Erastin was obtained from the Duke University Small Molecule Synthesis Facility. The following antibodies, their catalog numbers, sources and diltuionswere indicated below: YAP/TAZ (#8418, Cell Signaling Technology, 1:1000), βa-tubulin (#86298, Cell Signaling Technology, 1:2000), vinculin (sc-73614, Santa Cruz, 1:2000), V5 tag (#13202, Cell Signaling Technology, 1:2000), H3 (#4499, Cell Signaling Technology, 1:2000), GAPDH (sc-25778, Santa Cruz, 1:2000), ANGPTL4 (#40–9800, ThermoFisher Scientific, 1:1000), NOX2 (sc-130543, Santa Cruz, 1:1000), anti-rabbit IgG, horseradish peroxidase (HRP)-linked antibody (#7074, Cell Signaling Technology, 1:2000–1:4000) and anti-mouse IgG, HRP-linked Antibody (#7072, Cell Signaling Technology, 1:2000–1:4000). Plasmids were obtained from Addgene (TAZS89A #52084; ANGPTL4-V5 #102446). The NOX2 inhibitor, gp91 ds-tat, was purchased from Eurogentec (cat #: AS-63818) and recombinant human ANGPTL4 protein was purchased from Novus Biologicals (4487-AN). VAS2870 (Calbiochem-492000), GKT136901 (Calbiochem-534032), Z-VAD-FMK (SML0583), ferrostatin-1 (SML0583) and liproxstatin-1 (SML1414) were purchased from Sigma.
Tazs89a
Manufactured by Addgene
TAZS89A is a piece of lab equipment. It is designed for use in scientific research and experiments.
Automatically generated - may contain errors
Lab products found in correlation
Angptl4, by Thermo Fisher Scientific (1 mentions)
Z vad fmk, by Merck Group (1 mentions)
Ferrostatin 1, by Merck Group (1 mentions)
Liproxstatin 1, by Merck Group (1 mentions)
Gp91 ds tat, by Eurogentec (1 mentions)
Vinculin, by Santa Cruz Biotechnology (1 mentions)
Yap taz, by Cell Signaling Technology (1 mentions)
V5 tag, by Cell Signaling Technology (1 mentions)
Gapdh, by Santa Cruz Biotechnology (1 mentions)
Anti rabbit igg na934v, by GE Healthcare (1 mentions)
L glutamine, by Thermo Fisher Scientific (1 mentions)
Dapi d9542, by Merck Group (1 mentions)
Anti β actin a5441, by Merck Group (1 mentions)
Control sirna sc 37007, by Santa Cruz Biotechnology (1 mentions)
Biocoat cell inserts, by BD (1 mentions)
Yap1 sirna s102662954, by Qiagen (1 mentions)
Cell lysis buffer 9803, by Cell Signaling Technology (1 mentions)
Taz 4883, by Cell Signaling Technology (1 mentions)
Peroxidase conjugated anti mouse igg nxa931, by GE Healthcare (1 mentions)
Matrigel 354234, by BD (1 mentions)
2 protocols using tazs89a
1
Molecular Mechanisms of Ferroptosis Regulation
2
Investigating the Role of YAP1 and TAZ in Cell Signaling
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Sodium pyruvate (11360-070), DMEM (11960-077), 0.25% trypsin-EDTA (25200-056) and L-Glutamine (25030-164) were purchased from Gibco (Grand Island, NY). Heat-inactivated fetal calf serum (FCS, F4135), lactacystin (L6785) and anti-β-actin (A5441) were purchased from Sigma (St. Louis, Mo). Cell lysis Buffer (9803) was purchased from Cell Signaling Technology (Danvers, MA). YAP1 (4912), YAP1-P (13008), Histone 3 (#9717) and TAZ (4883) antibodies were purchased from Cell Signaling Technologies. Anti-p63 (sc-8431), anti-ubiquitin (sc-9133) and anti-TAZ-P (17610) were purchased from Santa Cruz. Anti-pan-TEAD (ab1791) was purchased from Abcam (Cambridge, MA). Peroxidase-conjugated anti-mouse IgG (NXA931) and anti-rabbit IgG (NA934V) were obtained from GE healthcare (Buckinghamshire, UK). DAPI (D9542) was purchased from Sigma Chemicals (St. Louis, MO). p63- (sc-36161), TAZ- (sc-38568A) and control-siRNA (sc-37007) were purchased from Santa Cruz (Dallas, TX). YAP1-siRNA (S102662954) was purchased from Qiagen (Valencia, CA). Matrigel (354234) and BD Biocoat cell inserts (353097) were purchased from BD Biosciences (Franklin Lakes, NJ). Sulforaphane (S8044) was purchased from LKT Laboratories INC (St. Paul, MN). YAP(S127A) (Addgene plasmid # 27370) and TAZ(S89A) (Addgene plasmid # 32840) were from Kunliang Guan [41 (link)].
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