100 μm nylon filter

Manufactured by BD

Sourced in United States

The 100-μm nylon filter is a laboratory filtration device. It is designed to filter particles and contaminants from liquids. The filter has a pore size of 100 micrometers and is made of nylon material.

Automatically generated - may contain errors

Lab products found in correlation

Liberase, by Roche (5 mentions) Ack lysing buffer, by Lonza (5 mentions) Dnase, by Roche (5 mentions) Ficoll gradient, by Merck Group (2 mentions) Dnase, by Merck Group (1 mentions) Collagenase, by Roche (1 mentions) Ficoll gradient, by BD (1 mentions) Facsaria 2 cell sorter, by BD (1 mentions) Recombinant gm csf, by Thermo Fisher Scientific (1 mentions) Cd11c dcs, by Miltenyi Biotec (1 mentions) Ficoll gradient, by Miltenyi Biotec (1 mentions) Platelet lysate, by Cook Medical (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Trypsin edta, by Merck Group (1 mentions) Culture flask, by Corning (1 mentions) D pbs, by HiMedia (1 mentions) Dulbecco s phosphate buffered saline dpbs, by HiMedia (1 mentions) Dmem f12, by Thermo Fisher Scientific (1 mentions) Gentlemacs dissociator, by Miltenyi Biotec (1 mentions) Hanks balanced salt solution (hbss), by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

100-μm filter (28 citations) Nylon filters (25 citations) Nylon 100 μm mesh filter (6 citations) Nylons (2 citations) Sterile 100 μm nylon filter (2 citations) Sterile 100-μm nylon mesh filter (2 citations)

14 protocols using 100 μm nylon filter

Isolation of Splenocytes and Lung Leukocytes

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Individual spleens were disrupted through a 100 micron nylon filter (Falcon) at the indicated harvest times. Viability of red blood cell depleted splenocytes was determined by ViCell and cells were resuspended at 10x10⁶ viable cells/mL in RPMI 1640 supplemented with 5% FCS, penicillin-streptomycin, 2 mM L-glutamine and 0.1% β-mercaptoethanol (cRPMI-5) prior to use.
Lung leukocytes were isolated from enzyme dispersed lung tissue at the indicated harvest times. Lungs were excised, washed in PBS, minced, and incubated for 45 minutes in RMPI 5% FCS, 1 mg/mL collagenase (Roche Applied Science) and 30 μg/mL DNase (Sigma, St Louis MO) prior to disruption through a 100 micron nylon filter (Falcon). Cells were washed and respuspended in cRPMI-5 and total viable cell counts were determined by ViCell.

Lambert S.L., Aslam S., Stillman E., MacPhail M., Nelson C., Ro B., Sweetwood R., Lei Y.M., Woo J.C, & Tang R.S. (2015). A Novel Respiratory Syncytial Virus (RSV) F Subunit Vaccine Adjuvanted with GLA-SE Elicits Robust Protective TH1-Type Humoral and Cellular Immunity In Rodent Models. PLoS ONE, 10(3), e0119509.

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Murine Tumor and Spleen Cell Isolation

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Mouse tumor samples were minced with scissors prior to incubation with 1.67 U/ml Liberase (Roche) and 0.2 mg/ml DNase (Roche) in RPMI for 30 min at 37 °C. Tumor samples were homogenized by repeated pipetting and filtered through a 100-μm nylon filter (BD Biosciences) in RPMI supplemented with 7.5% FCS to generate single-cell suspensions. Cell suspensions were washed once with complete RPMI and purified on a Ficoll gradient to eliminate dead cells. Cells from mouse spleens were isolated by grinding spleens through 100-μm filters. After red blood cell (RBC) lysis (ACK Lysing Buffer, Lonza) when required, all samples were washed and re-suspended in FACS buffer (PBS/2%FCS).

Holmgaard R.B., Zamarin D., Lesokhin A., Merghoub T, & Wolchok J.D. (2016). Targeting myeloid-derived suppressor cells with colony stimulating factor-1 receptor blockade can reverse immune resistance to immunotherapy in indoleamine 2,3-dioxygenase-expressing tumors. EBioMedicine, 6, 50-58.

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Isolation of Single-Cell Suspensions from Mouse Tumors

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Mouse tumor samples were minced with scissors prior to incubation with 1.67 U/ml Liberase (Roche) and 0.2mg/ml DNase (Roche) in RPMI for 30 min at 37 °C. Tumor samples were homogenized by repeated pipetting and filtered through a 100-μm nylon filter (BD Biosciences) in RPMI supplemented with 7.5% FCS to generate single-cell suspensions. Cell suspensions were washed once with complete RPMI and purified on a Ficoll gradient to eliminate dead cells. Cells from mouse spleens were isolated by grinding spleens through 40-μm filters. After red blood cell (RBC) lysis (ACK Lysing Buffer, Lonza) when required, all samples were washed and re-suspended in FACS buffer (PBS/0.5%albumine) or RPMI depending on further use..

