DMEM and fetal bovine serum (FBS) were obtained from Transgen Biotech (Beijing, China). Exosome-free FBS was from SBI (San Francisco, CA). Ang II, FITC-phalloidin, and FITC-WGA were from Sigma-Aldrich (St. Louis, MO). Antibodies against BNP and ANP were purchased from Abcam (Cambridge, MA), whereas PTEN, p-AKT, total AKT, β-actin, and CD63 were from Proteintech (Chicago, IL). Plasmid purification kits were from TIANGEN (Beijing, China). Chemically synthesized miRNAs, siRNAs, and exosome isolation reagent were obtained from RiboBio (Guangzhou, China).
Dulbecco modified eagle medium (dmem)
Manufactured by Transgene
Sourced in China
DMEM (Dulbecco's Modified Eagle's Medium) is a widely used cell culture medium. It provides essential nutrients and components required for the growth and maintenance of various cell types in vitro.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Transgene (7 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions)
Penicillin streptomycin, by Transgene (3 mentions)
Penicillin streptomycin, by Merck Group (2 mentions)
Rpmi 1640, by Transgene (2 mentions)
Horse serum, by Transgene (2 mentions)
Dulbecco s modi ed eagle s medium dmem, by Thermo Fisher Scientific (2 mentions)
Exosome free fbs, by System Biosciences (2 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions)
Fitc phalloidin, by Merck Group (1 mentions)
Total akt, by Proteintech (1 mentions)
Wga fitc, by Merck Group (1 mentions)
Exosome isolation reagent, by RiboBio (1 mentions)
Plasmid purification kit, by Tiangen Biotech (1 mentions)
Ang 2, by Merck Group (1 mentions)
β actin, by Proteintech (1 mentions)
Sirna, by RiboBio (1 mentions)
P akt, by Proteintech (1 mentions)
Skov3, by Procell (1 mentions)
12 protocols using dulbecco modified eagle medium (dmem)
1
Exosome Isolation and Analysis
2
Ovarian Cancer Cell Culture Protocol
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Four OC cell lines (OVCAR3, SKOV3, A2780, and HO-8910) and human ovarian immortalized nontumorigenic ovarian surface epithelial cells (IOSE) were acquired from Procell Life Science Co., Ltd.,China and cultured in Dulbecco’s modified Eagle medium (DMEM) (TransGen Biotech Co., Ltd., Beijing, China) containing 10% fetal bovine serum (Gibco Company, NY, USA) and 1% penicillin/streptomycin (Sigma-Aldrich Chemical Company., MO, USA). All the cells were incubated in humidified chambers [21 (link)].
3
Goose Embryo Fibroblast Cell Culture Protocol
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Primary goose embryo fibroblasts (GEFs) were derived from ten-day-old goose embryos, and were maintained in Dulbecco’s modified Eagle’s medium (DMEM; TransGen Biotech, Beijing, China) supplemented with 10% newborn calf serum (NCBS; HyClone, Logan, UT, USA) and grown at 37 °C. Baby hamster kidney cells (BHK21) and human fetal kidney cells (HEK 293T) were cultivated in DMEM with 5% fetal bovine serum (FBS; HyClone), at 37 °C with 5% CO2. The TMUV CQ strain (accession: KM233707) was used and its 50% tissue culture infection dose (TCID50) was detected in GEFs as 6.3 × 106 TCID50/0.1 mL [36 (link)]. All one-week-old healthy goslings were purchased from the breeding center of Sichuan Agricultural University, Yaan City, China, and then maintained and observed for three days in laboratory animal rooms prior to experiments. The welfare of the animals was ensured during the sampling process.
4
Culturing Prostate Cell Lines
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The prostate cancer cell lines PC3, DU145, VCaP, 22RV1, C4-2, and LNCaP, and the normal prostate cell line, RWPE, were obtained from the American Type Culture Collection (Manassas, VA, USA). The cells were cultured in RPMI 1640 (TransGen Biotech, Beijing, China) supplemented with 10% fetal bovine serum (TransGen Biotech), in Dulbecco’s modified Eagle’s medium (DMEM, TransGen Biotech), or in keratinocyte serum-free medium containing 5 ng/mL epidermal growth factor and 25 mg/mL bovine pituitary extract (K-SFM, Gibco, Waltham, MA, USA) in an incubator containing 5% CO2 at 37°C.
