Bvt948

UNC0379, NSC663284, BVT948, and Ryuvidine were purchased from Cayman Chemical as candidate chemical agents that stabilize the UHRF1 protein. For synchronization at the G₁/S border, HEK293 cells (ATCC) were treated with 1 μg/mL aphidicolin for 6 hours followed by treatment with 1 μg/mL aphidicolin with or without 1 μM of the indicated chemical agent for 16 hours. For synchronization in the G₂/M phase, cells were treated with 50 ng/mL nocodazole with or without 1 μM of the indicated chemical agent for 16 hours.

The ReFRAME drug repurposing library was provided by Calibr at Scripps Research (La Jolla, CA). The Pandemic Response Box and COVID Box libraries were provided by the Medicines for Malaria Venture (Geneva, Switzerland). The 5’6-FAM/dArUdAdA/3’-TAMRA oligonucleotide was purchased from IDT DNA Inc (Coralville, IA). The compounds DA-3003-1, Ceftazidime, β-lapachone, and BVT-948 were purchased from Cayman Chemical (Ann Arbor, MI). The compounds Exebryl-1 and BN-82685 were synthesized by Los Alamos National Laboratory (Ryszard Michalczyk). The route of chemical synthesis for Exebryl-1 is outlined in S4 Fig.

NovoRapid^® insulin aspart 100 units/mL (Novo Nordisk, Bagsværd, Denmark), sotrastaurin (Item No. 16726, Cayman Chemical, Ann Arbor, MI, USA) and (R)-(+)-etomoxir sodium salt (Cat. No. 4539, Tocris Bioscience) were diluted before use in sterile filtered PBS (137 mM NaCl, 2.7 mM KCl (cat. P9541, Merck KGaA), 10 mM Na₂HPO₄ (cat. 12695147, ACROS Organics™), 1.8 mM KH₂PO₄ (cat. 205920025, ACROS Organics™), pH 7.4). BVT948 (Item No. 16615, Cayman Chemical) and wortmannin (Cat. No. 1232, Tocris Bioscience) were dissolved in dimethyl sulfoxide (DMSO) (cat. D8418, Merck KGaA). BMOV was synthesized using the method previously described by Caravan el al. [48 (link)]