Stat3 inhibitor

Manufactured by Bio-Techne

The Stat3 inhibitor is a laboratory reagent designed to inhibit the activity of the Stat3 (Signal Transducer and Activator of Transcription 3) protein. Stat3 is a transcription factor involved in cellular processes such as cell growth, differentiation, and survival. The Stat3 inhibitor can be utilized in research applications to study the role of Stat3 signaling in various biological systems.

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Lab products found in correlation

Mifepristone, by Merck Group (1 mentions) Pd169316, by Merck Group (1 mentions) Jsi124, by Bio-Techne (1 mentions) Ly294002, by Bio-Techne (1 mentions) U0126, by Merck Group (1 mentions) Fluorescent plate reader, by Thermo Fisher Scientific (1 mentions) Pka inhibitor h89, by Merck Group (1 mentions)

2 protocols using stat3 inhibitor

Zebrafish Chemical Modulation of Tgfb1a

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For chemical treatments, 20 5-dpf zebrafish larvae fed with 10% cholesterol-infused diet were co-exposed with either 1 μM of JSI124, a Stat3 inhibitor (Tocris), or LY294002, a PI3K inhibitor (Tocris), for 7 days. For induction of tgfb1a expression from tgfb1a+ transgenic zebrafish, 1, 2 or 3 μM of mifeprisone (Sigma) were used to incubate 3-month-old fish for 6 weeks. For inhibition of Smad and Erk activity, 1 μM PD169316 (Sigma) or U0126 (Sigma) was added to tgfb1a+ zebrafish after 3 weeks of 3 μM mifepristone induction. All chemical dosages were selected based on the highest all-survival concentrations.

Yan C., Yang Q., Shen H.M., Spitsbergen J.M, & Gong Z. (2017). Chronically high level of tgfb1a induction causes both hepatocellular carcinoma and cholangiocarcinoma via a dominant Erk pathway in zebrafish. Oncotarget, 8(44), 77096-77109.

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Phagocytic Function of Resident Peritoneal Macrophages

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Resident peritoneal MΦ were collected from naïve WT and DRV2-KO mice and plated onto 96-well plates (0.5×10⁵ cells/well). RvD2 (1 pM-10 nM) or vehicle controls was incubated with MΦ for 15 min at 37°C, followed by incubation with FITC-labeled serum-treated zymosan particles at 10:1 ratio (zymosan: MΦ) or Baclight Green-labeled E. coli at 50:1 ratio (E. coli : MΦ) for 60 min at 37 °C. Plates were gently washed, extracellular fluorescence quenched by trypan blue, and phagocytosis determined by measuring total fluorescence (Ex 493/Em535 nm) using a fluorescent plate reader (Molecular Probes). The following inhibitors were added together with RvD2 or vehicle control: PKA inhibitor H89 (3μM; Sigma-Aldrich), pERK1/2 inhibitor (50μM; Tocris) and STAT3 inhibitor (100μM; Tocris)

Chiang N., de la Rosa X., Libreros S, & Serhan C.N. (2016). Novel Resolvin D2 receptor axis in infectious inflammation. Journal of immunology (Baltimore, Md. : 1950), 198(2), 842-851.

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