2,2′-Azobis (2-methylpropionamidine) dihydrochloride (AAPH) and 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox) were purchased from Sigma-Aldrich (St. Louis, MO, USA). The synthetic peptide, PHP (Fig. 1A ), with 99% purity, was purchased from GL Biochem Ltd. (Shanghai, China). Microscale MDA, SOD, CAT, GSH-Px, and bicinchoninic acid (BCA) protein assay kits were purchased from Nanjing Jiancheng Institute of Biotechnology (Nanjing, China). ROS and GSH/GSSG assay kits were purchased from Beyotime Institute of Biotechnology (Shanghai, China). Cell counting kit (CCK-8) assay was obtained from Dojindo (Kumamoto, Japan). Trizol reagent was from Life Technologies (USA). Anti-CAT (GTX110704) was purchased from GenTex Inc. (USA). Anti-SOD (Ab13533), anti-GSH-Px (Ab22604), anti-Nrf2 (Ab31163) and anti-Keap1 (Ab218815) were purchased from Abcam (Cambridge, UK). Anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH, AB-P-R001) was from JK GREEN Company (Beijing, China).
Bicinchoninic acid bca protein assay kit
Manufactured by Nanjing Jiancheng
Sourced in China
The Bicinchoninic acid (BCA) protein assay kit is a colorimetric detection and quantitation method used to measure the total protein concentration in a sample. The kit utilizes the combination of the biuret reaction and the highly sensitive and selective detection of the cuprous cation (Cu+) by bicinchoninic acid.
Automatically generated - may contain errors
Lab products found in correlation
Phosphate buffered saline (pbs), by Merck Group (2 mentions)
6 hydroxy 2 5 7 8 tetramethylchroman 2 carboxylic acid, by Merck Group (1 mentions)
Ros and gsh gssg assay kits, by Beyotime (1 mentions)
Cell counting kit 8 assay, by Dojindo Laboratories (1 mentions)
Trizol reagent, by Thermo Fisher Scientific (1 mentions)
Trolox, by Merck Group (1 mentions)
2 2 azobis 2 methylpropionamidine dihydrochloride, by Merck Group (1 mentions)
Ab13533, by Abcam (1 mentions)
Ab218815, by Abcam (1 mentions)
Gsh px, by Nanjing Jiancheng (1 mentions)
α amylase, by Merck Group (1 mentions)
Acarbose, by Merck Group (1 mentions)
Folin ciocalteu reagent, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Catechin hydrate, by Merck Group (1 mentions)
Gallic acid, by Merck Group (1 mentions)
Glycogen, by Nanjing Jiancheng (1 mentions)
Alizarin red s, by Merck Group (1 mentions)
Dulbecco s modified eagle s medium dmem, by Merck Group (1 mentions)
Cck 8 kit, by Dojindo Laboratories (1 mentions)
9 protocols using bicinchoninic acid bca protein assay kit
1
Oxidative Stress Evaluation Protocol
2
Anti-diabetic Potential of Tibetan Barleys
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Highland barley Zangqing 320 (ZQ320), Zangqing 2000 (ZQ2000), Dulihuang (BQ), and Heiqingke (HQK) were donated by Professor Yu Liu, in Tibet Tianyu Agricultural Technology Co., Ltd. (Shigatse, China). Gallic acid, Folin–Ciocalteu reagent, catechin hydrate, α-glucosidase (EC 3.2.1.20) from Saccharomyces cerevisiae, α-amylase (EC 3.2.1.1) from porcine pancreas, acarbose, p-nitrophenyl-α-D-glucopyranoside (pNPG), metformin (Met), and chromatographic grade of standards were purchased from Sigma Chemical Co. (St. Louis, MO, USA). HepG2 human liver cancer cells (ATCC HB-8065) were obtained from ATCC company (Manassas, VA, USA). Fetal bovine serum (FBS) and other cell culture reagents were purchased from Gibco Life Technologies Co. (Grand Island, NY, USA). Glucose, glycogen, and bicinchoninic acid (BCA) protein assay kits were obtained from Nanjing Jiancheng Institute of Bioengineering (Nanjing, Jiangsu, China). Human G6Pase, PEPCK, and GYS2 assay kits were purchased from Shanghai Changjin Biotechnology Co., Ltd. (Shanghai, China).
3
Osteogenic Differentiation Protocol
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ASA VI (purity >99%, National Institute for the Control of Pharmaceuticals and Biological Products, Beijing, China) was dissolved in phosphate-buffered saline (PBS) and stored at −20 °C. Dulbecco’s Modified Eagle’s Medium (DMEM) and fetal bovine serum (FBS) were purchased from Sigma (Sigma-Aldrich Co., USA). A CCK-8 kit was obtained from Dojindo (Kumamoto, Japan). Bicinchoninic acid (BCA) protein assay kit was obtained from Jiancheng (Nanjing, China)and alkaline phosphatase (ALP) activity assay kit was obtained from Wako (Japan). LY294002 was obtained from the Biyuntian Bioengineering Institute (Shanghai, China). Ascorbic acid phosphate, β-glycerophosphate, dexamethasone and alizarin red S were also purchased from Sigma (Sigma-Aldrich Co, USA).
