The PBMC were incubated with combinations of fluorescein isothiocyanate (FITC), phycoerythrin (PE), PE-cyanine 5.5 (PE-cy5.5), and peridinin chlorophyll protein monoclonal antibodies. The monoclonal antibodies were CD3-FITC, CD3-Percp/Cy5.5, CD4-PE, CD4-FITC, CD8-FITC, CD8-PE, CD16-FITC, CD56-PE, CD19-PE, CD25-FITC, CD127-Percp/Cy5.5, and CD28-PE (Beckman Coulter). About 10,000 lymphocytes were assessed with FC500 software to determine the percentage of CD3+, CD4+, CD8+, CD3−CD16+CD56+, CD19+, CD4+CD25+CD127+, CD8+CD28−, and CD8+CD28+ lymphocytes.
Cd25 fitc
Manufactured by Beckman Coulter
CD25-FITC is a fluorescently-labeled monoclonal antibody that binds to the CD25 antigen, also known as the interleukin-2 receptor alpha chain (IL-2Rα). It is used in flow cytometry applications for the identification and enumeration of CD25-expressing cells.
Automatically generated - may contain errors
Lab products found in correlation
Cd4 pe, by Beckman Coulter (1 mentions)
Cd56 pe, by Beckman Coulter (1 mentions)
Cd28 pe, by Beckman Coulter (1 mentions)
Cd8 pe, by Beckman Coulter (1 mentions)
Cd19 pe, by Beckman Coulter (1 mentions)
Cd16 fitc, by Beckman Coulter (1 mentions)
Cd127 pe, by Beckman Coulter (1 mentions)
Cd4 pc5, by Beckman Coulter (1 mentions)
Cytomics fc500, by Beckman Coulter (1 mentions)
2 protocols using cd25 fitc
1
Quantifying Immune Cell Subsets
2
Flow Cytometric Quantification of CD4+ T Cells
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The CD4 T lymphocytes were assessed in peripheral blood by the method of flow cytometry. Ten μL of monoclonal antibodies CD4-PC5, CD25-FITC, and CD127-PE (Beckman Coulter) were added to each sample with 100 μL of whole blood. After 30 min of incubation at room temperature, each sample was subjected to a rapid, automatic lysis process (ImmunoPrep Work Station, Beckman Coulter). After thorough mixing, the samples were analyzed by the method of flow cytometry (Cytomics FC500, Beckman Coulter) and every 105 cells were counted. The control was determined according to the FMO (fluorescence-minus-one) method.
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