Mydrin p ophthalmic solution

Electroretinograms were recorded as described previously with modification (29 (link)). Briefly, mice were dark adapted overnight and anesthetized with a combination anesthetic (0.3 mg/kg medetomidine, 4.0 mg/kg midazolam, and 5.0 mg/kg butorphanol) by i.p. injection. Pupils were dilated with Mydrin-P ophthalmic solution (1319810Q1053, Santen Pharmaceutical) under dim red light, and 0.1% hyalein ophthalmic solution (1319720Q3078, Santen Pharmaceutical) was used to moisten the cornea. Gold electrodes were placed on corneal surfaces, the reference electrode was placed intraorally, and the ground electrode was inserted intra-anally. Body temperature was maintained at approximately 37°C with a heating pad. STR recordings were performed simultaneously in both eyes at an intensity range of –6 to –3.5 log scot.cd s/m² using a Ganzfeld bowl (LS-100, Mayo). The responses were band-pass filtered from 0.3–50 Hz and averaged over 30 trials (PuREC PC-100, Mayo). The amplitudes of positive STRs were measured from baseline.

Live imaging of retinal sections was performed using OCT on 2- to 6-month-old Mettl23-KI and -KI mice under a combination anesthetic (0.3 mg/kg medetomidine, 4.0 mg/kg midazolam, and 5.0 mg/kg butorphanol) injected i.p., as described previously (64 (link)). Pupils were dilated with Mydrin-P ophthalmic solution (1319810Q1053, Santen Pharmaceutical). Fundus examinations and OCT studies were performed using a Micron IV (Phoenix Technology Group). Structural analysis of OCT data was performed using INSIGHT software (Phoenix Technology Group).

An infrared photorefractor (Steinbeis Transfer Center, Germany) was used to measure the refractive state^{6 (link),63} . The mouse eye was instilled by Tropicamide, Penylephrine Hydrochloride solution (Mydrin-P ophthalmic solution, Santen Pharmaceutical Co., Ltd) 5 minutes before the measurement to ensure mydriasis and cycloplegia. After the injection of MMB to induce general anesthesia, 99 measurements were taken along the optical axis for one eye and the averages were recorded as the refraction. The way to obtain stable results was described precisely in the results.
Another infrared photorefractor (Steinbeis Transfer Center, Germany) was used to measure the corneal curvature according to previous reports⁶³ . After the injection of MMB to induce general anesthesia, eight infrared LEDs placed as a disc lit the mouse cornea, and the reflected light was detected with an infrared camera. The diameter of the circle made by the reflected light on the mouse cornea was taken as the corneal curvature.

An infrared photorefractor (Steinbeis Transfer Center, Stuttgart, Germany) (Schaeffel, 2008 (link)) was used to measure the refractive state. To decrease movement of the mice and measure the REs accurately, they were lightly anesthetized with an intraperitoneal injection of ketamine (Vedno, St. Joseph, MO, United States) and xylazine (Akorn, Decatur, IL, United States) [10 and 1 mg (kg body weight) ^–1, respectively]. The eyes were instilled with tropicamide, phenylephrine hydrochloride solution (Mydrin-P ophthalmic solution; Santen Pharmaceutical Co., Ltd., Osaka, Japan), 5 min before the measurement to ensure mydriasis and cycloplegia. To determine refraction, 20 measurements were taken along the optical axis for each eye, and the averages were calculated. The results were confirmed by streak retinoscopy refraction. If a difference of refractive power greater than 5 diopters (D) between the two methods was observed, the animal was excluded.
The infrared photorefractor was also used to measure the corneal curvature (Schaeffel, 2008 (link)), which was determined by the diameter of the circle made by the reflected light on the mouse cornea. The aim was to rule out corneal damage in the lid-suture mouse model.