4
Antimicrobial and Biocompatible Biopolymer Composite
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Lysozyme (from eggs, 70,000 U/mg), pancreatin, chitosan (CS, viscosity < 200 mPa.s) and carboxymethyl starch sodium (CMS, MW 50KD) with a substitution degree of 0.1046 were purchased from Aladdin Co., Ltd. (Shanghai, China). Escherichia coli O157:H7 (E. coli, ATCC 25922) and Staphylococcus aureus (S. aureus, ATCC25923) were bought from Sigma–Aldrich Corp., St. Louis, MO, USA. Human umbilical vein endothelial cells (hUVECs) were purchased from the China Center for Type Culture Collection. Lysozyme assay kit and bicinchoninic acid (BCA) protein assay kit were purchased from Jiancheng Bioengineering Institute (Nanjing, China). The Luria–Bertani broth (LB Broth), RPMI1640 culture medium, fetal bovine serum (FBS), Penicillin-Streptomycin solution (10 mg/mL) and Cell Counting Kit-8 (CCK-8) were purchased from Beijing LABLEAD Co., Ltd. (Beijing, China). Ultrapure water from a Milli-Q filtration system (Millipore Corp., Bedford, MA, USA) was used to prepare all solutions.
5
Molecular Mechanisms of Anti-inflammatory Activity
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Pte (≥99%, purity) and 4MR (≥98%, purity) were purchased from Great Forest Biomedical (Hangzhou, China). LPS (Escherichia coli 0111: B4), dimethylsulfoxide (DMSO), and crystal violet were purchased from Sigma-Aldrich (St. Louis, MO, USA). TRIzol reagent was purchased from Takara (Otsu, Shiga, Japan). Nitric oxide (NO) assay kit and bicinchoninic acid (BCA) protein assay kit were obtained from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). Primary antibodies against ERK1/2, phospho-ERK1/2, JNK, phospho-JNK, c-Jun, phospho-c-Jun, p38, phospho-p38, p65, and phospho-p65 were obtained from Cell Signaling Technology (Boston, MA, USA). Anti-β-actin antibody was obtained from AbCam (Cambridge, Britain).
6
Jejunum Oxidative Stress Assessment
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Jejunum samples were homogenized in 0.9% sodium chloride solution on ice and then centrifuged at 3,500 × g for 15 min at a temperature of 4 °C. Both serum and jejunum supernatant solution were used to measure the levels of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), glutathione (GSH), and malondialdehyde (MDA) according to the manufacturer's instructions of corresponding assay kits (Nanjing Jiancheng Bioengineering Institute, Nanjing, China). Protein content was tested with a bicinchoninic acid (BCA) protein assay kit purchased from Nanjing Jiancheng Bioengineering Institute.
7
Detailed Protein Purification Protocol
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The reagents used in this experiment, including sodium chloride, ammonium sulfate, formaldehyde, bromophenol blue, trichloroacetic acid, disodium hydrogen phosphate, sodium dodecylsulfate (SDS), methyl red, sodium acetate, and ammonium persulfate (APS) were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). A bicinchoninic acid (BCA) protein assay kit and a trypsin assay kit were purchased from the Nanjing Jian Cheng Institute of Bioengineering (Nanjing, China). Glucan gel G-50 and Q-agarose gel FF were purchased from Shanghai Cable Bridge Biotechnology Co., Ltd. (Shanghai, China). All chemical reagents were of analytical grade. The RSD of chemical reagents was less than 1 %.
8
Inhibition of α-Glucosidase Activity
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Methanol, ethyl acetate, n−butanol, dichloromethane, petroleum ether, and ethanol were of analytical grade and purchased from Fuyu Fine Chemical Co., Ltd. (Tianjin, China). α−Glucosidase (from yeast), phosphate−buffered saline (PBS), and streptozotocin (STZ) were purchased from Sigma−Aldrich (St Louis, MO, USA). Na2CO3, 4−nitrophenyl α−D−galactopyranoside (PNPG), acarbose, and TBST buffer were obtained from Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). Bicinchoninic acid (BCA) protein assay kit was purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing China). Pre−stained protein marker was obtained from New Cell & Molecular Biotech Co., Ltd. (Suzhou, China). RAC−alpha serine/threonine−protein kinase (AKT1), peroxisome proliferator−activated receptor gamma (PPARG), prostaglandin G/H synthase 2 (PTGS2), β−actin, and HRP−conjugated Affinipure Goat Anti−Mouse IgG (H + L) were purchased from Proteintech Group, Inc. (Rosement, PA, USA).
9
Magnetic Iron Oxide Nanoparticles Synthesis
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Iron (III) chloride hexahydrate (FeCl3·6H2O), sodium oleate, oleic acid, 1-octadecene, poly (allylamine hydrochloride) (PAH; Mw = 15,000 Da), phosphate buffered saline (PBS), tetramethylammonium hydroxide, N-(3-(dimethylamino)propyl-N′-ethylcarbodiimide) hydrochloride (EDC), N-hydroxysulfosuccinimide sodium salt (NHS), 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2 H-tetrazolium bromide (MTT) assay kit and 2ʹ,7ʹ-dichlorohydrofluorescein diacetate (DCFH; ≥ 94%), glucose oxidase (GOx) were purchased from Sigma-Aldrich. 3,3′,5,5′-tetramethylbenzidine (TMB), 2,2′-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), o-phenylenediamine (OPD; 98%), methylene blue (MB), terephthalic acid (TA) and hydrogen peroxide (H2O2) assay kit were obtained from Beyotime Biotechnology. Bicinchoninic acid (BCA) protein assay kit, malondialdehyde (MDA) and protein carbonyl assay kit were obtained from Nanjing Jiancheng Institute of Biological Engineering. Live/dead bacteria viability kit was purchased from Thermo Fisher Scientific. All other chemicals were obtained from Adamas-beta and used without further purification. Deionized (DI) water (Millipore Milli-Q grade, 18.2 MΩ) was used in all the experiments.
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