or presence of CSYJE (500 μg/mL) for 30 min. The cells were fixed in 4 % paraformaldehyde and 100 % acetone, blocked in 0.5 % bovine serum albumin, and incubated with anti-STAT1 antibody (Cell Signaling, Danvers, MA) for 1 h at room temperature. Then, fluorescein isothiocyanate (FITC)-conjugated anti-rabbit immunoglobulin G (IgG) antibody (Invitrogen, Carlsbad, CA) was used as a secondary antibody. The immunostained cells were mounted with medium containing 4′6-diamidino-2-phenylindole (DAPI) and visualized using an Olympus FLUOVIEW FV10i confocal microscope (Tokyo, Japan).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!