3 mL samples were placed in BD vacutainer tubes containing EDTA for trace element determination for measuring Pb and Cd (K2 EDTA tube, Vacutainers®; BectonDickinson, Franklin Lakes, NJ, USA). Graphite furnace atomic absorption spectrometry using Zeeman background correction was used for measuring Pb and Cd (Perkin Elmer AAnalyst 600; Perkin Elmer, Turku, Finland). The Neodin Medical Institute, a laboratory certified by the Korea Ministry of Health and Welfare conducted all blood metals analysis. Commercial references were used for internal quality assurance and control (Lyphochek® Whole Blood Metals Control; Bio-Rad, Hercules, CA, USA); the coefficients of variation of lead was 2.65–6.50% and cadmium was 0.95–4.82%. This institute passed the German External Quality Assessment Scheme (operated by Friedrich-Alexander University) and the Quality Assurance Program (operated by the Korea Occupational Safety and Health Agency) for external quality assurance and control. The Ministry of Labor which is one of the designated laboratories for analysis of specific chemicals such as heavy metals and certain organic chemicals certified the institute. The method detection limits of lead was 0.207 µg/dL and cadmium was 0.081 µg/L. All samples exceeded these detection limits.
Lyphochek whole blood metals control
Manufactured by Bio-Rad
Sourced in United States
Lyphochek® Whole Blood Metals Control is a laboratory quality control material used to monitor the performance of instruments and methods that measure the concentration of metals in whole blood samples. It provides a matrix-matched material for quality assurance purposes.
Automatically generated - may contain errors
7 protocols using lyphochek whole blood metals control
1
Trace Element Analysis of Blood
2
Measuring Blood Mercury Levels
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To measure the levels of mercury in whole blood, 3-mL blood samples were obtained from the participants after an 8-h overnight fast. The blood mercury levels were measured using the gold amalgam collection method and the Direct Mercury Analyzer 80 (DMA-80; Milestone, Bergamo, Italy) at the Neodin Medical Institute (Seoul, South Korea), a central laboratory certified by the Korean Ministry for Health and Welfare [23 (link)]. For internal quality assurance and control, commercial reference material was used (Lyphochek® Whole Blood Metals Control; Bio-Rad, Hercules, CA, USA) with coefficients of variation of 1.59–4.86% among four reference samples. For external quality assurance and control, the Neodin Medical Institute was approved by the German External Quality Assessment at Friedrich-Alexander University and by the Quality Assurance Program at the Korea Occupational Safety and Health Agency. The limit of detection for blood mercury using this method was 0.158 μg/L, and no participants presented a blood mercury concentration below this level in this study. Blood mercury levels were categorized into quartiles (Qs) and stratified by sex and age. Q4 of blood mercury levels was defined as the highest value.
3
Measurement of Blood Lead and Cadmium
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To measure PbB and CdB levels, 3 mL samples were drawn into BD vacutainer tubes containing EDTA for trace-element determination (K2 EDTA tube, Vacutainers®; Becton Dickinson, Franklin Lakes, NJ, USA). PbB and CdB were measured by graphite furnace atomic absorption spectrometry with Zeeman background correction (Perkin Elmer AAnalyst 600; Perkin Elmer, Turku, Finland).
Analysis of all blood metals was performed by the Neodin Medical Institute, a laboratory certified by the Korea Ministry of Health and Welfare. For internal quality assurance and control, commercial reference materials were used (Lyphochek® Whole Blood Metals Control; Bio-Rad, Hercules, CA, USA); the coefficients of variation were 2.65% to 6.50% (lead) and 0.95% to 4.82% (cadmium). As part of external quality assurance and control, this institute passed the German External Quality Assessment Scheme (operated by Friedrich-Alexander University) and the Quality Assurance Program (operated by the Korea Occupational Safety and Health Agency). The institute was also certified by the Ministry of Labor as one of the designated laboratories for analysis of specific chemicals, including heavy metals and certain organic chemicals. The method detection limits were 0.207 µg/dL for lead and 0.081 µg/L for cadmium. All samples were above these detection limits.
Analysis of all blood metals was performed by the Neodin Medical Institute, a laboratory certified by the Korea Ministry of Health and Welfare. For internal quality assurance and control, commercial reference materials were used (Lyphochek® Whole Blood Metals Control; Bio-Rad, Hercules, CA, USA); the coefficients of variation were 2.65% to 6.50% (lead) and 0.95% to 4.82% (cadmium). As part of external quality assurance and control, this institute passed the German External Quality Assessment Scheme (operated by Friedrich-Alexander University) and the Quality Assurance Program (operated by the Korea Occupational Safety and Health Agency). The institute was also certified by the Ministry of Labor as one of the designated laboratories for analysis of specific chemicals, including heavy metals and certain organic chemicals. The method detection limits were 0.207 µg/dL for lead and 0.081 µg/L for cadmium. All samples were above these detection limits.
