Melting points were measured with a Yanaco micro melting point apparatus MP model. IR spectra were recorded on a PerkinElmer Spectrum One FT-IR spectrometer using ATR method. 1H and 13C NMR spectra were recorded on a Varian-400 (400 MHz) or a Varian-500 (500 MHz) FT NMR spectrometer. High-resolution mass spectral data by ESI and GC-FI were acquired on a Thermo Fisher Scientific LTQ Orbitrap XL and JEOL JMS-T100 GCV 4G, respectively. Photoabsorption spectra were observed with a SHIMADZU UV-3150 spectrophotometer. Fluorescence spectra were measured with a Hitachi F-4500 spectrophotometer. The fluorescence quantum yields were determined by a HORIBA FluoroMax-4 spectrofluorometer by using a calibrated integrating sphere system. The addition of water to acetonitrile solutions containing DJ-1 was made by weight percent (wt%). The determination of water in acetonitrile was done with a MKC-610 and MKA-610 Karl Fischer moisture titrator (Kyoto Electronics manufacturing Co., Ltd.) based on Karl Fischer coulometric titration for below 1.0 wt% and volumetric titration for 1.0–40 wt%, respectively.
F 4500 spectrophotometer
Manufactured by Hitachi
Sourced in Japan
The Hitachi F-4500 spectrophotometer is a high-performance instrument designed for accurate and reliable spectroscopic analysis. It features a wide wavelength range, high-resolution optics, and advanced detection capabilities to provide precise measurements of absorption, transmission, or emission spectra. The F-4500 is a versatile tool suitable for a variety of applications in scientific research and industrial settings.
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Lab products found in correlation
Uv 3150 spectrophotometer, by Shimadzu (2 mentions)
Su8010, by Hitachi (2 mentions)
Uv 2550, by Shimadzu (2 mentions)
S 4800, by Hitachi (2 mentions)
Uv 1700, by Shimadzu (2 mentions)
Tecnai g2, by Philips (2 mentions)
Fluoromax 4 spectrofluorometer, by Horiba (1 mentions)
Spectrum one ftir spectrometer, by PerkinElmer (1 mentions)
Jms t100 gcv 4g, by JEOL (1 mentions)
D8 advance, by Bruker (1 mentions)
Jem 2100, by Shimadzu (1 mentions)
Lamda 25 spectrophotometer, by PerkinElmer (1 mentions)
Asap 2020 system, by Micrometrics (1 mentions)
Cary 50 uv visible spectrophotometer, by Agilent Technologies (1 mentions)
Jem 2100f transmission electron microscope, by JEOL (1 mentions)
K alpha x ray photoelectron spectrometer, by Thermo Fisher Scientific (1 mentions)
Evolution 300, by Thermo Fisher Scientific (1 mentions)
Ls 110, by Konica Minolta (1 mentions)
Epl 375 picosecond pulsed diode laser, by Edinburgh Instruments (1 mentions)
Fls980 spectrometer, by Edinburgh Instruments (1 mentions)
22 protocols using f 4500 spectrophotometer
1
Physicochemical characterization of organic compounds
2
Characterization of Nanostructured Materials
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The SEM images and EDS analysis were acquired using a Hitachi SU-8010 equipped with an EDX analyzer operated at an accelerating voltage of 5 kV. The TEM images were obtained on a JEM 2100 operating at 200 kV. The UV-vis diffuse reflectance spectroscopy (DRS) measurements were obtained on a UV-vis spectrometer (Shimadzu UV-2550) using BaSO4 as a reference standard. The specific surface area of the samples was measured by the Brunauer–Emmett–Teller (BET) method using nitrogen adsorption and desorption isotherms on a Micrometrics ASAP 2020 system. The UV-vis spectra were obtained on a Perkin Elmer Lamda 25 spectrophotometer. The NMR spectra were recorded on a Mercury Vx-300 MHz NMR spectrometer. XRD patterns were obtained on a Bruker D8-Advance. The luminescence spectra were measured using a Hitachi F-4500 spectrophotometer in MeOH at room temperature. A 500 W xenon lamp (CHFXQ 500 W, Global xenon lamp power) with a λ ≥ 420 nm optical filter, AM 1.5 optical filter and a heat cut-off filter provided visible light or simulated sunlight illumination.
3
Optical Measurements for pH Sensing
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The experimental
setup for the optical measurement is sketched
inFigure 3 A,B. It
consists mainly of a light source (430 nm LED or 532 nm green laser),
an objective lens to focus the light, and a charge-coupled device
(CCD) spectrometer to collect the PL signal in an integration time
of 500 ms. The sensor tip was placed in a Petri dish as shown inFigure 3 C,D in order to test
the pH value of buffer solutions inside. The light was sent from the
LED or laser connected with the optical fiber via an adaptor. The
fluorescence from the fiber was read with the CCD spectrometer having
measurements in the wide spectrum range of 200–1100 nm to record
the emission spectrum. To prevent undesirable photoexcitation from
room lights, the measurement was performed in the dark.
