MCF-7PR cells were transfected with HER2 siRNA (Cell Signaling Technologies, Danvers, MA) using siPORT (Ambion Inc, Austin, TX) transfection reagent as per manufacturer’s protocol. Briefly, approximately 0.2 × 106 cells/well were plated in 6 well plate and left overnight for attachment. Next day, cells were transfected with 100 nM HER2 siRNA or scrambled siRNA using siPORT reagent and 8 h post transfection, cells were treated with 20 nM of paclitaxel for additional 72 h. The cells were collected after treatment and processed for western blot analysis.
Her2 sirna
Manufactured by Cell Signaling Technology
HER2 siRNA is a small interfering RNA (siRNA) designed to target the HER2 (human epidermal growth factor receptor 2) gene. The core function of this product is to enable the specific knockdown of HER2 expression in cell-based experiments.
Automatically generated - may contain errors
2 protocols using her2 sirna
1
HER2 Silencing and Paclitaxel Treatment in MCF-7PR Cells
2
Atovaquone Reduces HER2 Expression
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