Htb 132tm

MDA-MB-231 (ATCC^® HTB-26TM) and MDA-MB-468 (ATCC^® HTB-132^TM), two TNBC cell types, were acquired from ATCC and kept per ATCC’s maintenance instructions. Both cell lines were grown in 75-mL tissue culture (TC) flasks as monolayers at 37 °C in a humidified 5% CO₂ incubator, occasionally subculturing with trypsin/EDTA. 4 mM L-glutamine, 10% heat-inactivated FBS (v/v), and 1% penicillin/streptomycin salt solution (100 U/mL and 0.1 mg/mL, respectively) were added to the complete growth DMEM. The experimental media was DMEM supplemented with 2.5% heat-inactivated FBS [35 (link)].

Four HER2 overexpressing cell lines were used in this study. The ovarian adenocarcinoma cell line SKOV-3 (ATCC, HTB-77™), purchased from American Type Culture Collection (ATCC, Manassas, VA, USA), the human breast adenocarcinoma cell line SK-BR-3, kindly provided by the Department of Biochemistry, Institute for Cancer Research, Norwegian Radium Hospital, Oslo University Hospital, Oslo, Norway, the human ovarian carcinoma cell line HOC-7, kindly provided by Dr. Yvonne Anderson, Department of Tumor Biology, Institute for Cancer Research, Norwegian Radium Hospital, Oslo University Hospital, Oslo, Norway, and the rat ovarian cancer cell line NuTu-19, originally a gift from Dr. A.L Major, University of Geneva, Switzerland [22 ]. The HER2 low expressing human breast adenocarcinoma cell line MDA-MB-468 (ATCC, HTB-132^TM) was obtained from ATCC. SKOV-3 and SK-BR-3 cells were cultured in McCoy's 5A medium while HOC-7 and NuTu-19 cells were cultured in RPMI 1640 medium. Both media were obtained from Sigma-Aldrich (St. Louis MO) and modified as previously described [23 ]. The MDA-MB-468 cells were cultured in Leibovitz`s L-15 medium (Lonza, Basel, Switzerland) modified as previously described [23 ] with free gas exchange with atmospheric air.

MDA-MB-231 cells were a gift from Dr. Robert S. Kerbel (Sunnybrook Health Sciences Centre, Toronto, Canada^{9 (link)} and cultured in RPMI (GIBCO) supplied with 5% foetal bovine serum (FBS) (Gibco). L cells were purchased from ATCC (CRL-2648^TM) and cultured in Dulbecco’s modified Eagle’s medium (DMEM) (HyClone) with 10% FBS. HEK293T cells were purchased from ATCC (CRL-3216™) were cultured in DMEM with 10% FBS supplemented with GlutaMAX. MDA-MB-468 cells were purchased from ATCC (HTB-132^TM) and were cultured in the DMEM/F-12 (1:1) medium (Hyclone) with 10% FBS. Mouse breast cancer cell lines EpRas were a gift from Dr. Martin Oft and were cultured in the DMEM (HyClone) with 10% FBS. Human bladder cancer cell lines T24 (HTB-4^TM) and TCCSUP (HTB-5^TM) were purchased from ATCC and cultured in McCoy 5 A (Thermo Fisher) and RMPI (GIBCO) with 5% FBS, respectively. All cells were maintained at 37 ^oC in a humidified atmosphere containing 5% CO₂. During the course of this study, all cell lines were routinely tested for mycoplasma contamination. All cell lines were authenticated by STR profiling.