Log In Sign up
The largest database of trusted experimental protocols

Isoproterenol

Manufactured by Bio-Techne
Visit Supplier
Sourced in United Kingdom, Spain

Isoproterenol is a laboratory reagent used as a non-selective beta-adrenergic agonist. It is commonly used in research applications to study the effects of beta-adrenergic receptor activation.

Automatically generated - may contain errors

Lab products found in correlation

Propranolol, by Bio-Techne (5 mentions) Forskolin, by Bio-Techne (3 mentions) Phenylephrine, by Bio-Techne (2 mentions) Carbachol, by Merck Group (2 mentions) 3 isobutyl 1 methyl xanthine (ibmx), by Bio-Techne (2 mentions) Celltrace calcein violet, by Thermo Fisher Scientific (2 mentions) Mouse catalog i9646, by Merck Group (2 mentions) Human catalog i1395 il 6, by Merck Group (2 mentions) Propranolol catalog p0884, by Merck Group (2 mentions) Isoproterenol catalog i6504, by Merck Group (2 mentions) Chloroquine, by Enzo Life Sciences (2 mentions) Cyto id autophagy detection kit 2, by Enzo Life Sciences (2 mentions) Etomoxir, by Bio-Techne (2 mentions) Mouse catalog 713704 and human catalog 713604 gm csf, by BioLegend (2 mentions) Mouse catalog 713502 and human catalog 713402 g csf, by BioLegend (2 mentions) Torin 1, by Cell Signaling Technology (2 mentions) Ici 118 551, by Bio-Techne (2 mentions) Atenolol, by Bio-Techne (2 mentions) Acetylcholine ach, by Merck Group (2 mentions) Labchart, by ADInstruments (2 mentions)

Spelling variants of this product

Isoproterenol hydrochloride (5 citations)

30 protocols using isoproterenol

1

Pharmacological Modulation of Neuronal Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Reagents were purchased from Sigma except for GABAzine (SR95531, Tocris#1262), CGP55845 (Tocris#1248), D-AP5 (Tocris#0106), DNQX (Tocris#2312), Noradrenaline (Abcam#ab120717), HEAT (Tocris#0535), Phenylephrine (Tocris#2838), Isoproterenol (Tocris#1747), Win (Tocris#1038), AM251 (Tocris#1117), Rimonabant (Tocris#0923), MPEP (Tocris#1212), CPCCOEt (Tocris#1028), CNO (Hello Bio#HB6149), Cy5-streptavidin (ThermoFisher#434316). For i.p. injection, Rimonabant (Tocris #0923) was first prepared 30 mg/mL stock in DMSO, then diluted in saline with 1% DMSO, 1% Cremophor, 1% Ethanol to make 0.3 mg/mL for injection, finally injected 0.01 mL/g to reach 3 mg/kg injection. Phenylephrine (Tocris #2838) was prepared at 10 mM stock solution in water, then diluted in saline for injection. CNO (HelloBio #HB6149) was prepared 0.1 mg/mL in saline and injected 0.01 mL/1 g to reach 1 mg/kg injection. For slice recordings, all stocks were diluted to final concentrations in ACSF to achieve a final solvent concentration of less than 0.1% v/v
Yang B., Sanches-Padilla J., Kondapalli J., Morison S.L., Delpire E., Awatramani R, & Surmeier D.J. (2021). Locus coeruleus anchors a trisynaptic circuit controlling fear-induced suppression of feeding. Neuron, 109(5), 823-838.e6.
+ Open protocol
+ Expand
2

Carvedilol Formulation Development

Check if the same lab product or an alternative is used in the 5 most similar protocols
Carvedilol was purchased from TCI America (Portland, OR). Tween-80, Polyethylene glycol 400 (PEG 400), methanol, chloroform and phosphate-buffered saline (PBS) tablets were purchased from VWR (Radnor, PA). L-α-phosphatidylcholine (Soy PC or SPC) was purchased from Avanti Polar Lipids, Inc. (Alabaster, AL). Isoproterenol was purchased from Tocris (Minneapolis, MN). Carbopol 934 was purchased from SERVA Electrophoresis GmbH (Heidelberg, Germany). Triethanolamine (TEA) was purchased from Sigma-Aldrich.
Shamim M.A., Yeung S., Shahid A., Chen M., Wang J., Desai P., Parsa C., Orlando R., Meyskens FL J.r., Kelly K.M., Andresen B.T, & Huang Y. (2021). Topical carvedilol delivery prevents UV-induced skin cancer with negligible systemic absorption. International journal of pharmaceutics, 611, 121302.
+ Open protocol
+ Expand
3

