Aperio lv1 ivd equipment

The Claudin-1 protein expression was determined in descending colon tissue. The resected tissue was fixed immediately with 10% formaldehyde in 1X phosphate buffer saline (PBS), processed and embedded in paraffin. Paraffin-embedded samples were cut, and sections (3 µm) were treated with the Novolink polymer detection system according to the manufacturer’s instructions. Antigen retrieval was performed with EDTA buffer (1 mM EDTA, 0.05% Tween 20, and pH 8.0) for 40 min.
Sections were incubated overnight at 4 °C with a mouse monoclonal primary antibody (sc-166338, Santa Cruz Biotechnology, Danvers, MA, USA) at a 1:250 dilution. Adipose tissue was used as a negative control. Finally, the chromogen working solution and hematoxylin were used to perform the detection and counterstain, respectively. The claudin-1 expression was documented using the Aperio LV1 IVD equipment and Aperio imagescope software (Leica Biosystem, Chicago, IL, USA). The staining intensity was scored as follows: 0 = negative, 1 = weak, 2 = moderate, and 3 = strong [66 (link)].

The Claudin-1 protein expression was determined in the ascending and descending colon tissue. The resected tissue was fixed immediately with 10% formaldehyde in 1X phosphate buffer saline (PBS), processed, and embedded in paraffin. The paraffin-embedded samples were cut and the sections (3-µm) were treated with the Novolink^® polymer detection system (Leica Biosystems, Buffalo Grove, IL, USA), according to the manufacturer’s instructions. The antigen retrieval was performed with an EDTA buffer (1 mM EDTA, 0.05% Tween 20, and pH 9.0) for 40 min.
The sections were incubated overnight at 4 °C with a mouse monoclonal primary antibody (sc-166338; Santa Cruz Biotechnology, Danvers, MA, USA) at a 1:250 dilution. The adipose tissue was used as the negative control. Finally, the chromogen working solution and hematoxylin were employed to perform the detection and the counterstain, respectively. The Claudin-1 expression was documented using Aperio LV1 IVD equipment and Aperio image scope software (Leica Biosystems, Buffalo Grove, IL, USA). The staining intensity was scored as follows: 0 = negative; 1 = weak; 2 = moderate, and 3 = strong.