5
Ovarian Cancer Cell Lines Profiling
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Six ovarian cancer cell lines (OVCAR3, SKOV3, A2780, HO-8910, Caov3, and CP70) and human ovarian immortalized nontumorigenic ovarian surface epithelial cells (IOSE) were obtained from the American type culture collection (ATCC) (Maryland, USA) and cultured in RPMI 1640 (TransGen, Beijing, China) medium or DMEM (TransGen, Beijing, China) supplemented with 10% fetal bovine serum (Gibco, 10%FBS), 1% penicillin/streptomycin (Sigma, USA). All the cells were maintained in humidified chamber at 37°C with 5% CO2.
miR-221 mimics (catalog number: AM16697), miR-221 inhibitor (anti-miR-221, catalog number: AM16812) as well as the negative control were all obtained from Sigma-Aldrich (Sigma, USA). Cell transfection was performed using Lipofectamine 3000 Reagent (Life Technologies, San Diego, CA, USA) based on the manufacturer’s protocol. The CASC15 sequences were ligated into the pEX-2 vector (pEX-CASC15) and pEX-2 vector-control was used as a negative control (pEX) (Catalog number: pEX-2, Genepharma, China).
miR-221 mimics (catalog number: AM16697), miR-221 inhibitor (anti-miR-221, catalog number: AM16812) as well as the negative control were all obtained from Sigma-Aldrich (Sigma, USA). Cell transfection was performed using Lipofectamine 3000 Reagent (Life Technologies, San Diego, CA, USA) based on the manufacturer’s protocol. The CASC15 sequences were ligated into the pEX-2 vector (pEX-CASC15) and pEX-2 vector-control was used as a negative control (pEX) (Catalog number: pEX-2, Genepharma, China).
6
In Vitro Cultivation of Toxoplasma gondii
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The T. gondii RH ΔKu80 strain and derived strains were cultured in vitro by serial passages in human foreskin fibroblasts (HFFs; ATCC, Manassas, VA, USA) or African green monkey kidney cells (Vero cells, a gift from Professor Liu Qun at the Chinese Agricultural University) using Dulbecco’s Modified Eagle’s Medium (DMEM, Macgene, Beijing, China) supplemented with 2% FBS at 37 °C and 5% CO2. Cells were maintained in DMEM supplemented with 10% heat-inactivated fetal bovine serum (TransGen Biotech, Beijing, China) and incubated at 37 °C in a 5% CO2 environment.
7
Maintenance of Mouse Embryonic Stem Cells
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46C mESCs [26 (link)], which were provided by Qi-Long Ying (University of Southern California, USA), were cultured on 0.1% gelatin-coated dishes at 37°C in 5% CO2. The basal media for routine maintenance was Dulbecco's Modified Eagle Medium (DMEM, TransGen Biotech, China) supplemented with 10% Fetal Bovine Serum (FBS, ExCell Bio, Australia), 1× MEM non-essential amino acids (Invitrogen, USA), 2 mM GlutaMax (Invitrogen, USA), 1× sodium pyruvate (Invitrogen, USA), 0.1 mM β-mercaptoethanol (Invitrogen, USA), 1× penicillin/streptomycin (Invitrogen, USA), and 100 units/ml LIF (Millipore, USA). 293T cells were cultured in the same 10% FBS-DMEM except in the absence of LIF.
8
Lumbar Spinal Stenosis Cell Isolation
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LF tissue samples harvested from lumbar spinal stenosis patients were washed in physiological saline, minced and incubated for 1 h at 37 °C in Dulbecco’s Modified Eagle Medium (DMEM; HyClone, USA) containing 0.2% type I collagenase (Sigma, USA). The suspension was filtered using a 70 mm-mesh cell strainer (Falcon, BD, USA) and cells were seeded into the wells of a 60 mm culture dish (NEST, Wuxi, China) containing DMEM supplemented with 10% fetal bovine serum (FBS; Transgen, Beijing, China), 100 U/mL penicillin and 100 pg/mL streptomycin (HyClone). Subsequent experiments were performed using cells that were passaged between 2 and 5 times.
9
Primary Hepatocyte Isolation Protocol
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Liver tissues were digested with collagenase (0.15% in DMEM, Thermo Fisher Scientific, Beijing, China) at room temperature for 15 min. After centrifugation at 3000 rpm for 10 min, cells were collected and cultured in DMEM medium supplemented with 1% penicillin/streptomycin (TransGen Biotech, Beijing, China) and 2% fetal bovine serum (TransGen Biotech, Beijing, China).
10
Cultivation of Ovarian Cell Lines
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Four OC cell lines (OVCAR3, SKOV3, A2780, and HO-8910) and human ovarian immortalized nontumorigenic ovarian surface epithelial cells (IOSE) were acquired from American Type Culture Collection (VA, USA) and cultured in Dulbecco's modi ed Eagle medium (DMEM) (TransGen Biotech Co., Ltd., Beijing, China) containing 10% fetal bovine serum (Gibco Company, NY, USA) and 1% penicillin/streptomycin (Sigma-Aldrich Chemical Company., MO, USA). All the cells were incubated in humidi ed chambers [20] .
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