Characterization of Signaling Pathways

Check if the same lab product or an alternative is used in the 5 most similar protocols
Isoproterenol, formoterol, GO6983, GO6976, RO32–0432, GF109203X, H89, PSB603, ESI-09, TPPB, NECA (adenosine-5′-N-ethyluronamide), MRS5698 and MRS2365 ([[(1R,2R,3S,4R,5S)-4-[6-amino-2-(methylthio)-9H-purin-9-yl]-2,3-dihydroxybicyclo[3.1.0]hex-1-yl]methyl] diphosphoric acid monoester trisodium salt) were purchased from Tocris (Ellisville, MO). CGS21680">CGS21680, carbachol, cholera toxin (CTX) and PTX were from Sigma (St. Louis, MO). YM254890, enzastaurin, LY333531, VTX-27 and AB928 were purchased from MedChemExpress (Monmouth Junction, NJ, USA). BAY60-6583 (LUF6210, termed hereafter ‘BAY’) was synthesized at Leiden/Amsterdam Center for Drug Research and was provided by Ad IJzerman (Leiden, The Netherlands). All compounds were dissolved in DMSO except that CTX and PTX were in water, and proper controls were included in all experiments. AlphaScreen cAMP kit, SureFire p-ERK1/2 (Thr202/Tyr204) Assay Kit and AlphaScreen SureFire p-Akt 1/2/3 (p-Ser473) Assay Kit were purchased from PerkinElmer (Waltham, MA). Gs-null and Gq/11-null HEK293 cells were generated at Tohoku University, Sendai, Japan. HEK293 human embryonic kidney, PC-3 human prostate cancer, NIH-3T3 mouse fibroblast, and H9C2 rat cardiomyoblast cells were from ATCC (Manassas, VA); all other reagents were from standard commercial sources and of analytical grade.
Gao Z.G., Levitan I.M., Inoue A., Wei Q, & Jacobson K.A. (2023). A2B adenosine receptor activation and modulation by protein kinase C. iScience, 26(7), 107178.
+ Open protocol
+ Expand
4

Mitochondrial Dynamics in Adipocyte Metabolism

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following reagents were used: oligomycin, FCCP, rotenone, antimycin A, ADP, GDP, isoproterenol, CL 316,243 (Tocris Bioscience), isobutylmethylxanthine, dexamethasone, insulin, rosiglitazone, T3, recombinant mouse IL10 (Calbiochem, Cat# 407700), RNeasy (Qiagen), Lipofectamine 3000 (Life Technologies). DNA vectors: pDsRed2-Mito Vector (Clonetech). Antibodies as follows: anti-UCP-1 (Abcam, ab10983), anti- Phospho-(Ser/Thr) PKA Substrate (Cell Signaling #9621), anti-OPA1 (Abcam, ab42364), anti-mitofusin-2 (Abcam, ab 50843), anti-alpha-tubulin (Abcam, ab4074), anti- mtTFA ((A-17): sc-23588), anti-IL10 (Santa Cruz, sc-8438) and anti-OXPHOS cocktail (Abcam, ab110413).
de-Lima-Júnior J.C., Souza G.F., Moura-Assis A., Gaspar R.S., Gaspar J.M., Rocha A.L., Ferrucci D.L., Lima T.I., Victório S.C., Bonfante I.L., Cavaglieri C.R., Pareja J.C., Brunetto S.Q., Ramos C.D., Geloneze B., Mori M.A., Silveira L.R., Segundo G.R., Ropelle E.R, & Velloso L.A. (2018). Abnormal brown adipose tissue mitochondrial structure and function in IL10 deficiency. EBioMedicine, 39, 436-447.
+ Open protocol
+ Expand
5

Pharmacological Modulation of Noradrenergic Pathways

Check if the same lab product or an alternative is used in the 5 most similar protocols
The β-adrenoreceptor antagonist propranolol (2 µg) and β-adrenoreceptor agonist isoproterenol (20 µg) (Tocris Bioscience, UK), or vehicle (0.9% NaCl) were injected via the ipsilateral i.c.v. via the implanted cannula in a 5-µL volume over 5 min, 25 min before LC or PP stimulation. We used these concentrations as there is evidence that they do not alter basal synaptic transmission (Kemp and Manahan-Vaughan 2008a (link)). The half-life of isoprotenerol is only some minutes, whereas the half-life of propranolol is about 2–3 h (Smits and Struyker-Boudier 1979 ; Hadwiger et al. 1997 (link)).
Hansen N, & Manahan-Vaughan D. (2014). Locus Coeruleus Stimulation Facilitates Long-Term Depression in the Dentate Gyrus That Requires Activation of β-Adrenergic Receptors. Cerebral Cortex (New York, NY), 25(7), 1889-1896.
+ Open protocol
+ Expand
6

