Freshly isolated dCD4+ T cells co-cultured with Tim-3+dMφs or Tim-3−dMφs at a 1: 1 ratio. In some experiments, Tim-3+dMφs were pretreated with anti-Tim-3 (10 μg/ml, clone F38-2E2, BioLegend, U.S.A.), or anti-CD132 (10 μg/ml, clone TUGh4, BioLegend, U.S.A.), or anti-IL-4 (10 μg/ml, clone F38-2E2, BioLegend, U.S.A.). Phorbol 12-myrstate 13-acetate (PMA) (50 ng/ml, Biolegend, U.S.A.), ionomycin (1 μg/ml, Biolegend, U.S.A.) and brefeldin A (10 mg/ml, BioLegend, U.S.A.), were added 4 h before the end of the 48 h co-culture. The supernatants were then collected for intracellular cytokine analysis of T cells.
Clone f38 2e2
Clone F38-2E2 is a monoclonal antibody that recognizes the human CD40 antigen. CD40 is a cell surface receptor that belongs to the tumor necrosis factor receptor superfamily and is expressed on B cells, dendritic cells, macrophages, and other cell types. The antibody can be used for flow cytometry and other immunoassays to detect and analyze CD40-expressing cells.
Lab products found in correlation
5 protocols using clone f38 2e2
Trophoblast-Decidual Cell Interactions
Freshly isolated dCD4+ T cells co-cultured with Tim-3+dMφs or Tim-3−dMφs at a 1: 1 ratio. In some experiments, Tim-3+dMφs were pretreated with anti-Tim-3 (10 μg/ml, clone F38-2E2, BioLegend, U.S.A.), or anti-CD132 (10 μg/ml, clone TUGh4, BioLegend, U.S.A.), or anti-IL-4 (10 μg/ml, clone F38-2E2, BioLegend, U.S.A.). Phorbol 12-myrstate 13-acetate (PMA) (50 ng/ml, Biolegend, U.S.A.), ionomycin (1 μg/ml, Biolegend, U.S.A.) and brefeldin A (10 mg/ml, BioLegend, U.S.A.), were added 4 h before the end of the 48 h co-culture. The supernatants were then collected for intracellular cytokine analysis of T cells.
TIGIT and TIM-3 Blockade Enhances CD8+ T-cell Function
Modulating CD8+ T cell function
Decidual CD8+ T Cell Immune Modulation
Cytokine Production in CD4+ T Cells
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