Besides the optical setup
on the sensor, spectroscopic analysis
on the sensing molecules is also made. Absorbance measurements for
the sensing film and solutions were conducted on a Cary 50 UV–Visible
Spectrophotometer with the sensing film coated on a glass slide and
the test solution placed in a 1.0 cm path length glass cuvette. A
Hitachi F-4500 Spectrophotometer was used to record the fluorescence
emission spectra. pH value measurements were performed on the waterproof
ExStik PH100 pH meter (Extech) calibrated with standard pH buffers
of 4, 7, and 10. All measurements were carried out at room temperature.
setup for the optical measurement is sketched
in
consists mainly of a light source (430 nm LED or 532 nm green laser),
an objective lens to focus the light, and a charge-coupled device
(CCD) spectrometer to collect the PL signal in an integration time
of 500 ms. The sensor tip was placed in a Petri dish as shown in
the pH value of buffer solutions inside. The light was sent from the
LED or laser connected with the optical fiber via an adaptor. The
fluorescence from the fiber was read with the CCD spectrometer having
measurements in the wide spectrum range of 200–1100 nm to record
the emission spectrum. To prevent undesirable photoexcitation from
room lights, the measurement was performed in the dark.
Besides the optical setup
on the sensor, spectroscopic analysis
on the sensing molecules is also made. Absorbance measurements for
the sensing film and solutions were conducted on a Cary 50 UV–Visible
Spectrophotometer with the sensing film coated on a glass slide and
the test solution placed in a 1.0 cm path length glass cuvette. A
Hitachi F-4500 Spectrophotometer was used to record the fluorescence
emission spectra. pH value measurements were performed on the waterproof
ExStik PH100 pH meter (Extech) calibrated with standard pH buffers
of 4, 7, and 10. All measurements were carried out at room temperature.
4
Synthesis and Characterization of Boron Nitride Quantum Dots
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BN-CDs were prepared by microwave hydrothermal method as described by Ma et al.[49]with minor modifications. Citric acid (5 g), ethylenediamine (2 mL) and boric acid (2 g) were dissolved in 30 mL deionized water. The mixed solution was transferred to an autoclave, and the reactor was connected to a microwave-assisted synthesizer (XH-300A +, Beijing Xianhu Technology Development Co., Ltd., Beijing, China) to react at 200 ℃ for 1 h. Then the solution was cooled down to room temperature, and dialyzed with MWCO of 3500 Da for 24 h. The dialysate was concentrated by vacuum rotary evaporator, and the concentrated solution was lyophilized. Fluorescence spectra were obtained by an F-4500 spectrophotometer (Hitachi Ltd., Japan). Absorption spectra were recorded on a UV − vis spectrophotometer (UV-2550, Shimadzu Ltd., Japan). TEM measurements were performed on a model JEM-2100F transmission electron microscope (JEOL, Japan) for characterization of the shape and size of BN-CDs. XPS spectra were used to characterize the chemical composition using a K-Alpha X-ray Photoelectron Spectrometer (Thermo Fisher Scientific, USA).
5
Spectroscopic Characterization of Caged Compounds
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All absorption and fluorescence emission measurements were performed in standard quartz cuvettes (1.00 cm optical pathlength, Hellma‐Analytics) with various maximum volumes. UV/Vis absorption was recorded using a commercially available Evolution 300 (ThermoScientific) or our custom‐made set up equipped with an Ocean Optics DH‐mini light source and USB4000 detector, a thermostatic cuvette holder (Thorlabs), all controlled by our in‐house programmed PHITS (Photoswitch Irradiator Test Suite) software, which was written in LabVIEW. For more details see Reinfelds et al.[29] This setup and software were also used for our chemical actinometry. Reference compound was an indolylfulgide photoswitch. A concentrated solution of the fulgide (500–1000 μm ) was irradiated with the respective light source (Thorlabs mounted LED, λmax=420 nm or 530 nm) to convert the photoswitch from its 1Z form to 1C or the other way round. Afterward, the caged glutamic acid of interest could be irradiated with known photon flux. Steady‐state fluorescence emission was recorded using a Hitachi F‐4500 spectrophotometer. The optical density (OD) was set lower than or equal to 0.1 for fluorescence spectra, otherwise checked for consistency. Details of the set ups for two‐photon induced spectroscopy have been described previously.[22 , 35 , 36 ] See the Supporting Information for additional data.
6
Fluorescence Spectroscopy of ThT-RNA Interactions
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The fluorescence spectra were acquired with a Hitachi F-4500 spectrophotometer at 25±1 °C, which was equipped with a temperature-controlled circulator. A quartz cuvette with a 10-mm path length was used in all of the experiments. In the fluorescence measurements, both the excitation and emission slits were 5 nm, and the scan speed was 240 nm/min. ThT was titrated with RNA G-quadruplex to measure the binding constants, and the fluorescence intensity at 440 nm was plotted as a function of the RNA concentration. The data were fitted according to a 1:1 binding model. The titration experiments were performed by increasing the RNA G-quadruplex concentrations from 0.125 to 8 μM ThT, which was added to the different RNA concentration solutions with gentle stirring for 10 min, and then, the samples were maintained in darkness for 2 hours prior to the measurements.