4
Determination of Blood Cadmium Levels
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To determine the concentrations of BCd (μg/L) in venous whole blood, blood samples (3 mL) were collected from each participant into blood collection tubes containing ethylenediaminetetraacetic acid (EDTA) for determination of trace elements (BD Vacutainer K2-EDTA tubes; Becton Dickinson, Franklin Lakes, NJ, USA). BCd was measured by graphite furnace atomic absorption spectrometry using the model AAnalyst 600 (Perkin Elmer, Turku, Finland).
Analyses of all blood metals were conducted by the Neodin Medical Institute, a laboratory certified by the Korea Ministry of Health and Welfare. Internal quality assurance and control were established using commercial reference materials (Lyphochek Whole Blood Metals Control; Bio-Rad Laboratories, Hercules, CA, USA). For external quality assurance and control, the Neodin Medical Institute fulfilled the requirements of both the German External Quality Assessment Scheme (operated by Friedrich-Alexander University) and the Quality Assurance Program (operated by the Korea Occupational Safety and Health Agency). The institute was also certified by the Ministry of Labor as a designated laboratory for the analysis of specific chemicals, including heavy metals. The method detection limit for Cd was 0.016 μg/L. All samples were above this detection limit.
Analyses of all blood metals were conducted by the Neodin Medical Institute, a laboratory certified by the Korea Ministry of Health and Welfare. Internal quality assurance and control were established using commercial reference materials (Lyphochek Whole Blood Metals Control; Bio-Rad Laboratories, Hercules, CA, USA). For external quality assurance and control, the Neodin Medical Institute fulfilled the requirements of both the German External Quality Assessment Scheme (operated by Friedrich-Alexander University) and the Quality Assurance Program (operated by the Korea Occupational Safety and Health Agency). The institute was also certified by the Ministry of Labor as a designated laboratory for the analysis of specific chemicals, including heavy metals. The method detection limit for Cd was 0.016 μg/L. All samples were above this detection limit.
5
Assessing Heavy Metals in Blood
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To assess the levels of heavy metals in whole blood, 1-mL blood samples were drawn into standard commercial evacuated tubes containing sodium heparin (Vacutainer®, BD, Franklin Lakes, NJ, USA). Blood cadmium and lead were measured by graphite furnace atomic absorption spectrometry with Zeeman background correction (Perkin Elmer AAS800, Perkin Elmer, Waltham, MA, USA). All blood metal analysis was carried out by Neodin Medical Institute, a laboratory certified by the Korean Ministry of Health and Welfare.
For the internal quality assurance and control program, commercial reference materials were used (Lyphochek® Whole Blood Metals Control, Bio-Rad, Hercules, CA, USA). The method detection limits for blood cadmium and lead in the present study were 0.056 μg/L and 5.8 μg/L, respectively. For participants with blood levels below the detection limit, we assigned a level equal to the detection limit divided by the square root of 2 (23 (link)).
For the internal quality assurance and control program, commercial reference materials were used (Lyphochek® Whole Blood Metals Control, Bio-Rad, Hercules, CA, USA). The method detection limits for blood cadmium and lead in the present study were 0.056 μg/L and 5.8 μg/L, respectively. For participants with blood levels below the detection limit, we assigned a level equal to the detection limit divided by the square root of 2 (23 (link)).
6
Blood Manganese Analysis Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Blood Mn was measured using whole blood at the Neodin Medical Institute (certified by the Korean Ministry of Health and Welfare) in Seoul, Korea, following a standardized protocol. Blood Mn was analyzed by graphite furnace atomic absorption spectrometry with Zeeman background correction (Perkin Elmer AAS800, Perkin Elmer, Turku, Finland). The limit of detection was 0.016 μg/dL for blood Mn. For internal quality assurance and control, standard reference materials were obtained from Bio-Rad (Lyphochek® Whole Blood Metals Control). The inter-assay coefficients of variation ranged from 0.95% to 4.82% for blood Mn samples (reference values were 0.98, 1.18, 2.46, and 3.28 μg/dL). During the survey, overnight fasting venous blood samples were collected. The collected blood samples were properly processed, refrigerated, and transported in cold storage to the Neodin Medical Institute in Seoul, Korea.
7
Biomonitoring of Toxic Metals in Blood
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Blood samples were drawn into trace metal-free tubes, and analyzed by a laboratory procedure certified by the Korean Ministry of Health and Welfare. Blood lead (Pb) and cadmium (Cd) levels were determined by atomic absorption spectrometry (SpectrAA-800 Zeeman, Varian, Australia). Blood aluminum (Al) was analyzed by inductively coupled plasma optical emission spectrometry (SPECTRO Midex, SPECTRO Analytical Instruments GmbH, Germany). Commercial reference materials were used for internal quality assurance and control (Lyphochek® Whole Blood Metals Control; Bio-Rad, Hercules, CA, USA). The coefficients of variation for blood metals were ≤ 10%. External quality control was provided by the Korean Occupational Safety & Health Agency (KOSHA), and the German External Quality Assessment Scheme (G-EQUAS). The limits of detection (ng/ml) in blood for this procedure were: Pb (0.02); Cd (0.01); and Al (0.01). There were no values below limits-of-detection (LOD) levels for the three metals of interest.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!