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Astemizole

Manufactured by Bio-Techne
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Sourced in United Kingdom, United States

Astemizole is a selective histamine H1 receptor antagonist. It is a laboratory reagent used in research applications.

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Lab products found in correlation

Lipofectamine 3000, by Thermo Fisher Scientific (2 mentions) Normal mouse igg, by Merck Group (1 mentions) Normal rabbit igg, by Merck Group (1 mentions) Epz 5687, by Selleck Chemicals (1 mentions) Eed226, by Selleck Chemicals (1 mentions) Epz 6438, by Selleck Chemicals (1 mentions) H3k27me3, by Cell Signaling Technology (1 mentions) Flag tag (flag), by Cell Signaling Technology (1 mentions) Lc3a b, by Cell Signaling Technology (1 mentions) β actin, by Merck Group (1 mentions) Gapdh, by Santa Cruz Biotechnology (1 mentions) Pgf2α, by Bio-Techne (1 mentions) Histamine, by Merck Group (1 mentions) Pacap 6 38, by Merck Group (1 mentions) Pacap, by Bio-Techne (1 mentions) Adrenomedullin, by Merck Group (1 mentions) Olcegepant, by Bio-Techne (1 mentions) Lisinopril, by Fujifilm (1 mentions) Gentamycin, by Fujifilm (1 mentions) Mibefradil, by Merck Group (1 mentions)

3 protocols using astemizole

1

Epigenetic Modulators in Cancer Research

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GSK126 (406,228, MedKoo), EPZ5687 (S7004, Selleckchem), EPZ6438 (S7128, Selleckchem), EED226 (S8496, Selleckchem) and astemizole (3,489, Tocris) were dissolved in 100% ethanol or DMSO for cell treatment. Lipofectamine 3,000 (Thermo Fisher Scientific) was used to perform the transfection of EZH2 shRNA and EED shRNA (Sigma). The following antibodies were used: AR (06–680, Millipore), EZH2 (5,246, Cell Signaling), rabbit polyclonal anti-EED (09–774, Millipore), mouse monoclonal anti-EED (05–1,320, Millipore), normal rabbit IgG (12–370, Millipore), normal mouse IgG (12–371, Millipore), GST (sc-138, Santa Cruz), FLAG (14,793, Cell Signaling), PSA (A0562, Dako), GAPDH (sc-32,233, Santa Cruz), H3K27me3 (9,733, Cell Signaling), H3 (9,715, Cell signaling), β-Actin (A2228, Sigma), LC3-A/B (12,741, Cell Signaling).
Liu Q., Wang G., Li Q., Jiang W., Kim J.S., Wang R., Zhu S., Wang X., Yan L., Yi Y., Zhang L., Meng Q., Li C., Zhao D., Qiao Y., Li Y., Gursel D.B., Chinnaiyan A.M., Chen K, & Cao Q. (2019). Polycomb group proteins EZH2 and EED directly regulate androgen receptor in advanced prostate cancer. International journal of cancer, 145(2), 415-426.
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2

Molecular Mediators in Vascular Regulation

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CGRP, amylin, PACAP, VIP, PGF2α, olcegepant, astemizole, ER819762 and Ro1138452 were from Tocris Bioscience (Bristol, UK); adrenomedullin, PACAP6-38, PGE2, PGI2 and histamine were from Sigma Aldrich (Milan, Italy); the mouse monoclonal anti-CGRP antibody (clone [4901]) and the inactive immunoglobulin (mouse monoclonal IgG2a) were from Abcam (Cambridge, UK)
De Logu F., Landini L., Janal M.N., Li Puma S., De Cesaris F., Geppetti P, & Nassini R. (2019). Migraine-provoking substances evoke periorbital allodynia in mice. The Journal of Headache and Pain, 20(1), 18.
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3

Drug Compound Sourcing and Preparation

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Ambrisentan, amlodipine, bisoprolol, candesartan, linagliptin, naftopidil, prasugrel, and sumatriptan were purchased from Selleck Chemicals (Houston, TX, USA). Clonidine, glyburide, lisinopril, nicorandil, and propafenone were obtained from Wako (Tokyo, Japan). Efonidipine, mibefradil, terfenadine, and terodiline were provided by Sigma-Aldrich (St. Louis, MO, USA). Aliskiren and warfarin were from LKT Laboratories (St. Paul, MN, USA); astemizole and bepridil were from Tocris Bioscience (Bristol, UK); amiodarone and atorvastatin were from Toronto Research Chemicals (Toronto, Canada); cisapride was from Abcam (Cambridge, UK); and ketotifen was from Tokyo Chemical Industry (Tokyo, Japan). All compounds were dissolved in a mixture of dimethyl sulfoxide (DMSO) and methanol (7:3), and were stored at -80°C. For cell culture, gentamycin and gelatin (type A, cell culture tested) were obtained from Wako and Sigma-Aldrich, respectively.
Higa A., Hoshi H., Yanagisawa Y., Ito E., Morisawa G., Imai J.I., Watanabe S, & Takagi M. (2017). Evaluation system for arrhythmogenic potential of drugs using human-induced pluripotent stem cell-derived cardiomyocytes and gene expression analysis. The Journal of toxicological sciences, 42(6).
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