Bronchoalveolar lavage fluid (BALF) was obtained by lavaging the lungs twice with 0.5 ml sterile DPBS. A Thermo Scientific Cytospin 4 Cytocentrifuge (Thermo Fisher Scientific, Waltham, MA) was immediately used to spin cells from 100 μl of BALF onto glass slides. These cells were then fixed and stained with the Diff-Quik Stain Set (Dade Behring, Inc., Newark, DE). The remaining BALF was stored at −80° C. After BALF collection, the middle and caudal lobes of the right lung, as well as the heart, spleen, and a section of the liver, were placed in RNAlater (Ambion, Austin, TX), according to the manufacturer’s instructions and stored at −80° C. The cranial lobe of the right lung was flash frozen in liquid nitrogen and stored at −80° C. The left lung was infused with 10% neutral buffered formalin, fixed for 24 hours, dehydrated in 70% ethanol, and embedded in paraffin. Whole blood was collected from the jugular veins, allowed to coagulate for 15 minutes in Serum Separator Tubes (BD Microtainer, Franklin Lakes, NJ), then centrifuged to obtain serum. Serum was stored at −80° C.
Thermo scientific cytospin 4 cytocentrifuge
Manufactured by Thermo Fisher Scientific
Sourced in Australia
The Thermo Scientific Cytospin 4 Cytocentrifuge is a laboratory instrument used to prepare cytology samples for microscopic examination. It spins samples at controlled speeds to deposit cells onto a microscope slide, allowing for the preparation of high-quality, evenly distributed specimens.
Automatically generated - may contain errors
3 protocols using thermo scientific cytospin 4 cytocentrifuge
1
Lung and Serum Sample Collection
2
Spiral Microfluidic Chip for PCa Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The efficiency of the spiral microfluidic chip for capturing PCa tumour cells was evaluated by spiking a predetermined number of DU-145 cells (ATCC HTB-81) (approximately 1000) into 50 mL of Dulbecco’s phosphate-buffered saline (DPBS). The solution was injected into the chip using a peristaltic pump (Baoding Shenchen Precision Pump Co., Ltd, Baoding, China) at a 1.7-mL/min flow rate. When the volume of DPBS in the sample tube decreased to 1 mL, enriched cells were deposited onto an adhesive glass slide (ThermoFisher Scientific, Scoresby, VIC, Australia) using a Thermo Scientific™ Cytospin™ 4 Cytocentrifuge (ThermoFisher Scientific, Scoresby, VIC, Australia) and then air-dried. To determine if there were cells in the waste outlet, DPBS in the waste tube was centrifuged, and cell pellets were deposited onto 4 adhesive glass slides with Cytospin™ 4 Cytocentrifuge for further quantitation. Deposited cells on slides were fixed with ice-cold acetone for 3 min at –20 °C and mounted with a Fluoroshield medium containing 4′,6-diamidino-2-phenylindole (DAPI) Prolong Gold Antifade Reagent(ThermoFisher Scientific, sScoresby, VIC, Australia) covered with a coverslip and counted using a fluorescence microscope (Zeiss Axio Imager Z2 Upright Microscope, North Ryde, NSW, Australia).
3
Lung Tissue and Fluid Sampling
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