For immunoblot analyses, crude cell extracts were prepared and analyzed by SDS–PAGE as previously described (Hein et al., 1995 (link)). Proteins were transferred to a nitrocellulose membrane (Protran; Perkin Elmer) and probed with a mouse monoclonal anti-GFP (Roche), anti-hemagglutinin (anti-HA) (Roche), anti-yeast 3-phosphoglycerate kinase (anti-PGK; Invitrogen), anti-Ub (Santa Cruz; sc8017), or rabbit monoclonal anti-Pma1 (Ghaddar et al. 2014a (link)), Primary antibodies were detected by enhanced chemiluminescence (Roche) after treatment with horseradish-peroxidase-conjugated anti-mouse or anti-rabbit immunoglobulin (Ig) G secondary antibody (Sigma). Signals were detected with CL-Xposure film (Thermo Scientific). Films were scanned and annotated with Photoshop CS5.
Anti hemagglutinin anti ha
Manufactured by Roche
Sourced in Switzerland
Anti-hemagglutinin (anti-HA) is a laboratory reagent used to detect and quantify the presence of hemagglutinin, a viral protein commonly found on the surface of influenza viruses. It functions by binding to the hemagglutinin molecule, allowing for the identification and analysis of influenza viruses.
Automatically generated - may contain errors
Lab products found in correlation
Protran, by PerkinElmer (2 mentions)
Cl xposure film, by Thermo Fisher Scientific (2 mentions)
Anti yeast 3 phosphoglycerate kinase anti pgk, by Thermo Fisher Scientific (2 mentions)
Anti gfp, by Roche (2 mentions)
Enhanced chemiluminescence, by Roche (1 mentions)
Anti ub, by Santa Cruz Biotechnology (1 mentions)
Anti actin sc 47778, by Santa Cruz Biotechnology (1 mentions)
Imagequant las 4000 mini, by Fujifilm (1 mentions)
2 protocols using anti hemagglutinin anti ha
1
SDS-PAGE and Immunoblotting Protocol
2
Western Blot Analysis of Protein Extracts
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Total cell protein extracts were prepared and analyzed by SDS–PAGE as previously described [6 (link)]. Proteins were transferred to either a nitrocellulose membrane (Protran; Perkin Elmer, Waltham, MA, USA) or PVDF (Immobilon, Macherey Nagel, Düren, NW, Germany) and probed with a mouse monoclonal anti-GFP (Roche, Basel, Switzerland), anti-hemagglutinin (anti-HA) (Roche, Basel, Switzerland), anti-yeast 3-phosphoglycerate kinase (anti-PGK; Invitrogen), or anti-actin (sc47778, Santa Cruz Biotechnology, Dallas, TX, USA). Primary antibodies were detected by enhanced chemiluminescence (Roche, Basel, Switzerland, or Millipore, Burlington, MA, USA) after treatment with horseradish-peroxidase-conjugated anti-mouse immunoglobulin (Ig) G secondary antibody (Sigma-Aldrich, St. Louis, MO, USA, or Cell Signalling, Danvers, MA, USA). Signals were detected with CL-Xposure film (Thermo-Fischer Scientific, Waltham, MA, USA) or ImageQuant LAS 4000 mini (FujiFilm, Tokio, Japan). Films were scanned and annotated with FIJI.
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