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2 protocols using synergy ultrapure water

1

Synthesis and Functionalization of Gold Nanoparticles

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Sodium borohydride (NaBH4), hexadecyltrimethylammonium bromide (CTAB), HAuCl4 solution 200 mg/dL in deionized water, ascorbic acid (AA), hexadecyltrimethylammonium chloride (CTAC), polyvinylpyrrolidone Mw ~55,000 (PVP), methanol (MeOH), 1,3,3,1’,3’,3’,-hexamethyl-2,2’-indotricarbocyanine iodide (HITC), tetradecanol (TD), phosphate buffer saline (PBS), tris-EDTA buffer solution (TE), tween 20, sodium chloride (NaCl), hydrochloric acid (HCl) were purchased from Sigma-Aldrich. Sodium citrate dihydrate was purchased from BDH. Methoxy polyethylene glycol thiol Mw 5000 (mPEG-SH) was purchased from Nanocs. Magnetic beads (Dynabeads MyOne Streptavidin C1, 1 μm diameter) were purchased from Life Technologies. All DNA sequences (Table S1) were synthesized by Integrated DNA Technologies (IDT, Coralville, IA). Millipore Synergy ultrapure water (DI) of resistivity = 18.2 MΩ cm was used in all aqueous solutions.
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2

Gold Nanoparticle Synthesis Protocol

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Sodium borohydride (NaBH4), Au chloride solution (HAuCl4) 200 mg/dL in deionized water, ascorbic acid (AA), hexadecyltrimethylammonium chloride (CTAC) solution 25 wt% in water, polyvinylpyrrolidone Mw ~55,000 (PVP), ethanol (EtOH), 1,3,3,1′,3′,3′,-hexamethyl-2,2′-indotricarbocyanine iodide (HITC), phosphate buffer saline (PBS), tris-EDTA buffer solution (TE), tween 20, sodium chloride (NaCl), hydrochloric acid (HCl) were purchased from Sigma-Aldrich. Sodium citrate dihydrate was purchased from BDH. Methoxy polyethylene glycol thiol Mw 5000 (mPEG-SH) was purchased from Nanocs. Magnetic beads were purchased from Life Technologies. All DNA sequences were synthesized by Integrated DNA Technologies (IDT, Coralville, IA). Millipore Synergy ultrapure water (DI) of resistivity = 18.2 MΩ cm was used in all nanoparticle synthesis solutions. Nuclease-free water was used in all experiments relating to RNA and DNA.
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