Crystalline pyocyanin was purchased from Cayman or prepared by photolysis of 2 mM solution of phenazine methosulfate in 20 mM TRIS buffer, pH 7.4, as reported previously (Cheluvappa, 2014 (link)); the crude synthetic pyocyanin was recrystallized from the TRIS buffer and judged essentially pure by its UV–Vis and ESI mass-spectra. Reduced glutathione (GSH) was obtained from Sigma. The GSH and GSSG content in glutathione stock solutions was determined fluorimetrically with o-phthaladehyde (Senft et al., 2000 (link)). In-house nanopure water was generated by double distillation and used in all experiments. Stock HEPES buffer (200 mM) was treated with 5% (v/v) Chelex resin (BioRad) by stirring overnight. All other commercial reagents, solvents and media were used without any further purification.
Crystalline pyocyanin
Manufactured by Cayman Chemical
Crystalline pyocyanin is a laboratory product offered by Cayman Chemical. Pyocyanin is a blue-green pigment produced by the bacterium Pseudomonas aeruginosa. The crystalline form of pyocyanin is used for research purposes.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using crystalline pyocyanin
1
Purification and Characterization of Pyocyanin
2
Antibiotic and Phenazine Compound Preparation
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Non-pharmaceutical-grade antibiotics included colistin sulfate (∼20,000 U/mg; Chem-Impex), colistin sodium methanesulfonate (CMS) (from Bacillus colistinus ; Fluka), and polymyxin B sulfate (∼8,000 U/mg; Research Products International). Stock solutions of CMS were kept frozen until use. The reagent-grade phenazines 1-hydroxyphenazine (1-HP), phenazine-1-carboxylic acid (PCA), and phenazine-1-carboxamide (PCN) were obtained from ArkPharm and were used without further purification. Crystalline pyocyanin was purchased from Cayman or prepared by photolysis of a 2 mM solution of phenazine methosulfate in 20 mM HEPES buffer (pH 7.4), as reported previously (32 (link)). In-house nanopure water was generated by double distillation and used in all experiments. Antioxidants employed in this study included N-acetyl-l -cysteine (NAC), tyrphostin AG490, chlorogenic acid (ChlA), deferasirox (Dfx), the cell-permeable catalase/SOD mimic EUK134, o-phenanthroline (o-Phen), and resveratrol (Resv).
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