Ab41718

Manufactured by Abcam

Ab41718 is a monoclonal antibody targeting an undisclosed antigen. The core function of this product is to specifically bind to its target. Further details about its intended use are not available.

Automatically generated - may contain errors

Lab products found in correlation

Hela s3, by American Type Culture Collection (1 mentions) Anti h3k14cr ptm 535, by PTM Biolabs (1 mentions) Anti flag tag, by Merck Group (1 mentions) Bioruptor, by Diagenode (1 mentions) Red chip kit, by Diagenode (1 mentions)

2 protocols using ab41718

Immunodetection of Chromatin Modifications

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For western blot: anti-MOZ (ab41718, Abcam); anti-DPF2 (A303-596A-1, Bethyl); anti-H3K14cr (PTM-535, PTM Biolabs)
For colocalization: anti-FLAG tag (F1804, Sigma); anti-H3K14cr (PTM-535, PTM Biolabs); Cy5-conjugated goat anti-rabbit (111-175-144, Jackson ImmunoResearch); goat anti-mouse (A-11029, Life Technologies)
Cell lines: HeLa S3 from ATCC #CCL-2.2; HeLa from ATCC #CCL-2; 293T from ATCC #CRL-3216; K562 from ATCC #CCL-243. All cell lines were no mycoplasma contamination tested by PCR.

Xiong X., Panchenko T., Yang S., Zhao S., Yan P., Zhang W., Xie W., Li Y., Zhao Y., Allis C.D, & Li H. (2016). Selective Recognition of Histone Crotonylation by Double PHD Fingers of MOZ and DPF2. Nature chemical biology, 12(12), 1111-1118.

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ChIP-qPCR Assay for Histone Modifications

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Chromatin immunoprecipitation was performed using the Red ChIP Kit (Diagenode, www.diagenode.com) following the instructions of the manufacturer. Crosslinked cells were sonicated for 15 cycles (30s on/30s off) with the Bioruptor (Diagenode, www.diagenode.com). Antibodies used were RNA Polymerase (H-224 from Santa Cruz Biotechnology, www.scbt.com) and anti-HAT MYST3 antibody (Ab41718 from Abcam, www.abcam.com). Ten million cells were used for each immunoprecipitation with the anti-Moz antibody. Eluted chromatin was quantified by qualitative PCR (qPCR). Data for ChIP were obtained by subtracting IgG control values to the corresponding antibody values. Graphs represent fold increase over control IgG.

Perez-Campo F.M., Costa G., Lie-a-Ling M., Stifani S., Kouskoff V, & Lacaud G. (2014). MOZ-Mediated Repression of p16INK4a Is Critical for the Self-Renewal of Neural and Hematopoietic Stem Cells. Stem Cells (Dayton, Ohio), 32(6), 1591-1601.

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