De Henau O., Rausch M., Winkler D., Campesato L.F., Liu C., Cymerman D.H., Budhu S., Ghosh A., Pink M., Tchaicha J., Douglas M., Tibbitts T., Sharma S., Proctor J., Kosmider N., White K., Stern H., Soglia J., Adams J., Palombella V.J., McGovern K., Kutok J.L., Wolchok J.D, & Merghoub T. (2016). Overcoming Resistance to Checkpoint Blockade Therapy by Targeting PI3K-γ in Myeloid Cells. Nature, 539(7629), 443-447.

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Isolation of Pancreatic Islets from Mice

Cited 1 time

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All experiments were performed under guidelines approved by the Florida State University Animal Care and Use Committee, protocol #1235. Islets were isolated by collagenase digestion of the pancreas from male mice (20-40 g) as previously described.^{17 (link)} Briefly, mice were sacrificed by cervical dislocation and a 0.86-mg/mL solution of collagenase P was injected into the pancreas through the pancreatic duct. The pancreas was collected and incubated in 5 mL of the collagenase solution at 37 °C for 8 min with shaking every 2 min. A centrifuge and a 100 μm nylon filter (BD Biosciences, San Jose, CA) were used to filter the islets from the exocrine tissue. After washing with Hanks’ Balanced Salt Solution, the islets were hand-picked under a microscope to ensure high purity of the preparation and cultured in RPMI 1640 medium containing 11 mM glucose, 10% CCS, 100 units/mL penicillin, 100 μg/mL streptomycin, and 10 μg/mL gentamicin at 37 °C in the presence of 5% CO₂. Islets were used within 5 days after isolation.

Wang X, & Roper M.G. (2014). Measurement of DCF fluorescence as a measure of reactive oxygen species in murine islets of Langerhans. Analytical methods : advancing methods and applications, 6(9), 3019-3024.

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Isolation of Mouse Tumor and Spleen Cells

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Mouse tumors were harvested, and minced with scissors before incubation with 2.4 mg/ml collagenase A RPMI-1640 (no fetal bovine serum) at 37°C for 2 h. Tumor samples were washed once with complete RPMI-1640 and purified on a Percoll gradient to eliminate tumor cells. Then the cells were washed again with complete RPMI-1640 and filtered through a 100-μm nylon filter (BD Biosciences) in RPMI-1640 supplemented with 10% FCS to generate single-cell suspensions. Cells from mouse spleens were collected by grinding spleens with frosted glass and filtered through 40-μm filters. After red blood cell (RBC) lysis (ACK Lysing Buffer, Lonza), all cells were washed and re-suspended in FACS buffer (PBS/2% albumin) for further use.

He Y., Du J, & Dong Z. (2020). Myeloid deletion of phosphoinositide-dependent kinase-1 enhances NK cell-mediated antitumor immunity by mediating macrophage polarization. Oncoimmunology, 9(1), 1774281.

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Tumor Cell Dissociation and Isolation

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Tumor samples were minced with scissors before incubation with 1.67 U ml⁻¹ Liberase (Roche) and 0.2 mg ml⁻¹ DNase (Roche) in RPMI for 30 min at 37 °C. Samples were then processed by repeated pipetting and filtered through a 100-μm nylon filter (BD Biosciences) in RPMI to generate single-cell suspensions, which were subsequently washed with complete RPMI and purified on a Ficoll gradient to eliminate dead cells. Single-cell suspensions from spleens were obtained by grinding spleens through 40-μm filters. When required, samples were treated with red blood cell (RBC) lysis buffer (ACK Lysing Buffer, Lonza) and further washed and re-suspended in FACS buffer (PBS/0.5% albumin) before incubation with antibodies.

Campesato L.F., Budhu S., Tchaicha J., Weng C.H., Gigoux M., Cohen I.J., Redmond D., Mangarin L., Pourpe S., Liu C., Zappasodi R., Zamarin D., Cavanaugh J., Castro A.C., Manfredi M.G., McGovern K., Merghoub T, & Wolchok J.D. (2020). Blockade of the AHR restricts a Treg-macrophage suppressive axis induced by L-Kynurenine. Nature Communications, 11, 4011.

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Isolation of Tumor-Infiltrating CD8+ T Cells

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Isolation of tumor-infiltrating lymphoid cells has been described previously (24 (link)). Tumor samples were minced with scissors before incubation with 1.67 U mL⁻¹ Liberase (Roche) and 0.2 mg mL⁻¹ DNase (Roche) in RPMI for 30 minutes at 37°C. Samples were then processed by repeated pipetting and filtered through a 100-μm nylon filter (BD Biosciences) in RPMI to generate single-cell suspensions, which were subsequently washed with complete RPMI and purified on a Ficoll gradient to enrich T cells. Single-cell suspensions from spleens were obtained by grinding spleens through 40-μm filters. When required, samples were treated with red blood cell lysis buffer (ACK Lysing Buffer, Lonza) and further washed and resuspended in FACS buffer (PBS/0.5%albumin) before incubation with antibodies. For the isolation of intratumoral CD8⁺ T cells, shCtrl and shPABPC1L tumors were excised and dissected into single-cell suspensions. Tumor-isolated CD8⁺ T cells were enriched by Ficoll gradient (Sigma-Aldrich) before sorting as (CD45⁺CD3⁺CD8⁺) on a FACSAriaTM II Cell Sorter (BD Biosciences).

