Luminex analyzer

Manufactured by Merck Group

The Luminex analyzer is a multiplex detection system that utilizes the principles of flow cytometry to perform simultaneous quantitative analysis of multiple analytes in a single sample. It employs color-coded magnetic microspheres coated with specific capture reagents to enable the detection and measurement of various biomolecules, such as proteins, nucleic acids, and other analytes. The core function of the Luminex analyzer is to provide a robust and efficient platform for high-throughput, multiplexed assays in a wide range of research and diagnostic applications.

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Lab products found in correlation

Human angiogenesis growth factor magnetic bead panel kit, by Merck Group (2 mentions)

Close spelling variants of this product

Luminex® 200 analyzer system Luminex 200 microbead analyzer Analyzer 3.1 Luminex 200 machine Luminex MAGPIX analyzer Luminex 200 analyzer

4 protocols using luminex analyzer

Cytokine Secretion in HAEC with VEGF and Aspalatone

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Analysis of various cytokines secreted by HAECs treated with VEGF (10 ng/mL) in absence or presence of aspalatone (50 μM and 100 μM) was analyzed by using a Human Angiogenesis/Growth Factor Magnetic bead panel kit from Millipore following manufacturer's instructions. Briefly, the culture media was collected, concentrated by lyophilization, and incubated with the labelled magnetic beads. After overnight incubation, the beads were counterstained with Streptavidin-Phycoerythrin and analyzed with a Luminex™ analyzer from Millipore. The results are expressed as pg/mL based on the standard curve generated with the standards.

Sonowal H., Pal P.B., Shukla K, & Ramana K.V. (2017). Aspalatone Prevents VEGF-Induced Lipid Peroxidation, Migration, Tube Formation, and Dysfunction of Human Aortic Endothelial Cells. Oxidative Medicine and Cellular Longevity, 2017, 2769347.

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Quantifying Angiogenic Cytokines in HUVEC

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Analysis of angiogenic cytokines secreted by HUVECs treated without or with VEGF (10 ng/mL) ± vialinin (5 μM) was performed by using a human angiogenesis/growth factor magnetic bead panel kit from Millipore (number HAGP1MAG-12 K) following the manufacturer's protocol. Briefly, after 24 h of treatment of HUVECS with VEGF ± vialinin A, the media were collected by centrifugation and filtered using a 0.2 μM syringe filter. The media were then frozen in −80°C and concentrated by a using a vacuum evaporator. Equal amounts of resuspended lyophilized media were then incubated with the labeled magnetic beads. After incubating overnight, the beads were counterstained with streptavidin-phycoerythrin and analyzed using a Luminex analyzer from Millipore. Data were analyzed using the xPONENT software, and the results are expressed as pg/mL.

Sonowal H., Shukla K., Kota S., Saxena A, & Ramana K.V. (2018). Vialinin A, an Edible Mushroom-Derived p-Terphenyl Antioxidant, Prevents VEGF-Induced Neovascularization In Vitro and In Vivo. Oxidative Medicine and Cellular Longevity, 2018, 1052102.

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Cytokine Profiling of Macrophage Responses

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RAW 264.7 murine macrophages or human THP-1 monocyteswere pre-treated with 2-HF overnight in 0.1% serum-containing media. The next day, the cells were stimulated with 1μg/mL LPS in 0.1% serum-containing media for 24 h. After 24 h treatment, an equal amount of media was collected, cleared by centrifugation to remove debris and stored in −80°C. The media was then concentrated 10x using a vacuum evaporator (Savant SC210A, Thermo scientific) and 25 μL media from each condition was used in the assay. The media was incubated with the labeled magnetic beads provided with the mouse multiplex (#MCYTOMAG-70K-PMX) or human multiplex (#HCYTOMAG-60K) cytokine chemokine kit from Millipore Sigma. After incubating overnight at 4°C, the beads were washed twice with wash buffer, incubated with detection antibodies, and counterstained with Streptavidin PE. Acquisition and analysis were carried out using a Luminex analyzer from Millipore and data are presented as pg/mL based on the standard curve generated using the standards provided with the kit.

Sonowal H, & Ramana K.V. (2020). 2’-Hydroxyflavanone prevents LPS-induced inflammatory response and cytotoxicity in murine macrophages. Toxicology in vitro : an international journal published in association with BIBRA, 69, 104966.

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Inflammation Cytokine Profiling of HUVECs

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Human Angiogenesis/Growth Factor Magnetic bead panel kit from Millipore was used to determine various inflammatory cytokines and chemokines. HUVECs were stimulated with HG (25mM) in the absence or presence of didymin (20μM) for 24 h. After completion of incubation period, equal amounts of cell culture media were collected and concentrated from each group. The concentrated media was incubated with the labeled magnetic beads overnight. Next day, the magnetic beads were counterstained with Streptavidin-phycoerythrin and cytokine levels were analyzed with a Luminex analyzer from Millipore. The results are expressed as pg/mL based on the standard curve generated with the standards.

Shukla K., Sonowal H., Saxena A, & Ramana K.V. (2018). Didymin prevents hyperglycemia-induced human umbilical endothelial cells dysfunction and death. Biochemical pharmacology, 152, 1-10.

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