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Rantes

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RANTES is a recombinant human chemokine protein. It is a member of the CC chemokine family and is involved in the recruitment and activation of various immune cells.

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Lab products found in correlation

G csf, by RayBiotech (2 mentions) Specific elisas, by RayBiotech (2 mentions) Mcp 5, by RayBiotech (2 mentions) Mip3α, by RayBiotech (2 mentions) Mip3a, by RayBiotech (2 mentions) Tnf α, by RayBiotech (2 mentions) Epoch microplate spectrophotometer, by Agilent Technologies (1 mentions) Il 1β, by Thermo Fisher Scientific (1 mentions) Interleukin 6 (il 6), by Thermo Fisher Scientific (1 mentions) Interleukin 4 (il 4), by BD (1 mentions) Eotaxin, by BD (1 mentions) Il 13, by R&D Systems (1 mentions) Coating buffer, by Thermo Fisher Scientific (1 mentions) 96 well plate, by BD (1 mentions) Fetal bovine serum (fbs), by Atlanta Biologicals (1 mentions) Tween 20, by Merck Group (1 mentions) Tmb substrate, by BD (1 mentions) Interleukin 6 (il 6), by BD (1 mentions) Elisa ready set go, by Thermo Fisher Scientific (1 mentions) Il 10, by RayBiotech (1 mentions)

Spelling variants of this product

Human RANTES (CCL5) ELISA Kit (2 citations) Rantes quantitative ELISA kits (2 citations)

11 protocols using rantes

1

ELISA Analysis of Inflammatory Factors in BV-2 Cells

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BV-2 cells were treated for 24 h and then supernatants from controls (dH2O only), from cells treated with GLE (0.5 mg/ml) only, LPS only (1.0 μg/ml), and GLE (0.5 mg/ml) + LPS (1.0 μg/ml) (where LPS was added after 1 h incubation with GLE), were used in the assay. Specific ELISAs (RayBiotech, Norcross, GA, USA) were performed using G-CSF (Cat# ELM-G-CSF), IL1α (Cat# ELM-IL1a), MCP-5 (Cat# ELM-MCP5), MIP3α (Cat# ELM-MIP3a), and RANTES (Cat# ELM-RANTES) following manufacturer’s instructions and as previously described (Mendonca et al., 2017 (link)). Briefly, 100 μl of supernatant from samples and standards were added to 96 well plates pre-coated with the capture antibody. After incubation, 100 μl of prepared biotinylated antibody mixture was added to each well and incubated for 1 h. The mixture was then decanted, and streptavidin solution (100 μl) was placed in each well and incubated. Substrate reagent (100 μl) was then added to each well for 30 min, followed by the addition of stop solution (50 μl). Data were quantified by optical density at 450 nm.
Hilliard A., Mendonca P, & Soliman K.F. (2020). Involvement of NFƙB and MAPK signaling pathways in the preventive effects of Ganoderma lucidum on the inflammation of BV-2 microglial cells induced by LPS. Journal of neuroimmunology, 345, 577269.
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2

ELISA-based Cytokine Quantification

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RANTES (RayBiotech), IL‐1β and IL‐6 (eBioscience) concentrations in cell culture media and mouse serum were measured using Enzyme‐linked immunosorbent assay (ELISA) kits according to the manufacturer's instructions. The plates were measured at 450 nmol/L using an Epoch microplate spectrophotometer (BioTek Instruments). The concentrations of RANTES, IL‐1β and IL‐6 in the samples were determined by standard curves.
Jung F., Liu J., Yang S., Tseng H., Chou S.P., Lin J, & Jow G. (2021). FJU‐C28 inhibits the endotoxin‐induced pro‐inflammatory cytokines expression via suppressing JNK, p38 MAPK and NF‐κB signaling pathways. Pharmacology Research & Perspectives, 9(6), e00876.
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3

Quantification of Allergy Biomarkers

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Concentrations of eotaxin, IL-4, IL-5 (BD Pharmingen, CA, USA), RANTES (RayBiotech Inc., GA), and IL-13 (R&D Systems, Minneapolis, MN) in BALF were quantified using sandwich EIA kits according to the manufacturer's instructions. Similarly, the serum level of total IgE was quantified with a commercially available EIA kit (BD Pharmingen, CA, USA).
Rajajendram R., Tham C.L., Akhtar M.N., Sulaiman M.R, & Israf D.A. (2015). Inhibition of Epithelial CC-Family Chemokine Synthesis by the Synthetic Chalcone DMPF-1 via Disruption of NF-κB Nuclear Translocation and Suppression of Experimental Asthma in Mice. Mediators of Inflammation, 2015, 176926.
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4

