C57Bl/6 mice pups (37 of each sex) were obtained from Harlan Laboratories S.R.L. (S.Piero al Natisone, Italy). Immediately after weaning, animals were randomly group-housed in cages in a temperature-controlled environment (22±2°C) and on a 12 hours light/dark schedule, and fed ad-libitum with control diet (CTRL, D12328, Research Diets, New Brunswick, NJ) or HFHC diet (HFHC: D12331, Research Diets, New Brunswick, NJ), plus 42g/L fructose/sucrose in drinking water, for a total of 16 weeks [25 (link)]. Four experimental checkpoints were established (4, 8, 12 and 16 weeks of diet), in which part of the animals was sacrificed. Animal care and procedures were conducted in accordance with the Italian Law (decree 116–92) and by European Community directive 86-609-EEC. All experimental protocol were approved by the local committee of the Medical Research Institute and by the National Authority (Ministero della Salute—Direzione generale della sanità animale e dei farmaci veterinari—Ufficio VI, Rome, Italy, under the Article 7, D.lgs 116/92). The maximal effort was done to reduce the number of animals used and their sufferance in the respect of the 3R rule.
D12328
Manufactured by Research Diets
Sourced in United States
The D12328 is a specialized laboratory equipment designed for research purposes. It functions as a precision instrument for controlled environmental conditions, enabling researchers to conduct various experiments and studies. The core function of this product is to maintain and regulate specific environmental parameters, such as temperature, humidity, or other relevant factors, as required by the research protocol. No further interpretation or extrapolation on the intended use of this product is provided.
Automatically generated - may contain errors
Lab products found in correlation
D12331, by Research Diets (4 mentions)
D12492, by Research Diets (2 mentions)
D12330, by Research Diets (2 mentions)
D12451, by Research Diets (2 mentions)
Elisa kit, by Abcam (2 mentions)
Echomri 100, by Echo Medical Systems (1 mentions)
Minispec body composition analyzer, by Bruker (1 mentions)
C57bl 6j mice, by Janvier Labs (1 mentions)
9 protocols using d12328
1
High-fat, high-carb diet in mice
2
Characterization of Pde9a-deficient Mice
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Animal studies were conducted at Sanford Burnham Prebys Medical Discovery Institute (SBP), Vanderbilt University Medical Center (VUMC), and Johns Hopkins University and School of Medicine (JHSOM). Pde9a−/− mice on a C57BL/6J background were previously described (37 (link)). Mice were maintained on a 12-h light/dark cycle with two to five animals per cage and ad libitum food and water. At 6 weeks of age, SBP and VUMC mice received nutrient-matched Surwit diets: a control low-fat diet (LFD) (10.5% calories from fat: D12328; Research Diets) or high-fat diet (HFD) (58.0% calories from fat: D12330; Research Diets). JHSOM mice were fed HFD (60.0% calories from fat: D12492; Research Diets). Mice were weighed twice weekly, and body composition was measured using a Minispec Body Composition Analyzer (Bruker) at SBP and Vanderbilt Mouse Metabolic Phenotyping Center (VMMPC) or EchoMRI-100 (Echo Medical Systems) at JHSOM. Mice were fasted for 5-h prior to euthanasia by CO2 asphyxiation and exsanguination via cardiac puncture. All procedures were approved by the Institutional Animal Care and Use Committees at SBP, VUMC, and JHSOM.
3
High-fat diet effects on gestating mice
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Female breeders were assigned to the control diet (CD, n = 47) or the high-fat diet (HFD, n = 48) groups. Diets were administered ad libitum for 10 weeks before mating and throughout gestation (for a total of 13 weeks) until G16, when both HFD and CD were replaced with SD to prevent cannibalistic behaviors and pups’ mortality [25 (link)]. HFD (D12331, energy 5.56 kcal/g, fat 58%, carbohydrate 25.5% and protein 16.4) and CD (D12328, energy 4.07 kcal/g, fat 10.5%, carbohydrate 73.1%, and protein 16.4%) were provided by Research Diets Inc., USA.
