Flash 2000 instrument

Electrospray Ionisation Mass Spectrometry (ESI-MS) analysis was done using a Perkin Elmer Sciex API3000 Instrument (Applied Biosystem/MDS Sciex, Toronto, ON, Canada) with Analytes 1.4 software. Analytical RP-HPLC analysis was done on a Shimadzu (Kyoto, Japan) instrument (DGU-20A5, LC-20AB, SIL-20ACHT, SPD-M10AVP) with a flow rate of 1 mL/min and detection at 214 nm. Analytical HPLC analysis was done with a 0%–100% gradient of analytical grade solvent A (0.1% TFA in water) to solvent B (90% MeCN, 10% water, 0.1% TFA) over 50 min and a Vydac analytical C18 column (218TP54; 5 µm, 4.6 mm × 250 mm). Preparative RP-HPLC analysis was done with a Shimadzu Instrument (Tokyo, Japan) at a flow rate of 20 mL/min and a Vydac C18 column, with detection at 230 nm. Particle size was measured by dynamic light scattering (DLS; Malvern Zetasizer Nano Series with DTS software). Transmission electron microscopy (TEM; HT7700 Exalens, HITACHI Ltd., Tokyo, Japan) was performed at the Australian Microscopy & Microanalysis Research Facility, Centre for Microscopy and Microanalysis, The University of Queensland (UQ). Element microanalysis (EA) was performed in the School of Chemistry and Molecular Biosciences, UQ, using a FLASH 2000 instrument (Thermo Fisher Scientific, Waltham, MA, USA).

All chemicals purchased from commercial sources were of analytical grade and were used without further purification. The chemicals 3-fluoro-2-hydroxybenzaldehyde and 5-fluoro-2-hydroxybenzaldehyde were prepared by a modified procedure according to Ferguson et al. [43 (link)]. Cell cultures were purchased from the Czech National Collection of Type Cultures. Infrared spectra were recorded using the ATR (attenuated total reflectance) technique on a Nicolet 6700 FT-IR spectrometer from Thermo Scientific (Waltham, MA, USA) in the 600–4000 cm⁻¹ range. Elemental analysis was performed with the help of a Flash2000 instrument from Thermo Scientific. ¹H-, ¹³C- and ¹⁹F-NMR spectra were measured on a Varian Mercury 300 MHz instrument (Palo Alto, CA, USA) using DMSO-d₆ as solvent and TMS (tetramethylsilane) as internal standard. Melting points were determined on a Büchi B-540 apparatus (Büchi Labortechnik AG, Flawil, Switzerland). The conductivities of the copper(II) complexes were evaluated in DMSO solutions (10⁻³ mol/L, 25 °C) on a WTW LF 530 conductometer (Weilheim, Germany).