Cytokine Profiling in Synovial Fluid and Plasma

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Cell-free primary RA synovial fluid and plasma from healthy blood donors were used for detecting IL6 (BD OptEIA), IL8 (BD OptEIA), IL-17 (peptrotech), TNFα (Pierce-endogen) and RANTES (Ray Biotech) by the BD OptEIA enzyme-linked immunosorbent assay (ELISA) Kit following the manufacturers’ protocol. Briefly, non-tissue culture treated 96-well plates (Becton Dickson) were coated with capture antibody diluted in coating buffer (eBioscience) and incubated overnight at 4°C. PBS (Mideatech, Inc.) 10% FBS (Atlanta Biologicals) was used for blocking and as assay diluent as specified in the protocol. All washes during the protocol were performed with a Bio-Rad wash machine (Model 1575 Immunowash) using PBS with 0.5% Tween-20 (Sigma). After adding substrate, a color reagent TMB substrate also from BD biosciences, samples were stopped with the TMB stop solution (Kirkegaard and Perry Labs). Plates were read in a Spectramax PLUS 384 spectrophotometer at 450 nm with a background subtraction wavelength of 570 nm.
Chen X., Eksioglu E.A., Carter J.D., Fortenbery N., Donatelli S.S., Zhou J., Liu J., Yang L., Gilvary D., Djeu J, & Wei S. (2015). Inactivation of DAP12 in PMN Inhibits TREM1-Mediated Activation in Rheumatoid Arthritis. PLoS ONE, 10(2), e0115116.
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5

Quantifying IL-29 and RANTES Levels

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IL-29, ELISA Ready-Set-Go (eBioscience, San Diego, CA) and RANTES (RayBiotech, Norcross, GA) enzyme-linked immunosorbent assays (ELISAs) were performed as per the manufacturer's protocol.
Israelow B., Narbus C.M., Sourisseau M, & Evans M.J. (2014). HepG2 cells mount an effective antiviral interferon-lambda based innate immune response to hepatitis C virus infection. Hepatology (Baltimore, Md.), 60(4), 1170-1179.
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6

Macrophage Response to Tumor Exosomes

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Murine macrophage RAW 264.7 cells were seeded at a density of 104 in each chamber of an 8 well-chambered slide, allowed to adhere overnight, and subsequently treated or left untreated. Treatment regimens were a) 100 ng LPS for 24 hours (positive control), b) untreated 4T1-derived exosomes, c) HT treated 4T1-derived exosomes, d) untreated EMT-6-derived exosomes, and e) HT treated EMT-6-derived exosomes. The exosome-treated sets of cells were exposed to 1×106 vesicles for 24 hours. The RAW 264.7 supernatant was collected for ELISA analysis of IL-10, TNF-α, and RANTES according to the manufacturer’s instructions (RayBiotech, USA).
Sen K., Sheppe A.E., Singh I., Hui W.W., Edelmann M.J, & Rinaldi C. (2020). Exosomes released by breast cancer cells under mild hyperthermic stress possess immunogenic potential and modulate polarization in vitro in macrophages. International journal of hyperthermia : the official journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group, 37(1), 696-710.
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7

Cytokine Secretion Analysis in TNF-α-Treated Cells

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Following 24-hour coincubation with 10 ng/mL TNF-α and increasing concentrations of DMPF-1, spent media was collected and stored at −80°C prior to chemokine immunoassay. The concentrations of MCP-1, IL-8, eotaxin-1 (BD Pharmingen, USA), RANTES, and GRO-α (RayBiotech Inc., GA) were quantified with commercially available sandwich ELISA kits. All assays were conducted according to the manufacturer's instructions.
Rajajendram R., Tham C.L., Akhtar M.N., Sulaiman M.R, & Israf D.A. (2015). Inhibition of Epithelial CC-Family Chemokine Synthesis by the Synthetic Chalcone DMPF-1 via Disruption of NF-κB Nuclear Translocation and Suppression of Experimental Asthma in Mice. Mediators of Inflammation, 2015, 176926.
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8