Pharmacological Agents in Tissue Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols
Resversatrol, noradrenaline, tyramine, isoproterenol, IBMX, lidocaine and diltiazem were obtained from Tocris Bioscience (Madrid, Spain) and dimethyl sulphoxide (DMSO) from Probus, (Barcelona, Spain).
Resveratrol and IBMX were dissolved in DMSO and Tyrode solution (20% DMSO in Tyrode) and noradrenaline, tyramine, isoproterenol, lidocaine and diltiazem were dissolved in Tyrode solution. This stock solution was diluted in a pre-warmed and pre-aerated bathing solution to achieve the final concentration desired. The drug was added to the organ bath at an appropriate concentration so that the concentration of DMSO in the test solution was less than 0.3%, which did not affect any of the preparations.
, & Hernandez-Cascales J. (2017). Resveratrol enhances the inotropic effect but inhibits the proarrhythmic effect of sympathomimetic agents in rat myocardium. PeerJ, 5, e3113.
+ Open protocol
+ Expand
7

Pharmacological Modulation of Cellular Processes

Check if the same lab product or an alternative is used in the 5 most similar protocols
Drugs were applied via bath perfusion, with the final concentrations in the brackets: forskolin (50 μM), H89 (10 μM), isoproterenol (1 μM), CGS21680 (1 μM), and SKF81297 (1 μM) were from Tocris Bioscience; nigericin (5 μM) was from Sigma. The specified concentration of chemicals were either spiked into the circulating buffer, or premade buffers with the correct drug concentrations were switched from one to another via a custom-made solution exchanger.
Chen Y., Saulnier J.L., Yellen G, & Sabatini B.L. (2014). A PKA activity sensor for quantitative analysis of endogenous GPCR signaling via 2-photon FRET-FLIM imaging. Frontiers in Pharmacology, 5, 56.
+ Open protocol
+ Expand
8

Isoproterenol-Induced Cardiac Hypertrophy

Check if the same lab product or an alternative is used in the 5 most similar protocols
Animal experiments were performed according to local and national ethics guidelines. Experimental mice were maintained on an ad libitum diet with free access to water under a standard 12-h light/12-h dark cycle. Here, 12-week-old C57BL6/J male mice were subjected to a daily subcutaneous dose of isoproterenol 50 mg/kg/day (Tocris #1747) for a period of 4, 8, and 11 days; as a control, few mice were given saline doses for a similar period. For the experiments, each group had three or more mice. Mice were euthanized by cervical dislocation, and hearts were perfused with 30 mM KCl to induce diastolic arrest, followed by perfusion with PBS (phosphate-buffered saline). A cross section of each heart was collected in 4% paraformaldehyde (PFA) for histology, and a small amount was snap-frozen for RNA isolation.
Nanda D., Pant P., Machha P., Sowpati D.T, & Kumarswamy R. (2023). Transcriptional changes during isoproterenol-induced cardiac fibrosis in mice. Frontiers in Molecular Biosciences, 10, 1263913.
+ Open protocol
+ Expand
9

Transfection and Receptor Activation Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols
Polyethylenimine (PEI), Linear (MW 25,000) was purchased from Polysciences, Inc. Ropinirole was purchased from BetaPharma (Shanghai) Co. Ltd. DAMGO ((D-Ala2, N-Me-Phe4, Gly-ol5)-enkephalin) was purchased from Mimotopes. SKF83822, neurotensin residues 8-13 (NT8-13), (−)-quinpirole hydrochloride (#1061), acetylcholine chloride (#A2661), carbachol (C4382), D-glucose (#G8270) and pertussis toxin (PTX) were purchased from Sigma-Aldrich. Isoproterenol (#1747) and endothelin-1 (#1160) were purchased from Tocris Bioscience (Bristol, UK). Coelenterazine-h was purchased from both NanoLight™ Technology and Dalton research molecules (#50303-86-9). Forskolin was purchased from Cayman Chemicals (#11018). Nano-Glo® luciferase assay system, containing the furimazine substrate, was purchased from Promega.
Keen A.C., Pedersen M.H., Lemel L., Scott D.J., Canals M., Littler D.R., Beddoe T., Ono Y., Shi L., Inoue A., Javitch J.A, & Lane J.R. (2022). OZITX, a pertussis toxin-like protein for occluding inhibitory G protein signalling including Gαz. Communications Biology, 5, 256.
+ Open protocol
+ Expand
10

Adrenoceptor Agonists and Antagonists

Check if the same lab product or an alternative is used in the 5 most similar protocols
We used prazosin hydrochloride (1 mg/kg) (Wako Pure Chemical Industries Ltd., Osaka, Japan) and propranolol hydrochloride (1 or 10 mg/kg) (TOKYO KASEI, Tokyo, Japan) as adrenoceptor antagonists. Phenylephrine (5 mg/kg) (Wako Pure Chemical Industries Ltd, Osaka, Japan) and isoproterenol (5 mg/kg) (Tocris Bioscience, Bristol, United Kingdom) were used as α receptor and β receptor agonists, respectively. Pilocarpine was mixed with 1X PBS and used as a control treatment.
Wada M., Orihara K., Kamagata M., Hama K., Sasaki H., Haraguchi A., Miyakawa H., Nakao A, & Shibata S. (2017). Circadian clock-dependent increase in salivary IgA secretion modulated by sympathetic receptor activation in mice. Scientific Reports, 7, 8802.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!