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Creatinine assay kit

Manufactured by R&D Systems
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The Creatinine Assay Kit is a colorimetric assay designed to measure creatinine levels in various biological samples, such as serum, plasma, urine, and cell culture media. The kit utilizes a simple and reliable method to quantify creatinine concentrations accurately.

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Lab products found in correlation

Pierce bca assay, by Thermo Fisher Scientific (3 mentions) Quantichrom urea assay kit, by BioAssay Systems (2 mentions) Bio plex manager 6, by Bio-Rad (1 mentions) Albumin elisa kit, by Fortis Life Sciences (1 mentions) Mki2mag 94k, by Merck Group (1 mentions)

5 protocols using creatinine assay kit

1

Serum and Urine NGAL Analysis in Pigs

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Serum and urine samples were taken immediately prior to euthanasia for measurement of serum NGAL and urine NGAL/creatinine ratios. Pig NGAL ELISA kit (Enzo Life Sciences, Farmingdale, NY) was used to analyze serum and urine samples by a Biotek plate reader (Winooski, VT). Creatinine assay kit (R&D Systems, Minneapolis, MN) was used to determine urine concentrations by Biotek plate reader (Winooski, VT). Serum creatinine was measured using a commercially available DRI-CHEM analyzer (HESKA Lab Systems, Loveland, CO).
Davidson J.A., Khailova L., Treece A., Robison J., Soranno D.E., Jaggers J., Ing R.J., Lawson S, & Lujan S.O. (2019). Alkaline Phosphatase Treatment of Acute Kidney Injury in an Infant Piglet Model of Cardiopulmonary Bypass with Deep Hypothermic Circulatory Arrest. Scientific Reports, 9, 14175.
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2

Urine Protein and Creatinine Quantification

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Urine creatinine levels were measured using Creatinine Assay Kit (R&D Systems, Minneapolis, MN) based on the Jaffe alkaline picrate method and following the manufacturer recommended protocol. Serum urea nitrogen (SUn) was determined using QuantiChrom™ Urea Assay Kit (BioAssay System, Atlanta, GA) following the manufacturer recommended protocol. To measure protein concentration in collected urine samples, pre-cooled 40 μl distilled and deionized water (ddH2O) and 200 μl of pre-chilled acetone (overnight at −20 °C) were added to 10 μL urine. The sample was vigorously mixed and incubated at −20 °C for 30 min, then centrifuged at 14,000 × g 4 °C, for 15 min. Precipitated proteins were resuspended in 40 μl ddH2O and protein content was measured using Pierce BCA assay (Thermo Scientific, Rockford, IL). Protein excretion calculation formula: protein concentration × volume/minutes (4 h); urine output calculation formula: total urine volume/minutes (4 h).
Zhang A., Uaesoontrachoon K., Shaughnessy C., Das J.R., Rayavarapu S., Brown K.J., Ray P.E., Nagaraju K., van den Anker J.N., Hoffman E.P, & Hathout Y. (2015). The use of urinary and kidney SILAM proteomics to monitor kidney response to high dose morpholino oligonucleotides in the mdx mouse. Toxicology Reports, 2, 838-849.
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3

Urine Biomarker Quantification Protocol

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Urine creatinine levels were measured using Creatinine Assay Kit (R&D Systems, Minneapolis, MN) based on the Jaffe alkaline picrate method and following the manufacturer recommended protocol. Serum urea nitrogen (SUn) was determined using QuantiChrom™ Urea Assay Kit (BioAssay System, Atlanta, GA) following the manufacturer recommended protocol. To measure protein concentration in collected urine samples, pre-cooled 40 µl distilled and deionized water (ddH2O) and 200 µl of pre-chilled acetone (overnight at − 20 °C) were added to 10 µL urine. The sample was vigorously mixed and incubated at − 20 °C for 30 min, then centrifuged at 14000 x g 4°C, for 15 minutes. Precipitated proteins were resuspended in 40 µl ddH2O and protein content was measured using Pierce BCA assay (Thermo Scientific, Rockford, IL). Protein excretion calculation formula: protein concentration x volume/ minutes (4 hrs.); urine output calculation formula: total urine volume / minutes (4 hrs.).
Zhang A., Uaesoontrachoon K., Shaughnessy C., Das J.R., Rayavarapu S., Brown K.J., Ray P.E., Nagaraju K., van den Anker J.N., Hoffman E.P, & Hathout Y. (2015). The use of urinary and kidney SILAM proteomics to monitor kidney response to high dose morpholino oligonucleotides in the mdx mouse. Toxicology Reports, 2, 838-849.
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4

Comprehensive Urine Biomarker Profiling

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Urine creatinine was measured using a Creatinine Assay Kit (R&D Systems, Minneapolis, MN), following the manufacturer’s instructions. Total protein in urine samples was measured, as previously described (PMID: 26213685). Briefly, proteins were precipitated from urine samples by adding 40 μL of water and 200 μL of prechilled acetone to 10 μL of urine. Samples were then incubated at −20°C for 30 min and then centrifuged at 14,000 g, 4°C, for 15 min. Pellets were resuspended in 40 μL of water, and protein concentration was measured using the Pierce BCA Assay (Thermo Scientific, Rockford, IL). Albumin from urine samples was measured using the Albumin ELISA Kit (Bethyl Laboratories, Montgomery, TX), following the manufacturer’s instructions.
Acute kidney injury (AKI) biomarker levels were analyzed by multiplex assays, using the Luminex technology. The multiplex kidney injury panels (MKI1MAG-94K, MKI2MAG-94K; Merck Millipore) were used, according to the manufacturer’s instructions, to measure levels of β-2-microglobulin (B2M), KIM-1, vascular endothelial growth factor (VEGF), CysC, epidermal growth factor (EGF), lipocalin-2-NGAL, clusterin, and osteopontin (OPN). The results were read using a Bio-Plex MAGPIX Multiplex Reader and analyzed with Bio-Plex Manager 6.1 software (Bio-Rad, France)
Aupy P., Echevarría L., Relizani K., Zarrouki F., Haeberli A., Komisarski M., Tensorer T., Jouvion G., Svinartchouk F., Garcia L, & Goyenvalle A. (2019). Identifying and Avoiding tcDNA-ASO Sequence-Specific Toxicity for the Development of DMD Exon 51 Skipping Therapy. Molecular Therapy. Nucleic Acids, 19, 371-383.
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5

Rat Urinary Biomarker Quantification

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BG, TG, LDL-C, and BUN levels were measured using an automatic biochemical analyzer at the First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, China. Rat urinary microalbumin was detected using a rat microalbuminuria (MAU/ALB) ELISA Kit (Cusabio) according to the manufacturer’s instructions. Rat urinary creatinine was detected using a creatinine assay kit (R&D Systems China Co., Ltd.), and the UACR was calculated and expressed in mg/g.
Liu H., Wang J., Yue G, & Xu J. (2024). Placenta-derived mesenchymal stem cells protect against diabetic kidney disease by upregulating autophagy-mediated SIRT1/FOXO1 pathway. Renal Failure, 46(1), 2303396.
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