Shu G., Chen M., Liao W., Fu L., Lin M., Gui C., Cen J., Lu J., Chen Z., Wei J., Chen W., Wang Y., Zhu J., Zhao T., Liu X., Jing J., Liu G.C., Pan Y., Luo J, & Zhang J. (2024). PABPC1L Induces IDO1 to Promote Tryptophan Metabolism and Immune Suppression in Renal Cell Carcinoma. Cancer Research, 84(10), 1659-1679.

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Generating Tumor-Loaded Dendritic Cells

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Mice were injected subcutaneously with 2 × 10⁶ Flt3L-secreting B16 cells and sacrificed after 2 weeks. Spleens were digested with 1.67 U/ml Liberase (Roche) and 0.2 mg/ml DNase (Roche) in RPMI for 30 min at 37 °C. Samples were filtered through a 100-μm nylon filter (BD Biosciences) and purified on a Ficoll gradient, before positive selection of CD11c + DCs according to the manufacturer's instructions (Miltenyi). DCs were cultured overnight with 10 ng/ml recombinant GM-CSF (PeproTech) and B16 tumor lysate in a 4:1 ratio of dead tumor cells to DCs. After loading, DCs were purified by Ficoll gradient (Sigma).

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Isolation and Expansion of Ad-MSCs

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Adipose tissue samples were purchased from Caltag MedSystem (Buckingham, UK). The project was approved by the Ethics Committee of Hospital Fundación Jiménez Díaz (Madrid, Spain). Adipose tissue was disaggregated and digested with collagenase A (Serva, Heidelberg, Germany) at a final concentration of 2 mg/ml for 4 h at 37°C. Digested samples were filtered through 100-μm nylon filters (BD Bioscience, NJ, USA) and centrifuged for 10 min. The cell pellet was re-suspended in Minimum Essential Medium α (ɑ-MEM; Gibco/Life Technologies/Thermo Fisher Scientific, Waltham, USA) supplemented with 5% platelet lysate (Cook Medical, IN, USA), 1% penicillin/streptomycin (Gibco/Life Technologies/Thermo Fisher Scientific, Waltham, USA), and 1 ng/ml human basic fibroblast growth factor (bFGF; Peprotech, NJ, USA). Cells were seeded at a concentration of 10,000 cells/cm² in culture flasks (Corning, NY, USA) and cultured at 37°C. For the expansion of adipose tissue derived-MSCs (Ad-MSCs), the cell medium was changed every 2–4 days and adherent cells were serially passaged using 0.25% trypsin/EDTA (Sigma Aldrich, St. Louis, MO, USA) upon reaching near confluence (70%–90%).

Hervás-Salcedo R., Fernández-García M., Hernando-Rodríguez M., Suárez-Cabrera C., Bueren J.A, & Yáñez R.M. (2023). Improved efficacy of mesenchymal stromal cells stably expressing CXCR4 and IL-10 in a xenogeneic graft versus host disease mouse model. Frontiers in Immunology, 14, 1062086.

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Isolation of Medaka Granulosa Cells

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Preovulatory follicles were collected from the ovaries of spawning female medaka 3 h before ovulation as previously described (Ogiwara et al., 2013) . In brief, the follicle layers consisting of both granulosa cells and theca cells were separated from the oocytes using forceps. After washing three times with PBS, the tissues were placed in phosphate-buffered saline (PBS) containing 1 mM EDTA and 0.25% trypsin followed by gentle rotation for 30 min at room temperature. Treated samples were collected by centrifugation at 2000 rpm for 3 min. After three washes with 90% medium 199 solution, the precipitates containing the granulosa and theca cells were suspended in the same medium and filtered with 100-μm nylon filters (BD Bioscience, Bedford, MA). The resultant filtrates were cultured in medium containing 50 μM gentamycin and 5% carp serum. After culturing for 48 h, unattached cells were removed by gentle washing with PBS, and the cells that remained attached to the dish were collected. The cells obtained in this manner were granulosa cells derived from follicles that were predicted to ovulate (Kato et al., 2010) . Total RNA for RT-PCR analysis was isolated from the collected cells.

Hagiwara A., Ogiwara K, & Takahashi T. (2016). Expression of Membrane Progestin Receptors (mPRs) in Granulosa Cells of Medaka Preovulatory Follicles. Zoological science, 33(1).

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