Cy5 light cube

Manufactured by Thermo Fisher Scientific

The Cy5 light cube is a component used in fluorescence imaging and detection applications. It provides an excitation light source and emission filter optimized for the Cy5 fluorescent dye. The core function of the Cy5 light cube is to facilitate the detection and analysis of samples labeled with Cy5 fluorophores.

Automatically generated - may contain errors

Lab products found in correlation

Evos fl imaging system, by Thermo Fisher Scientific (2 mentions) Vectashield, by Vector Laboratories (1 mentions)

3 protocols using cy5 light cube

Visualization of Recombinant Human LCMV Glycoprotein Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols

10ml of 293F cells at a density of 1 × 10⁶ cells/ml were transfected with equal amounts of pCAGGS-rhLCVgH, pCAGGS-rhLCVgL,⁶¹ (link) or mock transfected using 293 Free according to the manufacturer’s instructions. 1 hour following transfection 500μl of cell culture was added to 500μl of DMEM in a 6-well dish. 24 hours later the cells were fixed in 10% formalin washed 3X with PBS containing 1% BSA and 0.1% Tween-20. The cells were then incubated with 1μg of AMMO1 conjugated to Dylite-650 in 1ml of PBS containing 1% BSA and 0.1% Tween-20 at room temperature for 30min. The cells were then washed 3 times with PBS and visualized on an EvosFL imaging system using a 20 × objective lens and a Cy5 light cube (ThermoFisher).

Singh S., Homad L.J., Akins N.R., Stoffers C.M., Lackhar S., Malhi H., Wan Y.H., Rawlings D.J, & McGuire A.T. (2020). Neutralizing Antibodies Protect against Oral Transmission of Lymphocryptovirus. Cell Reports Medicine, 1(3), 100033.

+ Open protocol

+ Expand

Visualizing LCMV Glycoprotein Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols

10ml of 293F cells at a density of 1 × 10⁶ cells/ml were transfected with equal amounts of pCAGGS-rhLCVgH, pCAGGS-rhLCVgL,^{61 (link)} or mock transfected using 293 Free according to the manufacturer’s instructions. 1 hour following transfection 500μl of cell culture was added to 500μl of DMEM in a 6-well dish. 24 hours later the cells were fixed in 10% formalin washed 3X with PBS containing 1% BSA and 0.1% Tween-20. The cells were then incubated with 1μg of AMMO1 conjugated to Dylite-650 in 1ml of PBS containing 1% BSA and 0.1% Tween-20 at room temperature for 30min. The cells were then washed 3 times with PBS and visualized on an EvosFL imaging system using a 20 × objective lens and a Cy5 light cube (ThermoFisher).

+ Open protocol

+ Expand

Strand-specific RNA FISH for Xist Visualization

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Strand-specific RNA FISH was performed with fluorescently labelled oligonucleotides (IDT) as described previously^{103 (link)}. Briefly, cells were fixed with 4% paraformaldehyde for 10 min at room temperature and then permeabilized for 5 min on ice in 0.5% Triton-X. 10 ng/ml equimolar amounts of Cy5 labelled Xist probes BD384-Xist-Cy5-3’-AM (5’-ATG ACT CTG GAA GTC AGT ATG GAG /3Cy5Sp/ -3’) and BD417-5’Cy5-Xist-Cy5-3’-AM (5’- /5Cy5/ATG GGC ACT GCA TTT TAG CAA TA /3Cy5Sp/ -3’) were hybridized in 40% formamide, 10% dextran sulfate, 2xSSC pH 7 at room temperature overnight. Slides were then washed in 30% formamide 2xSSC pH 7 at room temperature, followed by washes in 2xSSC pH 7 and then mounted with Vectashield (Vector Laboratories, H1200). Images were acquired using an EVOS and a Cy5 light cube (Thermo Fisher Scientific).

Bauer M., Vidal E., Zorita E., Üresin N., Pinter S.F., Filion G.J, & Payer B. (2021). Chromosome compartments on the inactive X guide TAD formation independently of transcription during X-reactivation. Nature Communications, 12, 3499.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!