Fc receptors were blocked with anti-mouse CD16/32 (BioLegend, clone: 93), and surface markers were stained with BV421-conjugated anti-CD3 (BioLegend, clone: 145-2C11), FITC-conjugated anti-CD4 (BioLegend, clone: RM4-5), Alexa Fluor 647-conjugated anti-CD11b (BioLegend, clone: M1/70), PE-conjugated anti-Gr1 (BioLegend, clone: RB6-8C5), PE-conjugated anti-CD44 (BioLegend, clone: IM7), PerCP/Cy5.5-conjugated anti-CD62L (BioLegend, clone: MEL-14), and APC/Cy7-conjugated anti-CD25 (BioLegend, clone: 3C7). After fixing and perminization, intracellular molecules, including cytokines and transcription factors, were stained with PE/Cy7-IL-17A (BioLegend, clone: TC11-18H10.1), PerCP/Cy5.5-conjugated anti-RORγt (BD, clone: Q31-378), Alexa Fluor 647-conjugated anti-FOXP3 (BioLegend, clone:150D), PerCP/Cy5.5-conjugated anti-GM-CSF (BioLegend, clone: MP1-22E9), and Alexa Fluor 647-conjugated anti-TNF-α (BioLegend, clone: MP6-XT22).
Percp cy5.5 conjugated anti rorγt
Manufactured by BD
PerCP/Cy5.5-conjugated anti-RORγt is a fluorescently labeled antibody that binds to the retinoid-related orphan receptor gamma t (RORγt) protein. RORγt is a transcription factor that plays a key role in the development and function of T helper 17 (Th17) cells. The PerCP/Cy5.5 fluorescent label allows for the detection and analysis of RORγt-expressing cells using flow cytometry or other fluorescence-based techniques.
Automatically generated - may contain errors
Lab products found in correlation
Bv421 conjugated anti cd3, by BioLegend (2 mentions)
Fitc conjugated anti cd4, by BioLegend (2 mentions)
Anti mouse cd16 32, by BioLegend (2 mentions)
Apc cy7 conjugated anti cd25, by BioLegend (2 mentions)
Il 17a pe cy7, by BioLegend (2 mentions)
Alexa fluor 647 conjugated anti foxp3, by BioLegend (2 mentions)
Pe conjugated anti cd44, by BioLegend (1 mentions)
2 protocols using percp cy5.5 conjugated anti rorγt
1
Multiplex Immune Cell Phenotyping
2
Multicolor Flow Cytometry for T Cell Phenotyping
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Anti-mouse CD16/32 (BioLegend, clone: 93) was used to block Fc receptors, and the surface markers were stained the following antibodies: FITC-conjugated anti-CD4 (BioLegend, clone: RM4-5), BV421-conjugated anti-CD3 (BioLegend, clone: 145-2C11), and APC/Cy7-conjugated anti-CD25 (BioLegend, clone: 3C7). After fix and permeabilization, cytokines and transcription factors were stained with the following antibodies: PerCP/Cy5.5-conjugated anti-RORγt (BD Biosciences, clone: Q31-378), PE/Cy7-IL-17A (BioLegend, clone: TC11-18H10.1), and Alexa Fluor 647-conjugated anti-FOXP3 (BioLegend, clone: 150D).
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