Anti pp38 sc 17852 r

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Anti-Pp38 (sc-17852-R) is a rabbit polyclonal antibody that recognizes the Pp38 protein. It is intended for use in Western Blotting applications.

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Sc-17852-R (4 citations)

3 protocols using anti pp38 sc 17852 r

Phospho-protein Profiling of Cell Signaling

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After activation, cells were lysed and phospho-protein levels were compared using human phospho-kinase antibody array (R&D Systems), according to manufacturer guidelines. Array dots were digitally analyzed using the open-source software ImageJ version 1.49 (NIH, USA). Lysate proteins were also denatured and separated on SDS-PAGE, and transferred to PVDF membrane. Western blotting was performed using primary rabbit antibodies anti-Pp38 (sc-17852-R, Santa Cruz Biotechnology), anti-p38 (#9212), anti-PSAPK/JNK (#9251), anti-SAPK/JNK (#9252), anti-PErk1/2 (#9101), anti-Erk1/2 (#9102), all from Cell Signaling Technology (USA) and mouse anti-actin (MAB1501, Merk Millipore, Germany). To detect primary antibody binding, blots were incubated with horseradish-peroxidase-conjugated anti-rabbit or anti-mouse antibodies. The immobilized antibodies were detected by ECL reagent (Advansta, USA).

Kaner Z., Engelman R., Schuster R., Rider P., Greenberg D., Av-Gay Y., Benhar M, & Lewis E.C. (2019). S-Nitrosylation of α1-Antitrypsin Triggers Macrophages Toward Inflammatory Phenotype and Enhances Intra-Cellular Bacteria Elimination. Frontiers in Immunology, 10, 590.

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Protein Expression Analysis in Sensorimotor Cortex

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Total protein was extracted from sensorimotor cortical tissues and protein concentration was determined as detailed above. Equal amounts of protein (50 µg) were separated by 12% SDS-PAGE followed by transferring onto polyvinylidene difluoride membranes. Polyvinylidene difluoride membranes were blocked with 5% skimmed milk in 0.01% TBS-Tween 20 for 1 h at 37°C and then incubated with anti-BDNF (sc-20981; 1:200; Santa Cruz Biotechnology, Inc., Dallas, TX, USA), anti-phosphorylated (p)-TrkB (4619; 1:500; Cell Signaling Technology, Inc., Danvers, MA, USA), anti-p-p38 (sc-17852-R; 1:4,000; Santa Cruz Biotechnology, Inc.), and anti-GAPDH (sc-51631; 1:5,000; Santa Cruz Biotechnology, Inc.) overnight at 4°C. Membranes were washed with TBST for 15 min and incubated with horseradish peroxidase conjugated goat anti-rabbit immunoglobulin G (cat. no. sc-2004; Santa Cruz Biotechnology, Inc.) for 1 h at 37°C. Membranes were analyzed using an electrochemiluminescence (ECL) reagent and measured using Image Lab 3.0 (Bio-Rad Laboratories, Inc.).

Liang J., Deng G, & Huang H. (2018). The activation of BDNF reduced inflammation in a spinal cord injury model by TrkB/p38 MAPK signaling. Experimental and Therapeutic Medicine, 17(3), 1688-1696.

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Signaling Pathway Modulation Assay

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Clofazimine was purchased from Sigma-Aldrich; TBI-166 was provided by the Institute of Materia Medica, Peking Union Medical College, and Chinese Academy of Medical Sciences; lipopolysaccharides from Escherichia coli O55:B5 (L2880) was purchased from Sigma-Aldrich. BAY11-7082 (M2040), SP600125 (M2076), and U0126 (M1977) were purchased from Abmole. Luciferase reporter assay plasmids for pRL-TK, Gal4-Elk, Gal4-Luc, pFA-cJun, RacL61, pNF-κB-luc, and RasV12 were kindly provided by Cuihua Liu (Institute of Microbiology, Chinese Academy of Sciences). The following antibodies were used in this study: anti-p-Jnk (sc-81502, Santa Cruz, USA), anti-Jnk (9252, CST, USA), anti-p-p38 (sc-17852-R, Santa Cruz, USA), anti-p38 (sc-7972, Santa Cruz, USA), anti-p-IκBα (sc-101713, Santa Cruz, USA), anti-IκBα (sc-371; Santa Cruz, USA), p-ERK1/2 (9101, CST, USA), and ERK1/2 (9102, CST, USA).

Li X., Luo X., Wang B., Fu L., Chen X, & Lu Y. (2024). Clofazimine inhibits innate immunity against Mycobacterium tuberculosis by NF-κB. mSphere, 9(8).

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