4
Transgenic Mice Model of Obesity
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Adult male transgenic mice overexpressing H6PDH under the adipocyte fatty acid-binding protein (aP2) gene (aP2/H6PDH Tg) and their age-matched (10 weeks) wild-type (WT) C57blk/6 control mice were bred and genotyped, as previously described.19 (link) Eight animals have been used for each experimental group, the numbers of animal used were determined to achieve desired power of 0.8. Animals were housed in standard SPF conditions under a 12:12-h light-dark cycle with water ad libitum. Mice were maintained on either a control diet (Research Diets D12328) or a HFD (45 % energy from Fat, Research Diets D12451; Research Diets Inc., New Brunswick, NJ, USA) for 4 or 14 weeks. For each genotype, mice are divided randomly into the control diet or HFD group. Food intake and body weights were recorded weekly. At the end of the experiments, blood samples were collected, and adipose fat depots were weighed, dissected and frozen rapidly in liquid nitrogen. Animal protocols were approved by the Institutional Animal Care and Use Committee of the Charles R. Drew University (Los Angeles, California, USA). Blood samples were analyzed for insulin and FFA levels using ELISA kit (Abcam, Cambridge, MA, USA), as previously described.18 (link)
5
Transgenic Mice Model of Obesity
6
GPR120 Agonist Modulates Diet-Induced Obesity
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Mini-pumps delivered either vehicle or GPR120 agonist (1 µM/d; ~42nM/h) at a flow rate of 0.25 µL/h into the lateral ventricle. Food intake and body weight measures began 3 days following cannula and mini-pump implantation. Mice initially fed a chow diet were separated into 3 weight-matched groups (n=5–8/group): (1) vehicle infusion on an ingredient-matched, low-fat diet (LFD; 4.07 kcal/gm: 10.5% kcal fat, 16.4% kcal protein, 73.1% kcal carbohydrates, D12328, Research Diets, Inc., New Brunswick, NJ); (2) vehicle infusion on a HFD (5.56 kcal/gm: 58% kcal fat, 16.4% kcal protein, 25.5% kcal carbohydrates, D12331, Research Diets, Inc.); and (3) GPR120 agonist infusion on a HFD. Mice received their respective diet for 3 weeks.
7
High-Fat and Low-Fat Diet Effects
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Experimental diets consisted of a high-fat diet (HFD) and a low-fat diet (LFD), as previously described[2 (link),9 (link)]. These diets differed in amounts of fats from hydrogenated coconut oil. The HFD contained 58.0% kcal/g of fat, 25.5% kcal/g of carbohydrate, and 16.4% kcal/g of protein (D12330; Research Diets; New Brunswick, NJ). The LFD contained 10.5% kcal/g of fat, 73.1% kcal/g of carbohydrate, and 16.4% kcal/g of protein (D12328; Research Diets; New Brunswick, NJ). Diets were otherwise matched for all micronutrients. At 30 days of age, male WT andApcMin/+ mice were assigned to either the HFD or LFD, resulting in four groups according to genotype and diet: WT-LFD, WT HFD,ApcMin/+-LFD,ApcMin/+-HFD. Mice were fed experimental diets ad libitum for three days (30–33 days of age). Biological replicate mice were used for each combination of genotype and diet type. All animal procedures were evaluated and approved by the Institutional Animal Care and Use Committee of CWRU School of Medicine, Protocol Number 2020-016.
8
High-Fat Diet Induced Obesity Study
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Male and female C57Bl/6 mice (6–8 weeks of age) were divided into four groups and fed either a normal diet (ND; Research Diets, D12328, Carbohydrate 73%, Fat 11%, and Protein 16% kcal) or a high-fat diet (HFD; Research Diets, D12492, 60% of fat calories; 20% of protein and 20% of carbohydrate) ad libitum. Tap water was provided ad libitum. Mice were monitored for 14 weeks. Body weight was measured weekly. At the end of the experiments, mice were euthanized by carbon dioxide (CO2) asphyxiation, then gonadal, retroperitoneal, visceral, subcutaneous, subscapular brown adipose tissue, heart, liver, and kidneys were isolated and weighed for adiposity and cardio-renal characterization.
9
Maternal Diet-Induced Obesity Model
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To generate the diet-induced maternal obesity model, 4-week-old female C57Bl/6 J mice (Janvier Labs, Le Genest-Saint-Isle, France) were fed an HFHS diet (Research Diets, D12331) comprising 58% fat, 26% carbohydrates [17% sucrose], and 16% proteins or a control diet (Research Diets, D12328) for 6 weeks prior mating13 (link). Eight- to 12-week-old male mice fed on chow diet were used for mating and fed an in-house chow diet (R34, Lantmännen, Kimstad, Sweden). First-generation female offspring were subjected to phenotypic testing which has been described in detail elsewhere13 (link).
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