Samples were thawed and injected into a system of ESA 584 pumps (ESA, Chelmsfor, MA) with a pre-column filter (Synergi Max-RP4u Security Guard, 150×4.6mm, Phenomenex Inc., Torrance CA, USA) and Max-RP cartridges (Phenomenex), as we performed previously (Masini et al., 2004 (link)). Mobile phase was delivered at 1mL/min and contained 100mM sodium phosphate monobasic (Fisher, Pittsburgh PA, USA), 0.1mM EDTA (Sigma), 0.25mM octanesulfonic acid (Sigma), and 5% acetonitrile (JT Baker). Samples and standards were injected at 20μL using ESA 542 at 4°C. Peaks were detected over 30 min using ESA CoulArray (−150 and 200 mV on the initial and final electrodes). Position and height of dopamine peaks were compared to standards (Sigma; diluted in aCSF). Standards were run in duplicate/12 samples. Dopamine detection limit was 13.7 nmol/mL. Peak chromatogram area was integrated and analyzed by CoulArray 3.05.
Octanesulfonic acid
Manufactured by Merck Group
Sourced in United States
Octanesulfonic acid is a chemical compound used in various laboratory applications. It is a straight-chain alkanesulfonic acid with the molecular formula C8H17SO3H. The compound is commonly used as a buffer, ion-pairing agent, and in analytical techniques such as high-performance liquid chromatography (HPLC).
Automatically generated - may contain errors
Lab products found in correlation
Synergi max rp4u security guard, by Phenomenex (2 mentions)
Max rp cartridges, by Phenomenex (2 mentions)
Ethylene diamine tetraacetic acid (edta), by Merck Group (2 mentions)
Acetonitrile, by Merck Group (2 mentions)
Acetic acid, by Merck Group (2 mentions)
Sodium phosphate monobasic, by Thermo Fisher Scientific (1 mentions)
Acetonitrile, by Avantor (1 mentions)
1100 series, by Agilent Technologies (1 mentions)
Hypersil ods, by Thermo Fisher Scientific (1 mentions)
Sodium phosphate monobasic, by Merck Group (1 mentions)
Bd1047, by Bio-Techne (1 mentions)
Phosphate buffered saline (pbs), by Merck Group (1 mentions)
Arium water purification system, by Sartorius (1 mentions)
Plga lactide glycolide 65 35, by Merck Group (1 mentions)
Polyvinyl alcohol (pva), by Merck Group (1 mentions)
Sodium acetate, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
6 protocols using octanesulfonic acid
1
Dopamine Quantification via HPLC-ECD
2
Histamine Quantification in Dialysates
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Histamine contents in the dialysates were determined by HPLC-fluorometry (Munari et al., 2013 (link)). In brief, the column (Hypersil ODS, 3 µm, 2.1×100 mm; Thermo Fisher Scientific, Waltham, MA) was eluted with 0.25 M potassium dihydrogen phosphate containing 5% octanesulfonic acid (Sigma-Aldrich, St. Louis, MO) at a flow rate of 0.4 mL/min. The eluate from the column was mixed first with 0.1% o-phthalaldehyde solution at a flow rate of 0.1 mL/min and then to a solution containing 4 M sodium hydroxide and 0.2 M boric acid (flow rate, 0.137 mL/min) to adjust the reaction mixture to pH 12.5. The reaction took place at 45°C. Then 17% orthophosphoric acid was added to the solution (flow rate, 0.137 mL/min) to reach a final reaction mixture at pH 3. The fluorescent intensity was measured with a spectrofluorometer (series 1100; Agilent, Waldbronn, Germany) at 450 nm with excitation at 360 nm. The sensitivity limit was 10 fmol and the signal/noise ratio was >3. Histamine levels in the dialysate samples were calculated as fmol/30 min and were not corrected for probe recovery (about 40%).
3
Quantifying Histamine Levels by HPLC
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To prevent degradation of histamine (HA), 1.5 µL of 5mM HCl was added to each sample. The dialysates were kept at -80°C until analysis. HA contents in the dialysates were determined by HPLC-fluorometry (Cenni et al., 2006 (link)). In brief, the column (Hypersil ODS, 3 µm, 2.1×100mm; Thermo Fisher Scientific, Waltham, MA) was eluted with 0.25M potassium dihydrogen phosphate containing 5% octanesulfonic acid (Sigma-Aldrich, St. Louis, MO) at a flow rate of 0.4mL/min. The eluate from the column was mixed first with 0.1% o-phthalaldehyde solution at a flow rate of 0.1mL/min and then to a solution containing 4M sodium hydroxide and 0.2M boric acid (flow rate, 0.137mL/min) to adjust the reaction mixture to pH 12.5. The reaction took place at 45°C. Then 17% orthophosphoric acid was added to the solution (flow rate, 0.137mL/min) to reach a final reaction mixture at pH 3. The fluorescent intensity was measured with a spectrofluorometer (series 1100; Agilent, Waldbronn, Germany) at 450nm with excitation at 360nm. The sensitivity limit was 10fmol and the signal/noise ratio was higher than 3. HA levels in the dialysate samples were calculated as fmol/30min. In α-FMHis–treated mice, HA levels were below the detection sensitivity limits of the apparatus.
4
HPLC Analysis of Dopamine in Dialysates
5
Neurotoxin-Induced Parkinson's Model
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The following chemicals were used: afobazole (5-ethoxy-2-[2-(morpholino)-ethylthio]benzimidazole dihydrochloride) (FSBI Research State Zakusov Institute of Pharmacology, Moscow, Russia), PRE-084, BD-1047 (Tocris Bioscience, Bristol, UK), 6-hydroxydopamine hydrochloride (6-OHDA), ascorbic acid, NaCl, sucrose, paraformaldehyde (PFA), polyclonal antibodies against tyrosine hydroxylase T8700, secondary antibodies conjugated with CF488 fluorochrome SAB4600045, FluoroShield, 4′,6-diamidino-2-phenylindole, Triton X-100, chloral hydrate, 3,4-dihydroxybenzylamine hydrobromide (DHBA), dopamine (DA), 3,4-dihydroxyphenylacetic acid (DOPAC), homovanillic acid (HVA), KH2PO4, H3PO4, HClO4, citric acid, ethylenediaminetetraacetic acid disodium salt dehydrate (EDTA-Na2), octanesulfonic acid, acetonitrile, phosphate-buffered saline (PBS) (Sigma-Aldrich, St. Louis, MO, USA), and Tissue Tek O.C.T. medium (Sakura, Osaka, Japan).
6
HPLC Characterization of BNIPDaoct
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Sodium acetate and octanesulfonic acid were purchased from Sigma-Aldrich (St Louis, MO, USA). Acetonitrile (LiChrosolv HPLC grade), dimethyl sulfoxide (DMSO) and acetic acid were obtained from Merck (Darmstadt, Germany). Water from Arium water purification system (resistivity > 18 MΩ cm, Sartorius, Goettingen, Germany) was used for the preparation of solutions. Aqueous buffer (acetic acid/acetate 0.10 mol L -1 , pH 4.5, 0.010 mol L -1 octanesulfonic acid) was filtered through a 0.22 μm Millipore GVWP filter. Prior to use, the mobile phase was degassed in an ultrasonic bath for 15 min. PLGA (lactide:glycolide [65:35], molecular weight: 40,000-75,000 Da) and poly(vinyl alcohol) (PVA; 87-89% hydrolyzed, molecular weight: 13,000-23,000 Da) were acquired from Sigma-Aldrich. BNIPDaoct, represented in Fig. S1, was synthesized as described previously [4] .
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