Cell cycle progression was examined using cell cycle analysis kits (cat no. BL114A, Biosharp Life Sciences). SiHa and CaSki cells (5×105 cells) were collected by centrifugation (106 × g for 5 min) at 4°C and fixed with ice-cold 70% ethanol for 2 h at 4°C. The cell pellets were washed with PBS twice and the staining buffer, PI (20×) and RNase A (50×) (cat no. BL114A, Biosharp Life Sciences) were added. After incubation at 37°C for 30 min, the cells were analyzed by a flow cytometer (NovoCyte 2040R; ACEA Bioscience, Inc.; Agilent Technologies, Inc.) using the NovoExpress software (version 1.4.1; ACEA Bioscience, Inc.; Agilent Technologies, Inc.).
Bl114a
Manufactured by Biosharp
The BL114A is a laboratory equipment device designed for general use in research and scientific settings. It serves as a core functional component without further interpretations on its intended applications.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using bl114a
1
Cell Cycle Analysis of SiHa and CaSki Cells
2
Apoptosis and Cell Cycle Analysis
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For cell apoptosis analysis, 400 μl binding buffer was added to 106 cells/ml transfected gastric cancer cells. After adding 5 µl of Annexin V and 5 μl PI (BD, 556547, USA) each, samples were incubated for 15 min at 25 ℃ in the dark. The cells were analyzed via flow cytometry (Becton Dickinson, USA).
For the cell cycle assay, 106 cells/ml of transfected were fixed overnight at 4 °C with pre-cooled 70% ethanol. Next, 500 μl staining buffer, 25 μl PI (20 ×) staining solution, and 10 μl RNase A (50 ×) (Biosharp, BL114A) were added. After incubation at 37 °C for 30 min, cells were analyzed via flow cytometry.
For the cell cycle assay, 106 cells/ml of transfected were fixed overnight at 4 °C with pre-cooled 70% ethanol. Next, 500 μl staining buffer, 25 μl PI (20 ×) staining solution, and 10 μl RNase A (50 ×) (Biosharp, BL114A) were added. After incubation at 37 °C for 30 min, cells were analyzed via flow cytometry.
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