Anti-CD20 Antibody Modulation of B Cell-Fibroblast Interactions

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The B-lymphocyte depletion effect was mediated by anti-CD20 monoclonal antibody rituximab (RTX, Roche, Shanghai, China). IGF-1R binding protein was used to block the IGF-1R pathway. CpG oligodeoxynucleotides were used to stimulate B lymphocyte activity. An MTS assay (Promega, USA) was used to detect cell proliferation activity in cells treated with different concentrations of RTX (1 mg/mL, 0.8 mg/mL, 0.6 mg/mL, 0.4 mg/mL or 0.2 mg/mL), the IGF-1 binding protein, or CpG. On day 1 of co-culturing orbital fibroblasts with B lymphocytes, 1 mg/mL RTX, 5 µL/mL IGF-1 binding protein, and 1 µg/mL CpG were added to the medium, according to the results of the MTS assay (data not shown). A negative control group was created for each drug treatment. The supernatant of co-cultured cells was collected after 48 h and centrifuged at 1,000 g for 10 min to remove debris. The expression levels of interleukin-6 (IL-6, RayBiotech, USA) and RANTES (RayBiotech, USA) were quantified in triplicate via an enzyme-linked immunosorbent assay (ELISA) according to the manufacturer’s protocols.
Wang R., Song D., Zhong Y, & Li H. (2024). Potential role of IGF-1R in the interaction between orbital fibroblasts and B lymphocytes: an implication for B lymphocyte depletion in the active inflammatory phase of thyroid-associated ophthalmopathy. BMC Immunology, 25, 31.
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9

Cytokine Analysis in Stroke Model

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For cytokine analysis, 2 months old males (6 animals per group) were used. At 1, 4 or 7 days after stroke the animals were anesthetized and their blood flushed out with 20ml cold PBS via transcardial perfusion. Then, the brains were harvested and cut to 1mm thick coronal sections. After a quick TTC staining, infarct core (approximate 2 by 2 mm) was dissected out and frozen with liquid nitrogen. Since we found that the most anterior sections, containing the striatum area, produced the most comparable size between the cortical strokes in each type of model, we limited the cytokine analysis to the first half of the brain. For cytokine analysis, brain tissue was subject to multiplex ELISA analysis through the Quantibody Mouse Cytokine Array for the following cytokines/chemokines: bFGF, BLC, CD30L, eotaxin, eotaxin-2, FasL, G-CSF, GM-CSF, ICAM, IFN-γ, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-10, IL-12p40, IL-13, IL-15, IL-17, IL-21, KC, leptin, LIX, MCP-1, MCP-5, M-CSF, MIG, MIP-1a, MIP-1g, PF-4, RANTES, TARC, TCA-3, TNF RI, TNF RII, TNFα (RayBiotech Inc.).
Cotrina M.L., Lou N., Tome-Garcia J., Goldman J, & Nedergaard M. (2016). DIRECT COMPARISON OF MICROGLIAL DYNAMICS AND INFLAMMATORY PROFILE IN PHOTOTHROMBOTIC AND ARTERIAL OCCLUSION EVOKED STROKE. Neuroscience, 343, 483-494.
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10

Plasma Biomarker Quantification

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Plasma biomarkers were assayed by enzyme-linked immunosorbent assay. Kits were purchased from the following sources: neuropeptide Y (NPY), RANTES (Regulated on activation, normal T-cell-expressed and secreted), and E-selectin from Ray Biotech (Norcross, GA); serotonin from Enzo Life Sciences Inc (Farmingdale, NY); CS846 (aggrecan chondroitin sulfate 846) from IBEX Pharmaceuticals Inc (Montreal, Quebec, Canada); and CTX-II (C-terminal telopeptide of type II collagen) from Immunodiagnosticsystems, IDS Inc (Gaiersburgh, MD). All samples were assayed in duplicate and averaged following the manufactures’ instructions and reported as concentration (ng/ml).
Schaaf S., Huang W., Perera S., Conley Y., Belfer I., Jayabalan P., Tremont K., Coelho P., Ernst S., Cortazzo M., Weiner D., Vo N., Kang J, & Sowa G. (2021). Association of Protein and Genetic Biomarkers With Response to Lumbar Epidural Steroid Injections in Subjects With Axial Low Back Pain. American journal of physical medicine & rehabilitation, 100(1), 48-56.
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