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2 3 butanedione 2 monoxime bdm

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2,3-butanedione 2-monoxime (BDM) is a chemical compound used in various laboratory applications. It functions as a reversible inhibitor of actomyosin ATPase activity, making it a useful tool in the study of muscle contraction and cell motility mechanisms.

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Lab products found in correlation

Etomoxir, by Merck Group (2 mentions) Physcion, by Merck Group (2 mentions) Cb 839, by Aurogene (2 mentions)

Spelling variants of this product

2,3-butanedione monoxime (42 citations) 2,3-butanedione (25 citations) BDM (2,3-butanedione monoxime) (15 citations) 2,3-butanedione 2-monoxime (3 citations) Butanedione monoxime (3 citations) 2,3-BDM (3 citations)

3 protocols using 2 3 butanedione 2 monoxime bdm

1

Zebrafish Cardiac Contractility Modulation

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For the vMCs coverage analysis, zebrafish embryos were treated with the following drugs: 6-AN (Sigma), Physcion (Sigma), CB-839 (Aurogene #S-7655), Etomoxir (Sigma) and AZ67 (TOCRIS #5742) from 48 hpf (hours post fertilization) onward. The myosin ATPase inhibitor 2,3-butanedione 2-monoxime (BDM) (Sigma) was used to block cardiac contraction in the developing embryo as previously published. BDM was dissolved at 4 mM in fish water embryo media and added after the establishing of the embryonic circulation at 30 hpf until 3 dpf (days post fertilization). R5P was injected at 500 mM in once cell stage zebrafish embryos.
Facchinello N., Astone M., Audano M., Oberkersch R.E., Spizzotin M., Calura E., Marques M., Crisan M., Mitro N, & Santoro M.M. (2022). Oxidative Pentose Phosphate Pathway Controls Vascular Mural Cell Coverage by Regulating Extracellular Matrix Composition. Nature metabolism, 4(1), 123-140.
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2

Zebrafish Cardiac Contractility Modulation

Check if the same lab product or an alternative is used in the 5 most similar protocols
For the vMCs coverage analysis, zebrafish embryos were treated with the following drugs: 6-AN (Sigma), Physcion (Sigma), CB-839 (Aurogene #S-7655), Etomoxir (Sigma) and AZ67 (TOCRIS #5742) from 48 hpf (hours post fertilization) onward. The myosin ATPase inhibitor 2,3-butanedione 2-monoxime (BDM) (Sigma) was used to block cardiac contraction in the developing embryo as previously published. BDM was dissolved at 4 mM in fish water embryo media and added after the establishing of the embryonic circulation at 30 hpf until 3 dpf (days post fertilization). R5P was injected at 500 mM in once cell stage zebrafish embryos.
Facchinello N., Astone M., Audano M., Oberkersch R.E., Spizzotin M., Calura E., Marques M., Crisan M., Mitro N, & Santoro M.M. (2022). Oxidative Pentose Phosphate Pathway Controls Vascular Mural Cell Coverage by Regulating Extracellular Matrix Composition. Nature metabolism, 4(1), 123-140.
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3

Zebrafish Cardiac Troponin 2 Knockdown

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All morpholino oligonucleotides (MOs; Gene Tools, LLC) were used as previously reported [48 (link),49 (link)]. The TNNT2 MO (5’ CAT GTT TGC TCT GAT CTG ACA CGC A 3') was designed to block the translational start site of zebrafish cardiac troponin 2 (tnnt2; silent heart, sih). The standard Gene Tools Control MO (5’-CCT CTT ACC TCA GTT ACA ATT TAT A-3’) was used to control for non-specific responses. One-cell embryos were injected with 2 ng of MO. 2,3-Butanedione 2-monoxime (BDM, Sigma Aldrich) was used at a final concentration of 10 mM embryo water.
Plavicki J.S., Hofsteen P., Yue M.S., Lanham K.A., Peterson R.E, & Heideman W. (2014). Multiple modes of proepicardial cell migration require heartbeat. BMC Developmental Biology, 14, 18.
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