The fluorescence lifetime measurements were recorded on a time-correlated single photon counting FLSP920 system. From the measured decay traces, the data were fitted with a multi-exponential decay, and χ2 was less than 1.1.
The fluorescence lifetime measurements were recorded on a time-correlated single photon counting FLSP920 system. From the measured decay traces, the data were fitted with a multi-exponential decay, and χ2 was less than 1.1.
7
Fluorescence Spectra Characterization
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Samples were diluted in 10 mM Tris-HCl, 10 mM EDTA, pH 8.0 buffers and fluorescence spectra were recorded on a Hitachi F-4500 spectrophotometer at room temperature. Excitation spectra were measured between 350 and 500 nm with the emission wavelength fixed at 508 nm. Emission spectra were measured between 450 and 600 nm with the excitation wavelength fixed at 396 nm.
8
Fluorescence Spectroscopy of RNA G4-CyT Binding
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Fluorescence spectra were acquired at 25 ± 1 °C, using a Hitachi F-4500 spectrophotometer equipped with a temperature-controlled circulator. A 10-mm path length quartz cuvette was used in all experiments. For fluorescence measurements, both excitation and emission slits were 5 nm, and the scan speed was set at 240 nm/min. CyT was titrated with RNA G4 for measurement of the binding constants, with the fluorescence intensity at 570 nm plotted as a function of the RNA concentration. The data were fitted according to a 1:1 binding model. The titration experiments were performed by increasing RNA G4 concentrations from 0.125 to 4 μM. CyT at the specified concentration was added to solutions of different RNA concentrations, with gentle stirring for 10 min, and then the samples were kept in darkness for 2 h before measurements were taken. Fluorescence quantum yield values were acquired by the method described previously (48 ).
9
Optoelectronic Characterization of Thin Films
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The characteristics of current–voltage–luminance a were measured by a programmable Keithley model 2400 power supply and a Minolta Luminance Meter LS-110, respectively, in atmosphere conditions without any encapsulation for the devices. The spectra of the devices were obtained through Ocean Optics Maya 2000-PRO spectrometer.
The room temperature absorption/transmittance spectra were measured with an ultraviolet/visible spectrometer (UV 1700, Shimadzu) and the PL spectrum of the QDs in toluene was collected by a Hitachi F-4500 spectrophotometer under an excitation wavelength of 400 nm. The transmission electron microscopy (TEM) images were recorded on a Philips TECNAI G2 and the morphology of ZnO and AgNW films were characterized by scanning electron microscope (SEM) (Hitachi S4800). The sheet resistance (Rs) of AgNW film fabricated on a 2.5 cm × 2.5 cm glass/ZnO nanoparticle substrates was measured through four-point probe.
The room temperature absorption/transmittance spectra were measured with an ultraviolet/visible spectrometer (UV 1700, Shimadzu) and the PL spectrum of the QDs in toluene was collected by a Hitachi F-4500 spectrophotometer under an excitation wavelength of 400 nm. The transmission electron microscopy (TEM) images were recorded on a Philips TECNAI G2 and the morphology of ZnO and AgNW films were characterized by scanning electron microscope (SEM) (Hitachi S4800). The sheet resistance (Rs) of AgNW film fabricated on a 2.5 cm × 2.5 cm glass/ZnO nanoparticle substrates was measured through four-point probe.
10
Fluorescence Spectroscopy Protocol for PDMPO
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The measurements were performed with a Hitachi model F-4500 spectrophotometer. The samples were diluted to avoid inner filter effect. At each excitation wavelength, the fluorescence intensity at the emission wavelengths was measured to obtain 3D excitation–emission fluorescence matrices (EEFM). The excitation window was set to 5 nm and the emission window to 10 nm. The excitation wavelengths were scanned from 200 nm to 500 nm, and the emission wavelengths from 250 nm to 600 nm, with a 5 nm step for both emission and excitation at a scanning speed of 2400 nm min−1. The photomultiplier was set to 700 V. The obtained spectra were processed to remove the Rayleigh scattering bands using the Matlab software.
For each system and at each pH we performed between 5 and 8 measurement series, each series beginning with the PDMPO only as a reference. The maximum intensity at λex = 330 nm of the 460 nm and the 510 nm peaks was normalized to the signal of the PDMPO only. We then calculated the average and the standard deviation for each condition, an example is given onFig. 2 .
For each system and at each pH we performed between 5 and 8 measurement series, each series beginning with the PDMPO only as a reference. The maximum intensity at λex = 330 nm of the 460 nm and the 510 nm peaks was normalized to the signal of the PDMPO only. We then calculated the average and the standard deviation for each condition, an example is given on
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