Human adipose tissue was isolated from three healthy female donors (ages 58, 46, and 43 years, respectively). Liposuction was performed in the abdominal and flank regions using a 6-mm cannula and Klein’s tumescent solution. Excess fluid was removed by decantation, and the resulting tissue was processed using the Lipogems 240 device (Lipogems International SpA, Milan, Italy) according to manufacturer instructions to generate a pure tissue fraction devoid of excess blood and debris. Briefly, adipose tissue was injected in the processing canister containing 37 °C Ringer acetate (Fresenius Kabi, Bad Homburg, Germany). The tissue was processed by repeated shaking and rinsing until the aspirate appeared light yellow, and the wash liquid was clear. Excess liquid was decanted, leaving a pure yellow lipoaspirate adipose (LAT) fraction. All procedures were performed following approval and written informed consent by the Regional Ethics Committee of Gothenburg (Dnr: 624-16). This study was conducted in accordance with national guidelines and regulations.
Ringer acetate
Manufactured by Fresenius
Sourced in Sweden
Ringer acetate is a sterile, isotonic, electrolyte solution used for fluid and electrolyte replacement in medical settings. It contains a balanced combination of sodium, potassium, calcium, and acetate ions. This solution is designed to help maintain the body's fluid and electrolyte balance.
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Lab products found in correlation
6 protocols using ringer acetate
1
Isolation of Pure Adipose Fraction
2
Ototoxic Deafening and GDNF Therapy in Guinea Pigs
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Cochleae from 24 normally hearing albino guinea pigs were transtympanically and bilaterally deafened by the ototoxic drug neomycin. Animals that did not attain a threshold shift of 60 dB SPL or higher were excluded from the study. After deafening, the animals were divided into normal (cochleae = 6), 1‐week (cochleae = 6), 4‐week (cochleae = 6), 7‐week (cochleae = 6), 10‐week (cochleae = 3), 12‐week (cochleae = 6), and 18‐week (cochleae = 6) groups. From the 12‐week group, six animals were divided into two groups. One group (n = 3) received a stimulus electrode mimicking a CI and an osmotic pump for cochlear infusion of the neurotrophic factor GDNF (Amgen, Thousand Oaks, CA) for 4 weeks (Maruyama et al., 2008 ). The other group (n = 3) served as a control group and received a CI and an osmotic pump containing artificial perilymph (AP; Ringer‐acetate; Fresenius Kabi Sweden). For animals staying in the experiment beyond 1 week, click‐evoked ABRs were measured every third week. At the end of the experiment, frequency‐specific ABRs were recorded at 4, 8, 12.5, and 20 kHz. After 4 weeks of treatment, the pumps were removed, but the animals stayed in the study for an additional 2 weeks (Fig. 1 ). To reduce the number of animals used in the study, the right ear from the implanted subjects served as the 18‐week group.
3
Isolated Limb Perfusion Protocol
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In all patients, anesthesia was induced with propofol (1.5–2.5 mg/kg), fentanyl (1.0–3 μg/kg), rocuronium (0.6 mg/kg), and maintained with sevoflurane. The ECC circuit was primed with 500 mL of Ringer-Acetate (Fresenius Kabi AB, Uppsala, Sweden), 100 mL Tribonat (Fresenius Kabi AB), 100 mL Albumin 200 g/L (Baxalta, Illinois City, USA), and 2500 IU heparin (LEO Pharma, Ballerup, Denmark) for leg perfusion. The priming solution for arm perfusion was the same as for the leg except for 1 unit of packed red cells (250 mL) which was added together with only 250 mL of Ringer-Acetate. The difference in prime regime between extremities is due to potential hemodilution anemia in arms related to large prime volume/low surface area in arms compared to legs. The ILP technique, ECC assembling, leakage monitoring, and temperature measurements were performed according to clinical routine as described by Corderfeldt et al.32 (link)
4
Infusion of NO-donor PDNO
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The NO-donor PDNO was prepared as has been previously reported [10 (link)] and infused using syringe pumps (Alaris CC, Cardinal Health Rulle, Switzerland). A crystalloid hydration solution infused with 1 mL kg−1 h−1 (Ringer-Acetate, Fresenius Kabi) served as the placebo drug.
5
Brain Injury Biomarkers in Cardiac Surgery
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The main study was a prospective, randomized, single‐center, double‐blind controlled study. The patients were randomized 1:1 to either a dextran‐based priming solution (PrimECC, XVIVO Perfusion AB, Gothenburg, Sweden) or a crystalloid‐based priming solution (Ringer acetate (Fresenius Kabi AB, Uppsala, Sweden) and mannitol (Fresenius Kabi AB)). The primary endpoint was oncotic pressure during CPB. The study’s main results have been published elsewhere.14 For the present secondary analysis, blood samples for analysis of five brain injury markers (GFAP, S100B, NSE, tau, and NfL) and the blood–brain barrier injury marker β‐trace protein were collected from the arterial line at three time points: before surgery (after induction of anesthesia); 2 h after CPB, and 24 h after CPB. At the same time points, the degree of hemolysis was measured as it is known that hemolysis interacts with the analysis of NSE.15 Patient data, type of surgery, CPB time, aortic cross‐clamp time and total operating time were registered for all patients. Concentrations of brain injury markers 2 h postoperatively were correlated to patient age, operation time, and hemolysis. No neurocognitive tests were performed.
6
Intravenous Glucose Tolerance Test in Cats
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The IVGTT followed a previously described protocol[16 (link)]. Following at least 3 h of cage rest, a glucose bolus (1 g/kg body weight) was injected through the cephalic catheter over 30 seconds. Through the other catheter, 2 mL samples of blood were collected into ice-chilled 2-mL EDTA tubes at times 0 (prior to injection), 2, 5, 10, 15, 30, 45, 60, 90 and 120 min after the glucose injection. Prior to each blood sampling, 0.5 mL of blood was drawn into a separate syringe to avoid collection of diluted catheter blood; this was re-injected immediately after collection of the actual sample. Catheter patency was maintained by flushing with 0.5 mL of heparinized saline. Blood samples were kept on ice after collection, centrifuged within 1 h at 3500 rpm for 10 min at 4°C and stored at -80°C for later analysis. After completion of the IVGTT, cats were hospitalized overnight, offered a meal and placed on intravenous fluids (Ringer Acetate, Fresenius Kabi, Uppsala, Sweden) for 8 h to replenish circulatory volume. Food was withheld after 10 PM.
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