Maleimido mono amide dota
Maleimido-mono-amide-DOTA is a bifunctional metal chelator that can be used to label biomolecules with metal ions for various applications. It contains a maleimide group for covalent conjugation to biomolecules and a DOTA (1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid) chelating group for complexation of metal ions.
Lab products found in correlation
6 protocols using maleimido mono amide dota
PET Imaging of CCR2 and CCR5 Receptors
Mass Cytometry Cell Barcoding Protocol
Single-Cell Palladium Barcoding Protocol
Palladium Isotope Barcoding Protocol
Radiolabeling and Characterization of ECL1i
Characterization of Antibody-Based Therapeutics
and trastuzumab (Herceptin; lot: 19B020E) were available from Chugai
Pharmaceutical Co. Ltd. (Chuo-ku, Tokyo, Japan). Cetuximab (Erbitux;
lot: ERBA012) and mogamulizumab (Poteligeo; lot: 19303P) were purchased
from Merck Biopharma (Meguro-ku, Tokyo, Japan) and Kyowa Hakko Kirin
Co. Ltd. (Chiyoda-ku, Tokyo, Japan), respectively. Payload (dibenzocyclooctyne-PEG4-biotin) was purchased from Sigma-Aldrich (St. Louis, MO,
USA). Bifunctional chelating agents, p-SCN-Bn-DOTA
and maleimido-mono-amide-DOTA, were purchased from Macrocyclics (Dallas,
TX, USA). Sialylglycan and 2-chloro-1,3-dimethyl-1H-benzimidazol-3-ium chloride for preparing oxazoline were from Fushimi
Pharmaceutical Co., Ltd. (Marugame, Kagawa).
A TSKgel FcR-IIIA-NPR
(4.6 mm I.D. × 7.5 cm) column from TOSOH (Minato-ku, Tokyo, Japan)
was used for the analysis of all samples. Protein A column chromatography
was available from TOSOH (Minato-ku, Tokyo, Japan). HPLC (Prominence,
SHIMADZU, Kyoto, Japan) was used for analyses and purification. The
antibody concentration was measured by a NanoDrop Lite (Thermo Fisher
Scientific, Waltham, MA, USA). The 1H-spectra of oxazolines
were measured on a JEOL JMN-ECZL spectrometer (400 MHz) at ambient
temperature in D2O. Sephadex G-25 was purchased from GE
Healthcare (Chicago, IL, USA) for purification of glycan oxazoline.
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