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Ptt a reagent

Manufactured by Diagnostica Stago
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Sourced in France

The PTT-A reagent is a laboratory diagnostic product used to measure the activated partial thromboplastin time (aPTT) in plasma samples. It is designed to assess the intrinsic and common coagulation pathways.

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Lab products found in correlation

Dextran sulfate, by Merck Group (1 mentions) Ellagic acid, by Thermo Fisher Scientific (1 mentions) Argatroban, by GlaxoSmithKline (1 mentions) ε aminocaproic acid εaca, by Merck Group (1 mentions) S2302 h d prolyl l phenylalanyl l arginine p nitroanilide, by Diapharma (1 mentions) S 2366 l pyro glu l pro l arg p nitroanilide, by Diapharma (1 mentions) Soybean trypsin inhibitor, by Merck Group (1 mentions) Polyethyleneimine, by Merck Group (1 mentions) α thrombin, by Enzyme Research (1 mentions) Corn trypsin inhibitor, by Enzyme Research (1 mentions) βfxiia, by Enzyme Research (1 mentions) 96 well flat bottom microtiter plate, by Corning (1 mentions) Amv rt enzyme, by Roche (1 mentions) Ethylene glycol, by Merck Group (1 mentions) St4 coagulation analyzer, by Diagnostica Stago (1 mentions)

3 protocols using ptt a reagent

1

Quantification of FXII and PK Activation

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Materials used were as follows: normal human plasma (Precision BioLogic, Dartmouth, Canada); FXII-deficient plasma (George King Biomed); FXII, FXIIa, βFXIIa, PK, PKa, α-thrombin, corn trypsin inhibitor, and horseradish peroxidase–conjugated goat anti-human FXII and anti-human PK (Enzyme Research Laboratory, South Bend, IN); dextran sulfate (500 000 Da), polyethyleneimine (PEI; 750 000 Da), soybean trypsin inhibitor, and ε-aminocaproic acid (ε-ACA; Sigma-Aldrich, St. Louis, MO); ellagic acid (ACROS Organics, The Hague, Netherlands); S2302 (H-d-prolyl-l-phenylalanyl-l-arginine-p-nitroanilide) and S-2366 (l-pyro-Glu-l-Pro-l-Arg-p-nitroanilide; DiaPharma, Westchester, OH); PTT-A reagent (Diagnostica Stago, Asnieres-sur-Seine, France); argatroban (GlaxoSmithKline, Brentford, United Kingdom); polyphosphate (poly-P; 200-1300 units; provided by James Morrissey, University of Michigan); and horseradish peroxidase–conjugated antihemagglutinin immunoglobulin G (IgG; Invitrogen). Monoclonal IgGs 15H8 and 1B28,18 (link) are described.
Shamanaev A., Ivanov I., Sun M.F., Litvak M., Srivastava P., Mohammed B.M., Shaban R., Maddur A., Verhamme I.M., McCarty O.J., Law R.H, & Gailani D. (2022). Model for surface-dependent factor XII activation: the roles of factor XII heavy chain domains. Blood Advances, 6(10), 3142-3154.
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2

SELEX Aptamer Development for Coagulation Factors

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SELEX DNA templates 5′-TCGGGCGAGTCGTCTG-N40-CCGCATCGTCCTCCCTA-3′ were from Oligos etc. (Wilsonville, OR). 5′ and 3′ SELEX primer sequences, 5′-GGGGGAATTCTAATACGACTCACTATAGGGAGGACGATGCGG-3′ and 5′-TCGGGCGAGTCGTCTG-3′, respectively, were from Integrated DNA Technologies (Coralville, IA). 2′-flouro modified cytidine and uridine were from Trilink BioTechnology (San Diego). FXI, FXIa, factors IX and IXaβ were from Haematologic Technologies (Essex Junction, VT). FXIIa was from Enzyme Research Lab (South Bend, IN). Normal pooled plasma was from George King Biomed (Overland Park, Kansas). PTT-A Reagent for partial thromboplastin time (aPTT) assays was from Diagnostica Stago (Parsippany, NJ). AMV-RT enzyme was from Roche Applied Science (Indianapolis). Bovine serum albumin (BSA) was from EMD Chemicals (Gibbstown, NJ), ethylene glycol was from Sigma-Aldrich (St. Louis) and lima bean trypsin inhibitor was from USB Corporation (Cleveland). Pefachrome FXIa 3107 was from Centerchem, Inc., (Norwalk, CT) and S-2366 was from DiaPharma Group, Inc. (West Chester Township, OH). Kinetic assays were performed in 96-well flat bottom microtiter plates (Corning, Corning, NY).
Woodruff R.S., Ivanov I., Verhamme I.M., Sun M.F., Gailani D, & Sullenger B.A. (2017). Generation and characterization of aptamers targeting factor XIa. Thrombosis research, 156, 134-141.
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3

Quantifying FXI Coagulation Activity

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Thirty microliters of recombinant FXI (0.3–30 nM) in TBS with 0.1% bovine serum albumin (TBSA) was mixed with 30 µL human FXI-deficient plasma and 30 µL PTT-A reagent (Diagnostica Stago, Asnières-sur-Seine, France). After incubation at 37 °C for 5 min, 30 µL of 25 mM CaCl2 was added, and time to clot formation was determined with an ST4 coagulation analyzer (Diagnostica Stago, Asnières-sur-Seine, France). Clotting times were plotted against FXI concentration on log–log plots, and activity of fFXI-M516 as a percentage of fFXI-V516 activity was calculated.
Kuder H., Dickeson S.K., Brooks M.B., Kehl A., Müller E., Gailani D, & Giger U. (2022). A Common Missense Variant Causing Factor XI Deficiency and Increased Bleeding Tendency in Maine Coon Cats. Genes, 13(5), 792.
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