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Filmarray respiratory panel 2

Manufactured by bioMérieux
Sourced in Japan

The FilmArray Respiratory Panel 2.1 is a molecular diagnostic test that simultaneously detects and identifies multiple respiratory pathogens, including viruses and bacteria, from a single patient sample. The panel is designed to provide rapid and comprehensive results to aid in the diagnosis of respiratory infections.

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2 protocols using filmarray respiratory panel 2

1

SARS-CoV-2 RT-qPCR Screening and Co-infections

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SARS-CoV-2 RT-qPCR was performed at officially approved laboratories, including the University of Toyama, Toyama Institute of Health, and external private laboratories. The RT-qPCR varied by laboratory. Cases of RNAemia were screened (31 (link)), with the results used as the demographic background. When the remaining respiratory specimens were available, co-infecting microorganisms were screened using the FilmArray Respiratory Panel 2.1 (bioMérieux Japan, Tokyo, Japan), according to the manufacturer’s instructions.
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2

Multimodal SARS-CoV-2 Detection Protocols

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Nasopharyngeal swab samples were collected using cotton swabs and placed in viral transport media (Copan Diagnostics, Murrieta, CA, USA). Multiple molecular diagnostic testing platforms, including SARS-CoV-2 quantitative reverse transcriptase-PCR in accordance with the protocol developed by the National Institute of Infectious Diseases in Japan (Shirato et al., 2020 (link)), FilmArray Respiratory Panel 2.1 with the FilmArray Torch system (bioMérieux, Marcy-l'Etoile, France) (Hirotsu et al., 2020a (link)), Xpert Xpress SARS-CoV-2 test using Cepheid GeneXpert (Cepheid, Sunnyvale, CA, USA) (Hirotsu et al., 2022 (link)), and Lumipulse antigen test with the LUMIPULSE G600II system (Fujirebio, Inc., Tokyo, Japan) were used to identify positive samples for this study (Hirotsu et al., 2021 (link); Hirotsu et al., 2020b ).
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