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Poly lysine 0.01

Manufactured by Merck Group
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Poly-lysine 0.01% is a laboratory product used for cell culture applications. It is a synthetic polymer that promotes cell adhesion to surfaces. The core function of this product is to facilitate the attachment and growth of cells in in-vitro experiments.

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Lab products found in correlation

Lsm 780, by Zeiss (4 mentions) Ab186734, by Abcam (2 mentions) Biotinylated 8 ohdg antibody, by StressMarq Biosciences (2 mentions) Epr15581, by Abcam (2 mentions) Alexa fluor 555 and alexa 488 donkey anti mouse and rabbit conjugated antibodies, by Thermo Fisher Scientific (2 mentions)

Close spelling variants of this product

Poly-L-Lysine 0.01% Poly-L-Lysine 0.01% solution,

2 protocols using poly lysine 0.01

1

Neutrophil Oxidative DNA Damage Imaging

Check if the same lab product or an alternative is used in the 5 most similar protocols
Neutrophils seeded over coverslip chambers (LabTek) pre-coated with poly-lysine 0.01% (Sigma) were fixed in 4% paraformaldehyde overnight and permeabilized in 0.2% Triton for 10 minutes. Oxidized DNA was detected by biotinylated 8-OHdG antibody (StressMarq Biosciences) used at 1 µg/mL and mitochondria (TOM20) was detected using rabbit-anti-human mAb EPR15581 (1:250, ab186734, Abcam), followed by incubation with Alexa Fluor 555 and Alexa 488 donkey anti-mouse and rabbit-conjugated antibodies at 1:250 (Invitrogen) and Hoechst 1:1000. Cells were resuspended in Prolong and visualized with a Zeiss LSM780 laser confocal fluorescence microscope. Images were acquired using the microscope’s associated software.
Lood C., Blanco L.P., Purmalek M.M., Carmona-Rivera C., De Ravin S.S., Smith C.K., Malech H.L., Ledbetter J.A., Elkon K.B, & Kaplan M.J. (2016). Neutrophil extracellular traps enriched in oxidized mitochondrial DNA are interferogenic and contribute to lupus-like disease. Nature medicine, 22(2), 146-153.
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2

Neutrophil Oxidative DNA Damage Imaging

Check if the same lab product or an alternative is used in the 5 most similar protocols
Neutrophils seeded over coverslip chambers (LabTek) pre-coated with poly-lysine 0.01% (Sigma) were fixed in 4% paraformaldehyde overnight and permeabilized in 0.2% Triton for 10 minutes. Oxidized DNA was detected by biotinylated 8-OHdG antibody (StressMarq Biosciences) used at 1 µg/mL and mitochondria (TOM20) was detected using rabbit-anti-human mAb EPR15581 (1:250, ab186734, Abcam), followed by incubation with Alexa Fluor 555 and Alexa 488 donkey anti-mouse and rabbit-conjugated antibodies at 1:250 (Invitrogen) and Hoechst 1:1000. Cells were resuspended in Prolong and visualized with a Zeiss LSM780 laser confocal fluorescence microscope. Images were acquired using the microscope’s associated software.
Lood C., Blanco L.P., Purmalek M.M., Carmona-Rivera C., De Ravin S.S., Smith C.K., Malech H.L., Ledbetter J.A., Elkon K.B, & Kaplan M.J. (2016). Neutrophil extracellular traps enriched in oxidized mitochondrial DNA are interferogenic and contribute to lupus-like disease. Nature medicine, 22(2), 146-153.
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