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Macs enrichment

Manufactured by Miltenyi Biotec
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The MACS enrichment product is a magnetic cell separation system that enables the isolation and purification of specific cell populations from heterogeneous samples. It utilizes magnetic microbeads coated with antibodies or other ligands to target and label the desired cells, which can then be separated using a magnetic field.

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Lab products found in correlation

Facsmelody, by BD (1 mentions) Anti cd11b macs beads, by Miltenyi Biotec (1 mentions) Nextera xt dna library preparation kit, by Illumina (1 mentions) Qiashredder column, by Qiagen (1 mentions) Novaseq 6000, by Illumina (1 mentions) Rneasy micro extraction kit, by Qiagen (1 mentions) Smart seq v4 ultra low input rna kit, by Illumina (1 mentions)

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MACS magnetic enrichment column (2 citations)

4 protocols using macs enrichment

1

Allogeneic Bone Marrow Transplantation

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Allogeneic BM transplantation was performed as previously described (42 (link)). Recipients were given 5 × 106 T cell–depleted BM cells directly after lethal TBI. T cell depletion of BM cells was performed as previously described (43 (link)). T cell doses (MACS enrichment, Miltenyi) varied depending on the transplant model.
Fischer J.C., Bscheider M., Eisenkolb G., Lin C.C., Wintges A., Otten V., Lindemans C.A., Heidegger S., Rudelius M., Monette S., Porosnicu Rodriguez K.A., Calafiore M., Liebermann S., Liu C., Lienenklaus S., Weiss S., Kalinke U., Ruland J., Peschel C., Shono Y., Docampo M., Velardi E., Jenq R.R., Hanash A.M., Dudakov J.A., Haas T., van den Brink M.R, & Poeck H. (2017). RIG-I/MAVS and STING signaling promote gut integrity during irradiation- and immune-mediated tissue injury. Science translational medicine, 9(386), eaag2513.
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2

Allogeneic Bone Marrow Transplantation

Cited 1 time
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Allogeneic BM transplantation was performed as previously described (42 (link)). Recipients were given 5 × 106 T cell–depleted BM cells directly after lethal TBI. T cell depletion of BM cells was performed as previously described (43 (link)). T cell doses (MACS enrichment, Miltenyi) varied depending on the transplant model.
Fischer J.C., Bscheider M., Eisenkolb G., Lin C.C., Wintges A., Otten V., Lindemans C.A., Heidegger S., Rudelius M., Monette S., Porosnicu Rodriguez K.A., Calafiore M., Liebermann S., Liu C., Lienenklaus S., Weiss S., Kalinke U., Ruland J., Peschel C., Shono Y., Docampo M., Velardi E., Jenq R.R., Hanash A.M., Dudakov J.A., Haas T., van den Brink M.R, & Poeck H. (2017). RIG-I/MAVS and STING signaling promote gut integrity during irradiation- and immune-mediated tissue injury. Science translational medicine, 9(386), eaag2513.
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3

CD8+ T cell Apoptosis Assay in Tumor-infiltrating G-MDSCs

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CD8+ T cells were positively selected from spleens of naive 8- to 12-week-old C57BL/6 female mice using Miltenyi MACS Enrichment according to the manufacturer’s protocol. Enriched CD8+ T cells were activated in vitro for 72 h using 1 µg/ml anti-CD3 and 1 µg/ml anti-CD28 to induce Fas expression. Tumor-infiltrating G-MDSCs from DIO mice were sort-purified on a BD FACSMelody after being pre-enriched using anti-CD11b MACS beads from Miltenyi, as per the manufacturer’s protocol. Prior to co-culture, purified G-MDSCs were incubated in the presence or absence of 50 µg/ml anti-FasL neutralizing antibody for 1 h. In vitro activated CD8+ T cells were then co-cultured with G-MDSCs in the continued presence or absence of anti-FasL for 24 h. Cells were harvested and stained for flow cytometry to evaluate CD8 T cell apoptosis as indicated above.
Gibson J.T., Orlandella R.M., Turbitt W.J., Behring M., Manne U., Sorge R.E, & Norian L.A. (2020). Obesity-Associated Myeloid-Derived Suppressor Cells Promote Apoptosis of Tumor-Infiltrating CD8 T Cells and Immunotherapy Resistance in Breast Cancer. Frontiers in Immunology, 11, 590794.
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4

Investigating Treg Transcriptomic Changes in Allograft Rejection

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Fully MHC-mismatched (Balb/c, H-2d) skin was grafted onto H-2b B6.129(Cg)-Foxp3tm4(YFP/icre)Ayr xTIGITfl/fl recipients or wild type littermate controls. Animals were treated on days 0, 2, 4, and 6 with CTLA-4Ig + TIGIT agonist and sacrificed on day 10. DLN were recovered, homogenized and CD4+ T cells were negatively selected for using MACS enrichment (Miltenyi) prior to sorting on CD25+YFP+GITR+ (conditional knock-out) or CD25+YFP−GITR+ (WT) Treg. 5,000 purified Treg from 7 cKO and 7 WT animals were lysed using QIAshredder columns (Qiagen) and mRNA was extracted (RNeasy Micro extraction kit, Qiagen). Library preparation (TakaraBio’s SMART-Seq v4 Ultra Low Input RNA Kit with Illumina’s Nextera XT DNA Library Preparation Kit) and sequencing (Illumina NovaSeq 6000 and Illumina MiSeq) were performed by the Genomics Core at Emory University. Data was analyzed in R using the DESeq2 package from Bioconductor27 (link), Gene Set Enrichment Analysis (v4.2.3 for Mac GSEA) and Morpheus (both publicly available from the Broad Institute). Gene ontology analysis of differentially expressed genes was performed using PANTHER28 (link),29 (link) and GORILLA30 (link) software.
Hartigan C.R., Tong K.P., Liu D., Laurie S.J, & Ford M.L. (2023). TIGIT agonism alleviates costimulation blockade resistant rejection in a Treg-dependent manner. American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons, 23(2), 180-189.
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